[Objective] The study aimed to establish a fast and accurate method to detect the polymorphism of the 12^th exon of equine MxA gene. [Method] The 12^th exon of MxA gene was amplified by mismatch PCR and the products w...[Objective] The study aimed to establish a fast and accurate method to detect the polymorphism of the 12^th exon of equine MxA gene. [Method] The 12^th exon of MxA gene was amplified by mismatch PCR and the products were analyzed by restriction fragment length polymorphism （RFLP） to determine the point mutation at the 1 790 nt of MxA cDNA. The sequence of the PCR products was also analyzed. [Result] There were three genotypes （AA, AB and BB） in the 12^th exon of equine MxA gene; the 2 081 nt of MxA cDNA mutated from G to C, correspondingly changing the 562^th amino acid of the coding region of MxA protein from tryptophan to cysteine; the specific sequence of the PCR products amplified by mismatch PCR-RFLP was consistent with the analysis results of RFLP. [ Conclusion] The mismatch PCR-RFLP was an easy method with accurate results to detect the polymorphism of the 12^th exon of equine MxA gene.展开更多
The purpose of the study was to explore the factors mediating college students＇ radicalization. A sample of 1,116 students was drawn from four different Jordanian universities. The construct validity of the scale was...The purpose of the study was to explore the factors mediating college students＇ radicalization. A sample of 1,116 students was drawn from four different Jordanian universities. The construct validity of the scale was estimated by calculating the correlation between the radicalization items and the negative emotion items. A positive significant relationship was found （0.12, a = 0.000）, a sign of validity of the scale. The scale reliability was also strong and was estimated at 0.90 using Cronbach Alpha. Factor analysis produced five factors explaining 45% of the total variance of radicalization. The first factor labeled ＂political radicalization＂ explained 18.5% of the variance, the second factor ＂religious radicalization＂ explained 12.7%, the third factor ＂violent radicalization＂ explained 6.4% of the variance, the fourth ＂group radicalization＂ accounted for 4%, and the fifth factor ＂social radicalization＂ only accounted for 3% of the total variance. Significant differences in student radicalization were found according to the geographical region of the university （North, Center and South）, F = 14, a = 0.000. However, no significant differences were found in radicalization as it relates to gender, and type of college （i.e., Humanities vs. Pure Sciences）.展开更多
A linear mixed model is used to determine the explaining infant mortality rate data of United Nations countries. The HDI （human development index） has a significant negative linear relationship with infant mortality...A linear mixed model is used to determine the explaining infant mortality rate data of United Nations countries. The HDI （human development index） has a significant negative linear relationship with infant mortality rate. United Nations data shows that the infant mortality rate has a descending trend over the period 1990-2010. This study aims to assess the value of the HDI as a predictor of infant mortality rate. Findings in the paper suggest that significant percentage reductions in infant mortality might be possible for countries for controlling the HDI.展开更多
As a specific gene of fish,cytochrome P450c17-Ⅱ(CYP17-Ⅱ) gene plays a key role in the growth,development and reproduction level of fish.In this study,the single-stranded conformational polymorphism(SSCP) technique w...As a specific gene of fish,cytochrome P450c17-Ⅱ(CYP17-Ⅱ) gene plays a key role in the growth,development and reproduction level of fish.In this study,the single-stranded conformational polymorphism(SSCP) technique was used to characterize polymorphisms within the coding region of CYP17-Ⅱ gene in a population of 75 male Japanese flounder(Paralichthys olivaceus).Three single nucleotide polymorphisms(SNPs) were identified in CYP17-Ⅱ gene of Japanese flounder.They were c.G594A(p.G188R),c.G939A and c.G1502A(p.G490D).SNP1(c.G594A),located in exon 4 of CYP17-Ⅱ gene,was significantly associated with gonadosomatic index(GSI).Individuals with genotype GG of SNP1 had significantly lower GSI(P < 0.05) than those with geno-type AA or AG.SNP2(c.G939A) located at the CpG island of CYP17-Ⅱ gene.The mutation changed the methylation of exon 6.Indi-viduals with genotype AA of SNP2 had significantly lower serum testosterone(T) level and hepatosomatic index(HSI) compared to those with genotype GG.The results suggested that SNP2 could influence the reproductive endocrine of male Japanese flounder.How-ever,the SNP3(c.G1502A) located in exon 9 did not affect the four measured reproductive traits.This study showed that CYP17-Ⅱ gene could be a potentially useful candidate gene for the research of genetic breeding and physiological aspects of Japanese flounder.展开更多
基金Supported by the Natural Science Foundation of Inner Mongolia Au-tonomous Region (200508010413)~~
文摘[Objective] The study aimed to establish a fast and accurate method to detect the polymorphism of the 12^th exon of equine MxA gene. [Method] The 12^th exon of MxA gene was amplified by mismatch PCR and the products were analyzed by restriction fragment length polymorphism （RFLP） to determine the point mutation at the 1 790 nt of MxA cDNA. The sequence of the PCR products was also analyzed. [Result] There were three genotypes （AA, AB and BB） in the 12^th exon of equine MxA gene; the 2 081 nt of MxA cDNA mutated from G to C, correspondingly changing the 562^th amino acid of the coding region of MxA protein from tryptophan to cysteine; the specific sequence of the PCR products amplified by mismatch PCR-RFLP was consistent with the analysis results of RFLP. [ Conclusion] The mismatch PCR-RFLP was an easy method with accurate results to detect the polymorphism of the 12^th exon of equine MxA gene.
