[Objective] This study aimed to establish a pre-column derivatization HPLC method for the identification and analysis of monosaccharide composition of Pu-erh tea polysaccharide. [Method] Pu-erh tea polysaccharide was ...[Objective] This study aimed to establish a pre-column derivatization HPLC method for the identification and analysis of monosaccharide composition of Pu-erh tea polysaccharide. [Method] Pu-erh tea polysaccharide was extracted using the wa- ter extraction method, further isolated and purified by DEAE cellulose-52 columns. The obtained tea polysaccharide and four components TPS1, TPS2, TPS3 and TPS, were first derived by 1-phenyl-3-methyl-5-pyrazolone （PMP）, and then the PMP derivatives of monosaccharide were analyzed by high performance liquid chromatog- raphy （HPLC）. [Result] Pu-erh tea polysaccharide contained eight kinds of monosac- chaddes （mannose, rhamnose, glucuronic acid, galacturonic acid, grucose, galactose, arabinose, fucose）, without xylose; so it was the same with TPS1; each of TPS2, TPS3 and TPS4 contained seven monosaccharides, while no fucose. [Conclusion] This method is simplified and rapid, which can be used to determine the monosac- charide composition of Pu-erh tea polysaccharide and monosaccharide content.展开更多
Several Doppler shift estimators, including mean logarithm envelope difference (MLED) method, auto-correlation function (ACF) method, zero crossing rate (ZCR) method and mean square phase difference (MSPD) method were...Several Doppler shift estimators, including mean logarithm envelope difference (MLED) method, auto-correlation function (ACF) method, zero crossing rate (ZCR) method and mean square phase difference (MSPD) method were discussed and compared. The estimation principle and theoretical estimation bias of these estimators under Rayleigh fading channels were analyzed; furthermore, the Cramer Rao bound (CRB) of Doppler shift estimation was deduced, and a novel modification method based on two-dimensional polynomial fitting was proposed to reduce the Doppler shift estimation bias. We verified our algorithms with the Monte Carlo computer simulation; simulation results showed better variance performance of modified methods than those of the original methods. In addition, the applicable situations of these estimators were discussed.展开更多
This paper analyzed the existing methods of wave measurement, and described the advantages of GPS applied in measuring the wave. The equations of absolute velocity estimation were discussed, focusing on two methods wi...This paper analyzed the existing methods of wave measurement, and described the advantages of GPS applied in measuring the wave. The equations of absolute velocity estimation were discussed, focusing on two methods with Doppler shill. The error sources and their effect on velocity estimation were analyzed. Then, some tests were carried on to simulate dynamic velocity determination using static data Based on the high-frequency carrier-phase derived Doppler observations, the velocity has been estimated to the precision of 1 cm/s or so, even to the mm/s level. And with the receiver generated Doppler measurements, the precision can reach 3 - 15 cm/s.展开更多
[Objective] This study aimed to optimize the extraction process parameters of Pu-erh tea polysaccharide. [Method] Single-factor experiment was carried out to analyze the influences of three main factors, including ext...[Objective] This study aimed to optimize the extraction process parameters of Pu-erh tea polysaccharide. [Method] Single-factor experiment was carried out to analyze the influences of three main factors, including extraction temperature, ex- traction duration and solid-liquid ratio, on the extraction yield of tea polysaccharide. Box-Behnken central composite design and response surface methodology were adopted to determine the optimal extraction process of Pu-erh tea polysaccharide. [Result] The results of response surface analysis showed that the optimal extraction process was solid-liquid ratio of 1：17, extraction temperature of 80 ℃ and extraction duration of 78.5 min, and the Pu-erh tea polysaccharide yield was 12.72%. [Conclu- sion] Using response surface methodology （RSM） is feasible for the optimization of Pu-erh tea polysaccharide extraction process, and the tea polysaccharide yield increased significantly.展开更多
In commercial applications of phase Doppler anemometry (PDA), the effectiveness of non sphericity of particles is present and the response of PDA system deviates from the theoretical prediction. In this paper, the st...In commercial applications of phase Doppler anemometry (PDA), the effectiveness of non sphericity of particles is present and the response of PDA system deviates from the theoretical prediction. In this paper, the statistic characteristics of PDA signal related to irregular particles is analyzed and a method of statistic classification of irregular particles is proposed.It proves that the parameter of PDA signal for irregular particles is an unbiased estimation for spherical ones, the mean of the phase difference is in direct proportion to the mean diameter of particles and the standard deviation of the phase difference increases linearly with the standard deviation of irregular particles. As an application of the identification of irregular objects, fuzzy patterns and similarities of haemocytes are used to recognize and quantify cell samples.The statistic classification of particles is more significant in practice.展开更多
