Common or bread wheat ( Triticum aestivum L., AABBDD, 2n=42) originated ca. 8 000 years ago from hybridization of tetraploid wheat ( Triticum turgidum L., AABB, 2n=28) and diploid Aegilops tauschii Coss. (DD...Common or bread wheat ( Triticum aestivum L., AABBDD, 2n=42) originated ca. 8 000 years ago from hybridization of tetraploid wheat ( Triticum turgidum L., AABB, 2n=28) and diploid Aegilops tauschii Coss. (DD, 2n=14). An essential prerequisite for this evolutionary step is that the natural hybrids between tetraploid wheat and diploid Aegilops tauschii can produce relatively many filled seeds which germinated well. In this study, without special techniques, e.g. immature embryo culture, out of 22 Ae. tauschii accessions, the genotype AS60 produced relatively many filled seeds which germinated well. The seed germination percentages in the crosses of Ae. tauschii ×tetraploid wheat, tetraploid wheat× Ae. tauschii and Ae. tauschii ×common wheat were, respectively, 50.0%, 57.1% and 45.5%. It seems that Ae. tauschii accession AS60 has a unique genotype which facilitate hybrid seed development and viability, and which meets with the prerequisite for wheat evolutionary. Furthermore, the significance of this finding for common wheat improvement and evolution was discussed.展开更多
The genomic composition of 1911289, a wheat ( Tritium aestivum L.) maintainer of K-CMS, was examined by several methods, such as genomic in situ hybridization (GISH), biochemical marking, and DNA molecular marking. Th...The genomic composition of 1911289, a wheat ( Tritium aestivum L.) maintainer of K-CMS, was examined by several methods, such as genomic in situ hybridization (GISH), biochemical marking, and DNA molecular marking. The results got by GISH and PCR amplification of dispersed rye-specific repetitive DNA sequence suggested that the alien chromatin in T911289 derived from rye. Specifically PCR amplification of the rye-specific microsatellite primers (SCM9) and seed storage protein analysis indicated that the alien chromatin in T911289 had developed from the short arm of 1R chromosome of rye (1RS). PCR amplification by using microsatellite primers locating on 1BS and seed storage protein analysis also revealed that 1911289 had lost the arm of 1BS or a small distal segment of it. We conclude that T911289 is a heterogeneous population which displays two distinct different types of translocation, i.e. the Robertsonian translocation and small segment translocation. The Robertsonian translocation type observed in our study is different from the 1BL/1RS translocation which is widely used in wheat production; it may be a novel and complex translocation form. Though the linkage between the desirable agronomic traits and the deleterious genes expressed as sticky dough has not got broken in T911289, the recovery of small segment translocation will still benefit the genetic study of wheat and rye.展开更多
Recent work revealed that, in the genomes of polyploid wheat, there exists a class of low_copy and chromosome_specific sequences that are labile upon polyploid formation. This class of sequences was proposed to play ...Recent work revealed that, in the genomes of polyploid wheat, there exists a class of low_copy and chromosome_specific sequences that are labile upon polyploid formation. This class of sequences was proposed to play a critical role in the stabilization and establishment of nascent plant polyploids as new species. To further study this issue, five wheat chromosome 7B_specific sequences, isolated from common wheat (Triticum aestivum L.) by chromosome microdissection, were characterized. The sequences were studied by genomic Southern hybridizations on a collection of polyploid wheats and their diploid progenitors. Four sequences hybridized to all polyploid species, but at the diploid level to only species closely related to the B_genome of polyploid wheat. This indicates that these sequences originated with the divergence of the diploid species, and was then vertically transmitted to polyploids. One sequence hybridized to all species at both the diploid and polyploid levels, suggesting its elimination after the polyploid wheat formation. The hybridization of this sequence to two synthetic polyploid wheats indicated that sequence elimination is a rapid event and probably related to methylation status of the sequence. Based on the above results, we suggest that selective changes of low_copy sequences occur rapidly after polyploid formation, which may contribute to the differentiation of chromosomes in newly formed allopolyploid wheats.展开更多
Studies were carried out to establish an efficient regeneration system of three bread wheat cultivars. Results showed induction medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) had a higher plantlet regenerati...Studies were carried out to establish an efficient regeneration system of three bread wheat cultivars. Results showed induction medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) had a higher plantlet regeneration frequency than Piclorm, with an average frequency of 54% in all treatments. Optimal condition for different genotypic rice was as following: induction medium (MSS 3AA/2) with 0.5 mg L-1 2,4-D, regeneration medium (R) with 0.01 mg L-1 2,4-D and 3 mg L-1 KT. The average regeneration frequency reached 83.3% under the condition. Correlation analysis showed that root differentiation, in different level, correlated with green spot regeneration, and with the number of regenerated plants per callus. No correlation was found between green spots regenerated and the numbers of plants regenerated per callus.展开更多