文摘The purpose of the study was to explore the factors mediating college students＇ radicalization. A sample of 1,116 students was drawn from four different Jordanian universities. The construct validity of the scale was estimated by calculating the correlation between the radicalization items and the negative emotion items. A positive significant relationship was found （0.12, a = 0.000）, a sign of validity of the scale. The scale reliability was also strong and was estimated at 0.90 using Cronbach Alpha. Factor analysis produced five factors explaining 45% of the total variance of radicalization. The first factor labeled ＂political radicalization＂ explained 18.5% of the variance, the second factor ＂religious radicalization＂ explained 12.7%, the third factor ＂violent radicalization＂ explained 6.4% of the variance, the fourth ＂group radicalization＂ accounted for 4%, and the fifth factor ＂social radicalization＂ only accounted for 3% of the total variance. Significant differences in student radicalization were found according to the geographical region of the university （North, Center and South）, F = 14, a = 0.000. However, no significant differences were found in radicalization as it relates to gender, and type of college （i.e., Humanities vs. Pure Sciences）.
文摘A linear mixed model is used to determine the explaining infant mortality rate data of United Nations countries. The HDI （human development index） has a significant negative linear relationship with infant mortality rate. United Nations data shows that the infant mortality rate has a descending trend over the period 1990-2010. This study aims to assess the value of the HDI as a predictor of infant mortality rate. Findings in the paper suggest that significant percentage reductions in infant mortality might be possible for countries for controlling the HDI.
基金supported by the Natural Science Foun-dation of Shandong Province,China(No.ZR2009DQ011)the New Teacher Special Fund of Doctor of the Ministry of Education of China(No.20090132120006)the Postdoctoral Innovative Projects of Shandong Province,China(No.200702039)
文摘As a specific gene of fish,cytochrome P450c17-Ⅱ(CYP17-Ⅱ) gene plays a key role in the growth,development and reproduction level of fish.In this study,the single-stranded conformational polymorphism(SSCP) technique was used to characterize polymorphisms within the coding region of CYP17-Ⅱ gene in a population of 75 male Japanese flounder(Paralichthys olivaceus).Three single nucleotide polymorphisms(SNPs) were identified in CYP17-Ⅱ gene of Japanese flounder.They were c.G594A(p.G188R),c.G939A and c.G1502A(p.G490D).SNP1(c.G594A),located in exon 4 of CYP17-Ⅱ gene,was significantly associated with gonadosomatic index(GSI).Individuals with genotype GG of SNP1 had significantly lower GSI(P < 0.05) than those with geno-type AA or AG.SNP2(c.G939A) located at the CpG island of CYP17-Ⅱ gene.The mutation changed the methylation of exon 6.Indi-viduals with genotype AA of SNP2 had significantly lower serum testosterone(T) level and hepatosomatic index(HSI) compared to those with genotype GG.The results suggested that SNP2 could influence the reproductive endocrine of male Japanese flounder.How-ever,the SNP3(c.G1502A) located in exon 9 did not affect the four measured reproductive traits.This study showed that CYP17-Ⅱ gene could be a potentially useful candidate gene for the research of genetic breeding and physiological aspects of Japanese flounder.