The one-order phase of the echo changes if there is relative radial moving between the object and the radar, i.e. , the Doppler effect. The Doppler effect is widely used in radar signal processing. The transverse moti...The one-order phase of the echo changes if there is relative radial moving between the object and the radar, i.e. , the Doppler effect. The Doppler effect is widely used in radar signal processing. The transverse motion of the object cannot change the one-order phase of the echo, but the high-order phase. The high-order Doppler effect of the transverse moving target is presented and a new algorithm for obtaining the transverse velocity is given. The scalar velocity of a target moving steadily in any direction can be calculated with one-order and two-order items of the echo phase. The calculating method and simulating results are given. As the transverse speed is 900 km/h, the speed calculation error is less than 0. 06% if SNR of echo signal is higher than 0 dB.展开更多
An experimental investigation associated with the basic fluid mechanics in an axial flow fan is described in this paper. The flow field in the tip region has been studied by laser Doppler anemometer (LDA) and flow vis...An experimental investigation associated with the basic fluid mechanics in an axial flow fan is described in this paper. The flow field in the tip region has been studied by laser Doppler anemometer (LDA) and flow visualization technique. Some experimental data and images are interpreted to understand the complex interactions between the annulus wall boundary layer and the leakage flow. It shows that the vortex inside the blade passage is produced by the separation of annulus wall boundary layer rather than the rolling up of leakage flow.展开更多
Objective Cervical spinal cord stimulation （SCS） has been found to augment cerebral blood flow （CBF） in a number of animal models. However, the effective use of SCS is hampered by a lack of understanding of its me...Objective Cervical spinal cord stimulation （SCS） has been found to augment cerebral blood flow （CBF） in a number of animal models. However, the effective use of SCS is hampered by a lack of understanding of its mechanism（s） of action. In this paper, we focus on the sympathetic and parasympathetic effects of SCS on CBF. Method SpragueDawley rats were selected for the experimental series. The animals were divided into 5 groups to underwent SCS and laser Doppler flowmeter （LDF） recordings. Control group, the animal underwent SCS and LDF recordings without any surgery of the nerve fibers and ganglia. V 1 group, the animal underwent bilateral resection of the nasociliary and post-ganglionic parasympathetic nerve fibbers. SCG group, the animal underwent bilateral resection of supper cervical ganglion. V 1 ＋ SCG group, the animal underwent both surgeries as V1- and SCG-group animals did. Sham group, the animal underwent the carotid manipulation with blunt-tipped forceps as well as the dissection of nasociliary and post-ganglionic parasympathetic nerve fibers around the ethmoidal foramen, but without cutting any nerves. Results During the SCS, the LDF was no statistical difference between the V 1 or SCG group and the control group. Yet, the effects of SCS on CBF are completely abolished in V1＋ SCG group. Conclusions Surgical interruption of both the parasympathetic and sympathetic pathways has the contradict effect on SCS-induced CBF augmentation.展开更多
The two-phase detection method for directional discrimination in laser Doppler measurements is discussed.The diffraction efficiency of a sin- gle period rectangular phase grating is analysed and a kind of back-scatter...The two-phase detection method for directional discrimination in laser Doppler measurements is discussed.The diffraction efficiency of a sin- gle period rectangular phase grating is analysed and a kind of back-scattered laser Doppler vibrometer without directional ambiguity using the single peri- od rectangular phase grating as the beam-combiner described.The principles of this kind of vibrometer are explained in detail,and some experimental re- sults are given.In this kind of vibrometer,the rectangular phase grating, without the zero diffracted order and even orders,is used to eliminate use- less stray light and to combine the useful signal light.Differential electronics is employed to reject signal noise.Therefore,the signal-to-noise ratio of Doppler signals and the measurement accuracy of the instrument are im- proved and the range of application is expanded.展开更多
[Objective] This study aimed to analyze the cause of the generation of short-term heavy precipitations in a regional heavy rainstorm in Shannxi Province. [Method] Taking a heavy rainstorm covering most parts of Shaanx...[Objective] This study aimed to analyze the cause of the generation of short-term heavy precipitations in a regional heavy rainstorm in Shannxi Province. [Method] Taking a heavy rainstorm covering most parts of Shaanxi Province in late July 2010 as an example, data of five Doppler weather radars in Shaanxi Province were employed for a detailed analysis of the evolution of the heavy rainstorm pro- cess. [Result] Besides the good large-scale weather background conditions, the de- velopment and evolution of some mesoscale and small-scale weather systems direct- ly led to short-term heavy precipitations during the heavy rainstorm process, involv- ing the intrusion of moderate IS-scale weak cold air and presence of small-scale wind shear, convergence and adverse wind area. In addition, small-scale convection echoes were arranged in lines and formed a ＂train effect＂, which would also con- tribute to the generation of short-term heavy precipitation. [Conclusion] This study provided basic information for more clear and in-depth analysis of the formation mechanism of short-term heavy precipitations.展开更多
