加州大学医学院 Teh Ping Lin 博士早在1966年即利用小鼠受精卵原核显微注射技术,系统地研究了微注射不同量外源物质(石蜡油、牛γ-球蛋白溶液)对小鼠胚胎受体移植后的分裂、发育的影响程度,并预见该技术用于研究小鼠或其它种类哺乳动...加州大学医学院 Teh Ping Lin 博士早在1966年即利用小鼠受精卵原核显微注射技术,系统地研究了微注射不同量外源物质(石蜡油、牛γ-球蛋白溶液)对小鼠胚胎受体移植后的分裂、发育的影响程度,并预见该技术用于研究小鼠或其它种类哺乳动物胚胎分化及遗传效应具有潜在价值。直到1980年,随着分子生物学技术的迅速发展,Gordon 等人首先采用显微注射法育成带有人胸苷激酶基因这一外源 DNA 片断的'新型'转基因小鼠后。展开更多
Objectives: To evaluate the hospital’s cryopreservation protocol. Study design: A retrospective cohort analysis of 30 conventional IVF and 44 ICSI cycles in an assisted conception unit at a tertiary referral hospital...Objectives: To evaluate the hospital’s cryopreservation protocol. Study design: A retrospective cohort analysis of 30 conventional IVF and 44 ICSI cycles in an assisted conception unit at a tertiary referral hospital. All supernumerary embryos were cryopreserved at the pronuclear or blastomere stage. The survival, morphology, implantation and pregnancy rates were evaluated. The χ2 test and Fishers exact test were used to determine the statistical significance. Results: A total of 327 pronuclear and cleavage stage embryos were cryopreserved. The post thaw survival rates of 107 conventional IVF and 220 ICSI embryos were 90.6%and 69.0%, respectively. Of the thawed cleavage stage embryos from 43 IVF and 88 ICSI cycles, 90.6%and 69.3%were intact, respectively. Of the thawed pronuclear stage embryos from 64 IVF and 132 ICSI cycles, 90.6%and 68.9%were intact with cleavage rates of 57.8%and 56%, respectively. In the final 27 conventional IVF and 41 ICSI frozenthawed embryo transfer cycles, the pregnancy rates were 18.5%and 19.5%, respectively. Conclusions: The adopted cryopreservation protocol flexibly allows for the selection of cleavagestage embryos for fresh embryo transfer and the cryopreservation of all supernumerary embryos at the pronuclear or the cleavage stage in a single cycle with satisfactory pregnancy rates. Further validation of this protocol is required.展开更多
文摘加州大学医学院 Teh Ping Lin 博士早在1966年即利用小鼠受精卵原核显微注射技术,系统地研究了微注射不同量外源物质(石蜡油、牛γ-球蛋白溶液)对小鼠胚胎受体移植后的分裂、发育的影响程度,并预见该技术用于研究小鼠或其它种类哺乳动物胚胎分化及遗传效应具有潜在价值。直到1980年,随着分子生物学技术的迅速发展,Gordon 等人首先采用显微注射法育成带有人胸苷激酶基因这一外源 DNA 片断的'新型'转基因小鼠后。
文摘Objectives: To evaluate the hospital’s cryopreservation protocol. Study design: A retrospective cohort analysis of 30 conventional IVF and 44 ICSI cycles in an assisted conception unit at a tertiary referral hospital. All supernumerary embryos were cryopreserved at the pronuclear or blastomere stage. The survival, morphology, implantation and pregnancy rates were evaluated. The χ2 test and Fishers exact test were used to determine the statistical significance. Results: A total of 327 pronuclear and cleavage stage embryos were cryopreserved. The post thaw survival rates of 107 conventional IVF and 220 ICSI embryos were 90.6%and 69.0%, respectively. Of the thawed cleavage stage embryos from 43 IVF and 88 ICSI cycles, 90.6%and 69.3%were intact, respectively. Of the thawed pronuclear stage embryos from 64 IVF and 132 ICSI cycles, 90.6%and 68.9%were intact with cleavage rates of 57.8%and 56%, respectively. In the final 27 conventional IVF and 41 ICSI frozenthawed embryo transfer cycles, the pregnancy rates were 18.5%and 19.5%, respectively. Conclusions: The adopted cryopreservation protocol flexibly allows for the selection of cleavagestage embryos for fresh embryo transfer and the cryopreservation of all supernumerary embryos at the pronuclear or the cleavage stage in a single cycle with satisfactory pregnancy rates. Further validation of this protocol is required.
