The developmental process of Myeloma cells under a digital optical microscope has been inspected and monitored by using time-lapsed recording technique. Myeloma cells were cultured in medium contained 20% and 50% of F...The developmental process of Myeloma cells under a digital optical microscope has been inspected and monitored by using time-lapsed recording technique. Myeloma cells were cultured in medium contained 20% and 50% of Fetal Bovine Serum （FBS）, respectively. Inspection and monitoring for 6 hours showed the effect of the FBS to mobility, proliferation rate, and development of cell cycle phases of Myeloma cells. Using time-lapsed data, the speed of cells was 3.5-6.0 pm/s when using FBS 20% and increased to 5.0-8.0 p.m/s when using FBS 50%. The rate of cells decreases from 2 cells/hr when using FBS 20% to 1/6 cells/br when using FBS 50%. The cells division process is signified by the change in gray level and it took every 50-70 minutes.展开更多
Suspended particulate matter （SPM） levels in ambient air were monitored at Mandi Gobindgarh, an industrial town of Punjab, India located on the National Highway-1 during November 2001 to March 2002 covering spring a...Suspended particulate matter （SPM） levels in ambient air were monitored at Mandi Gobindgarh, an industrial town of Punjab, India located on the National Highway-1 during November 2001 to March 2002 covering spring and winter seasons to check the variation of SPM and its constituents in the town. The maximum levels of SPM varied between 594 μg/m^3 to 620 μg/m^3 at selected monitoring sites while the minimum levels varied between 209μg/m^3 to 220 μg/m^3. These values were observed always above the National Ambient Air Quality Standards （NAAQS） set by the State regulatory body. Major sources of SPM were identified as the industrial activity and traffic plying on the national highway. Collected SPM samples were further analyzed for the ignitable matter as loss on ignition （LOI） and organic tarry matter （OTM） content. Ignitable component of the SPM constituted about 45% and tarry matter in the ambient air was about 12%. Effects of meteorological parameters like temperature, wind direction and wind speed on SPM levels are discussed.展开更多
文摘The developmental process of Myeloma cells under a digital optical microscope has been inspected and monitored by using time-lapsed recording technique. Myeloma cells were cultured in medium contained 20% and 50% of Fetal Bovine Serum （FBS）, respectively. Inspection and monitoring for 6 hours showed the effect of the FBS to mobility, proliferation rate, and development of cell cycle phases of Myeloma cells. Using time-lapsed data, the speed of cells was 3.5-6.0 pm/s when using FBS 20% and increased to 5.0-8.0 p.m/s when using FBS 50%. The rate of cells decreases from 2 cells/hr when using FBS 20% to 1/6 cells/br when using FBS 50%. The cells division process is signified by the change in gray level and it took every 50-70 minutes.
文摘Suspended particulate matter （SPM） levels in ambient air were monitored at Mandi Gobindgarh, an industrial town of Punjab, India located on the National Highway-1 during November 2001 to March 2002 covering spring and winter seasons to check the variation of SPM and its constituents in the town. The maximum levels of SPM varied between 594 μg/m^3 to 620 μg/m^3 at selected monitoring sites while the minimum levels varied between 209μg/m^3 to 220 μg/m^3. These values were observed always above the National Ambient Air Quality Standards （NAAQS） set by the State regulatory body. Major sources of SPM were identified as the industrial activity and traffic plying on the national highway. Collected SPM samples were further analyzed for the ignitable matter as loss on ignition （LOI） and organic tarry matter （OTM） content. Ignitable component of the SPM constituted about 45% and tarry matter in the ambient air was about 12%. Effects of meteorological parameters like temperature, wind direction and wind speed on SPM levels are discussed.