During multi-pass conventional spinning, roller paths combined with the forward and the backward pass are usually used to improve the material formability. In order to understand the backward spinning process properly...During multi-pass conventional spinning, roller paths combined with the forward and the backward pass are usually used to improve the material formability. In order to understand the backward spinning process properly, the backward roller paths of hemispherical parts with aluminum alloy 2024-O are analyzed. Finite element model with parameterized conventional spinning roller paths, which are based on quadratic Bezier curves, is developed to explore the evolution of the stress, strain and thinning during the backward processes. Analysis of the simulation results reveals stress and strain features of backward pass spinning. According to the findings, the application of the backward pass can obviously improve the uniformity of wall thickness. Furthermore, references of the parameters in future backward path design are provided.展开更多
The captopril/Chitosan-gelatin net-polymer microspheres(CTP/CGNPMs) were prepared using Chitosan(CTS) and gelatin(GT) by the methods of emulsification,cross-linked reagent alone or in combination and microcrystalline ...The captopril/Chitosan-gelatin net-polymer microspheres(CTP/CGNPMs) were prepared using Chitosan(CTS) and gelatin(GT) by the methods of emulsification,cross-linked reagent alone or in combination and microcrystalline cellulose(MCC) added in the process of preparation of microspheres,which aimed to eliminate dose dumping and burst phenomenon of microspheres for the improvement of the therapeutic efficiency and the decrease of the side effects of captopril(CTP). The results indicated that CTP/CGNPMs had a spherical shape,smooth surface and integral structure inside but no adhesive phenomena in the preparation. The size distribution ranged from 220 μm to 280 μm. The CTP release test in vitro demonstrated that CTP/CGNPMs played the role of retarding the release of CTP compared with ordinary CTP tablets. The release behaviors of CGNPMS were influenced by preparation conditions such as experimental material ratio(EMR) and composition of cross linking reagents. Among these factors,the EMR(1/4),CLR(FA+SPP) and 0.75% microcrystalline cellulose(MCC) added to the microspheres constituted the optimal scheme for the preparation of CTP/CGNPMs. The ER,DL and SR of CTP/CGNPMs prepared according to the optimal scheme were 46.23±4.51%,9.95±0.77% and 261±42%,respectively. The CTP/CGNPMs had the good characteristics of sustained release of drug and the process of emulsification and cross-linking were simple and stable. The CGNPMs are likely to be an ideal sustained release formulation for water-soluble drugs.展开更多
基金Supported by National Key Technologies R & D Program(2007BAD58B03)~~
文摘[Objective] This study aimed to establish a pre-column derivatization HPLC method for the identification and analysis of monosaccharide composition of Pu-erh tea polysaccharide. [Method] Pu-erh tea polysaccharide was extracted using the wa- ter extraction method, further isolated and purified by DEAE cellulose-52 columns. The obtained tea polysaccharide and four components TPS1, TPS2, TPS3 and TPS, were first derived by 1-phenyl-3-methyl-5-pyrazolone （PMP）, and then the PMP derivatives of monosaccharide were analyzed by high performance liquid chromatog- raphy （HPLC）. [Result] Pu-erh tea polysaccharide contained eight kinds of monosac- chaddes （mannose, rhamnose, glucuronic acid, galacturonic acid, grucose, galactose, arabinose, fucose）, without xylose; so it was the same with TPS1; each of TPS2, TPS3 and TPS4 contained seven monosaccharides, while no fucose. [Conclusion] This method is simplified and rapid, which can be used to determine the monosac- charide composition of Pu-erh tea polysaccharide and monosaccharide content.
文摘Several Doppler shift estimators, including mean logarithm envelope difference (MLED) method, auto-correlation function (ACF) method, zero crossing rate (ZCR) method and mean square phase difference (MSPD) method were discussed and compared. The estimation principle and theoretical estimation bias of these estimators under Rayleigh fading channels were analyzed; furthermore, the Cramer Rao bound (CRB) of Doppler shift estimation was deduced, and a novel modification method based on two-dimensional polynomial fitting was proposed to reduce the Doppler shift estimation bias. We verified our algorithms with the Monte Carlo computer simulation; simulation results showed better variance performance of modified methods than those of the original methods. In addition, the applicable situations of these estimators were discussed.
基金supported by the Key Laboratory of Marine Hydrocarbon Resources and Environmental Geology(MRE201233)Key Laboratory of Surveying and Mapping Technology on Island and ReefState Bureau of Surveying and Mapping(2012B04)
文摘This paper analyzed the existing methods of wave measurement, and described the advantages of GPS applied in measuring the wave. The equations of absolute velocity estimation were discussed, focusing on two methods with Doppler shill. The error sources and their effect on velocity estimation were analyzed. Then, some tests were carried on to simulate dynamic velocity determination using static data Based on the high-frequency carrier-phase derived Doppler observations, the velocity has been estimated to the precision of 1 cm/s or so, even to the mm/s level. And with the receiver generated Doppler measurements, the precision can reach 3 - 15 cm/s.
基金Supported by National Science and Technology Support Program of China (2007BAD58B03)~~
文摘[Objective] This study aimed to optimize the extraction process parameters of Pu-erh tea polysaccharide. [Method] Single-factor experiment was carried out to analyze the influences of three main factors, including extraction temperature, ex- traction duration and solid-liquid ratio, on the extraction yield of tea polysaccharide. Box-Behnken central composite design and response surface methodology were adopted to determine the optimal extraction process of Pu-erh tea polysaccharide. [Result] The results of response surface analysis showed that the optimal extraction process was solid-liquid ratio of 1：17, extraction temperature of 80 ℃ and extraction duration of 78.5 min, and the Pu-erh tea polysaccharide yield was 12.72%. [Conclu- sion] Using response surface methodology （RSM） is feasible for the optimization of Pu-erh tea polysaccharide extraction process, and the tea polysaccharide yield increased significantly.
文摘In commercial applications of phase Doppler anemometry (PDA), the effectiveness of non sphericity of particles is present and the response of PDA system deviates from the theoretical prediction. In this paper, the statistic characteristics of PDA signal related to irregular particles is analyzed and a method of statistic classification of irregular particles is proposed.It proves that the parameter of PDA signal for irregular particles is an unbiased estimation for spherical ones, the mean of the phase difference is in direct proportion to the mean diameter of particles and the standard deviation of the phase difference increases linearly with the standard deviation of irregular particles. As an application of the identification of irregular objects, fuzzy patterns and similarities of haemocytes are used to recognize and quantify cell samples.The statistic classification of particles is more significant in practice.
基金Supported by the State Key Laboratory of Millimeter Waves(K200819)~~
文摘The one-order phase of the echo changes if there is relative radial moving between the object and the radar, i.e. , the Doppler effect. The Doppler effect is widely used in radar signal processing. The transverse motion of the object cannot change the one-order phase of the echo, but the high-order phase. The high-order Doppler effect of the transverse moving target is presented and a new algorithm for obtaining the transverse velocity is given. The scalar velocity of a target moving steadily in any direction can be calculated with one-order and two-order items of the echo phase. The calculating method and simulating results are given. As the transverse speed is 900 km/h, the speed calculation error is less than 0. 06% if SNR of echo signal is higher than 0 dB.
文摘An experimental investigation associated with the basic fluid mechanics in an axial flow fan is described in this paper. The flow field in the tip region has been studied by laser Doppler anemometer (LDA) and flow visualization technique. Some experimental data and images are interpreted to understand the complex interactions between the annulus wall boundary layer and the leakage flow. It shows that the vortex inside the blade passage is produced by the separation of annulus wall boundary layer rather than the rolling up of leakage flow.
文摘Objective Cervical spinal cord stimulation （SCS） has been found to augment cerebral blood flow （CBF） in a number of animal models. However, the effective use of SCS is hampered by a lack of understanding of its mechanism（s） of action. In this paper, we focus on the sympathetic and parasympathetic effects of SCS on CBF. Method SpragueDawley rats were selected for the experimental series. The animals were divided into 5 groups to underwent SCS and laser Doppler flowmeter （LDF） recordings. Control group, the animal underwent SCS and LDF recordings without any surgery of the nerve fibers and ganglia. V 1 group, the animal underwent bilateral resection of the nasociliary and post-ganglionic parasympathetic nerve fibbers. SCG group, the animal underwent bilateral resection of supper cervical ganglion. V 1 ＋ SCG group, the animal underwent both surgeries as V1- and SCG-group animals did. Sham group, the animal underwent the carotid manipulation with blunt-tipped forceps as well as the dissection of nasociliary and post-ganglionic parasympathetic nerve fibers around the ethmoidal foramen, but without cutting any nerves. Results During the SCS, the LDF was no statistical difference between the V 1 or SCG group and the control group. Yet, the effects of SCS on CBF are completely abolished in V1＋ SCG group. Conclusions Surgical interruption of both the parasympathetic and sympathetic pathways has the contradict effect on SCS-induced CBF augmentation.
文摘The two-phase detection method for directional discrimination in laser Doppler measurements is discussed.The diffraction efficiency of a sin- gle period rectangular phase grating is analysed and a kind of back-scattered laser Doppler vibrometer without directional ambiguity using the single peri- od rectangular phase grating as the beam-combiner described.The principles of this kind of vibrometer are explained in detail,and some experimental re- sults are given.In this kind of vibrometer,the rectangular phase grating, without the zero diffracted order and even orders,is used to eliminate use- less stray light and to combine the useful signal light.Differential electronics is employed to reject signal noise.Therefore,the signal-to-noise ratio of Doppler signals and the measurement accuracy of the instrument are im- proved and the range of application is expanded.
基金Supported by Special Fund for National Weather Service Forecaster of China (CMAYBY2011-050)~~
文摘[Objective] This study aimed to analyze the cause of the generation of short-term heavy precipitations in a regional heavy rainstorm in Shannxi Province. [Method] Taking a heavy rainstorm covering most parts of Shaanxi Province in late July 2010 as an example, data of five Doppler weather radars in Shaanxi Province were employed for a detailed analysis of the evolution of the heavy rainstorm pro- cess. [Result] Besides the good large-scale weather background conditions, the de- velopment and evolution of some mesoscale and small-scale weather systems direct- ly led to short-term heavy precipitations during the heavy rainstorm process, involv- ing the intrusion of moderate IS-scale weak cold air and presence of small-scale wind shear, convergence and adverse wind area. In addition, small-scale convection echoes were arranged in lines and formed a ＂train effect＂, which would also con- tribute to the generation of short-term heavy precipitation. [Conclusion] This study provided basic information for more clear and in-depth analysis of the formation mechanism of short-term heavy precipitations.
基金Project(2014CB046601)supported by the National Basic Research Program of ChinaProject(51675333)supported by the National Natural Science Foundation of China
文摘During multi-pass conventional spinning, roller paths combined with the forward and the backward pass are usually used to improve the material formability. In order to understand the backward spinning process properly, the backward roller paths of hemispherical parts with aluminum alloy 2024-O are analyzed. Finite element model with parameterized conventional spinning roller paths, which are based on quadratic Bezier curves, is developed to explore the evolution of the stress, strain and thinning during the backward processes. Analysis of the simulation results reveals stress and strain features of backward pass spinning. According to the findings, the application of the backward pass can obviously improve the uniformity of wall thickness. Furthermore, references of the parameters in future backward path design are provided.
文摘The captopril/Chitosan-gelatin net-polymer microspheres(CTP/CGNPMs) were prepared using Chitosan(CTS) and gelatin(GT) by the methods of emulsification,cross-linked reagent alone or in combination and microcrystalline cellulose(MCC) added in the process of preparation of microspheres,which aimed to eliminate dose dumping and burst phenomenon of microspheres for the improvement of the therapeutic efficiency and the decrease of the side effects of captopril(CTP). The results indicated that CTP/CGNPMs had a spherical shape,smooth surface and integral structure inside but no adhesive phenomena in the preparation. The size distribution ranged from 220 μm to 280 μm. The CTP release test in vitro demonstrated that CTP/CGNPMs played the role of retarding the release of CTP compared with ordinary CTP tablets. The release behaviors of CGNPMS were influenced by preparation conditions such as experimental material ratio(EMR) and composition of cross linking reagents. Among these factors,the EMR(1/4),CLR(FA+SPP) and 0.75% microcrystalline cellulose(MCC) added to the microspheres constituted the optimal scheme for the preparation of CTP/CGNPMs. The ER,DL and SR of CTP/CGNPMs prepared according to the optimal scheme were 46.23±4.51%,9.95±0.77% and 261±42%,respectively. The CTP/CGNPMs had the good characteristics of sustained release of drug and the process of emulsification and cross-linking were simple and stable. The CGNPMs are likely to be an ideal sustained release formulation for water-soluble drugs.