[Objective] This study aimed to investigate the polymorphism of PBF en- coding genes from common wheat Chinese Spring (Triticum aestivum L.). [Method] Using common wheat Chinese Spring as the experimental material, ...[Objective] This study aimed to investigate the polymorphism of PBF en- coding genes from common wheat Chinese Spring (Triticum aestivum L.). [Method] Using common wheat Chinese Spring as the experimental material, gene-specific primers were designed and applied to amplify the genomic DNA of Chinese Spring. PCR products were isolated, purified and ligated into the cloning vector. Positive clones were randomly selected for sequencing. A series of softwares including DNAMAN, Signalp, PSIPRED, Nuc_PLoc and MEGA were employed for sequence assembly and alignment, signal peptide prediction, primary and secondary structure prediction, as well as analyses of subcellular location and phylogenetic relationships between the PBF family members in Poaceae. [Result] Twenty-five target sequences were obtained from the genome of hexaploid common wheat Chinese Spring, which were classified into three clusters based on the sequence similarity. SNPs exist at two loci of the subunit, resulting in the change of encoded amino acid residues and affecting the secondary structure of final product encoded. [Conclusion] PBF encoding sequences are extremely conservative in Chinese Spring with certain variations. This study provides theoretical reference to evaluate the expression efficiency of wheat storage proteins.展开更多
In order to marker dominant nuclear gene Ms2 with a blue grain, a 4E disomic addition line 'xiaoyanlanli'(2n=44, AABBDD+4EII) as the male parent to pollinate with male-sterile plants of durum wheat, controlled by...In order to marker dominant nuclear gene Ms2 with a blue grain, a 4E disomic addition line 'xiaoyanlanli'(2n=44, AABBDD+4EII) as the male parent to pollinate with male-sterile plants of durum wheat, controlled by a dominant nuclear gene Ms2, and a durum wheat line 89-2343 with Ms2 and blue seed marker on the same addition chromosome was developed. The genotype 89-2343 was crossed and backcrossed with a common wheat genotype 7739-3 to produce male fertile plants with blue seeds (MFP-BS). To combine the blue seed marker, dwarf male-sterile plants carrying RhtlO and Ms2 were fertilized by pollen from selected MFP-BS. At last, the combination of blue seed marker, Ms2 and RhtlO was successfully produced. The segregation ratio of male sterility, seed color as well as chromosome configurations of the combinations suggested that the blue seed marker, Ms2 and RhtlO were located on the same chromosome. Cytological analysis indicated that the male sterile wheat line with a blue seed marker was 43 in chromosome number, with an additional chromosome. The transmission rate for blue seed male-sterile plants was 22.1% in common. In addition, the potential value for blue marker sterile lines in wheat breeding and hybrid production is discussed.展开更多
The Danish 1801 census is available in a fully coded and standardised digital version. This makes it possible to analyse the living conditions of the Danes in new ways. A very central aspect of living conditions is ho...The Danish 1801 census is available in a fully coded and standardised digital version. This makes it possible to analyse the living conditions of the Danes in new ways. A very central aspect of living conditions is household and family. The census is divided by these lines and therefore calculations of household size and complexity are straight forward and this clearly gives one side of the picture. A totally different side is co-residence analysis. This is not straight forward, but because of the standardisation it is doable. By this means, you get a picture of the likelihood that a person of a given age group lives together with specific relatives. It gives an insight into a central part of living conditions and it gives a picture of differences between sexes and urbanisations. The paper brings the two ways of description together and shows that each has something to contribute to the picture of the Danes.展开更多
Studies on the effect of elevated CO2 on C dynamics in cultivated croplands are critical to a better understanding of the C cycling in response to climate change in agroecosystems. To evaluate the effects of elevated ...Studies on the effect of elevated CO2 on C dynamics in cultivated croplands are critical to a better understanding of the C cycling in response to climate change in agroecosystems. To evaluate the effects of elevated CO2 and different N fertilizer application levels on soil respiration, winter wheat (Triticum aestivum L. cv. Yangmai 14) plants were exposed to either ambient CO2 or elevated CO2 (ambient [CO2] + 200 μmol mol-1), under N fertilizer application levels of 112.5 and 225 kg N ha-1 (as low N and normal N subtreatments, respectively), for two growing seasons (2006-2007 and 2007-2008) in a rice-winter wheat rotation system typical in China. A split-plot design was adopted. A root exclusion method was used to partition soil respiration (RS) into heterotrophic respiration (RH) and autotrophic respiration (RA). Atmospheric CO2 enrichment increased seasonal cumulative RS by 11.8% at low N and 5.6% at normal N when averaged over two growing seasons. Elevated CO2 significantly enhanced (P 〈 0.05) RS (12.7%), mainly due to the increase in RH (caused by decomposition of larger amounts of rice residue under elevated CO2) during a relative dry season in 2007-2008. Higher N supply also enhanced RS under ambient and elevated CO2. In the 2007-2008 season, normal N treatment had a significant positive effect (P 〈 0.01) on seasonal cumulative RS relative to low N treatment when averaged across CO2 levels (16.3%). A significant increase in RA was mainly responsible for the enhanced RS under higher N supply. The correlation (r2) between RH and soil temperature was stronger (P 〈 0.001) than that between RS and soil temperature when averaged across all treatments in both seasons. Seasonal patterns of RA may be more closely related to the plant phenology than soil temperature. The Q10 (the multiplier to the respiration rate for a 10 ℃ increase in soil temperature) values of RS and RH were not affected by elevated CO2 or higher N supply. These results mainly suggested that the increase in RS at elevated CO2 depended on the input of rice residue, and the increase in RS at higher N supply was due to stimulated root growth and concomitant increase in RA during the wheat growing portion of a rice-winter wheat rotation system.展开更多
文摘Common or bread wheat ( Triticum aestivum L., AABBDD, 2n=42) originated ca. 8 000 years ago from hybridization of tetraploid wheat ( Triticum turgidum L., AABB, 2n=28) and diploid Aegilops tauschii Coss. (DD, 2n=14). An essential prerequisite for this evolutionary step is that the natural hybrids between tetraploid wheat and diploid Aegilops tauschii can produce relatively many filled seeds which germinated well. In this study, without special techniques, e.g. immature embryo culture, out of 22 Ae. tauschii accessions, the genotype AS60 produced relatively many filled seeds which germinated well. The seed germination percentages in the crosses of Ae. tauschii ×tetraploid wheat, tetraploid wheat× Ae. tauschii and Ae. tauschii ×common wheat were, respectively, 50.0%, 57.1% and 45.5%. It seems that Ae. tauschii accession AS60 has a unique genotype which facilitate hybrid seed development and viability, and which meets with the prerequisite for wheat evolutionary. Furthermore, the significance of this finding for common wheat improvement and evolution was discussed.
文摘The genomic composition of 1911289, a wheat ( Tritium aestivum L.) maintainer of K-CMS, was examined by several methods, such as genomic in situ hybridization (GISH), biochemical marking, and DNA molecular marking. The results got by GISH and PCR amplification of dispersed rye-specific repetitive DNA sequence suggested that the alien chromatin in T911289 derived from rye. Specifically PCR amplification of the rye-specific microsatellite primers (SCM9) and seed storage protein analysis indicated that the alien chromatin in T911289 had developed from the short arm of 1R chromosome of rye (1RS). PCR amplification by using microsatellite primers locating on 1BS and seed storage protein analysis also revealed that 1911289 had lost the arm of 1BS or a small distal segment of it. We conclude that T911289 is a heterogeneous population which displays two distinct different types of translocation, i.e. the Robertsonian translocation and small segment translocation. The Robertsonian translocation type observed in our study is different from the 1BL/1RS translocation which is widely used in wheat production; it may be a novel and complex translocation form. Though the linkage between the desirable agronomic traits and the deleterious genes expressed as sticky dough has not got broken in T911289, the recovery of small segment translocation will still benefit the genetic study of wheat and rye.
文摘Recent work revealed that, in the genomes of polyploid wheat, there exists a class of low_copy and chromosome_specific sequences that are labile upon polyploid formation. This class of sequences was proposed to play a critical role in the stabilization and establishment of nascent plant polyploids as new species. To further study this issue, five wheat chromosome 7B_specific sequences, isolated from common wheat (Triticum aestivum L.) by chromosome microdissection, were characterized. The sequences were studied by genomic Southern hybridizations on a collection of polyploid wheats and their diploid progenitors. Four sequences hybridized to all polyploid species, but at the diploid level to only species closely related to the B_genome of polyploid wheat. This indicates that these sequences originated with the divergence of the diploid species, and was then vertically transmitted to polyploids. One sequence hybridized to all species at both the diploid and polyploid levels, suggesting its elimination after the polyploid wheat formation. The hybridization of this sequence to two synthetic polyploid wheats indicated that sequence elimination is a rapid event and probably related to methylation status of the sequence. Based on the above results, we suggest that selective changes of low_copy sequences occur rapidly after polyploid formation, which may contribute to the differentiation of chromosomes in newly formed allopolyploid wheats.
文摘Studies were carried out to establish an efficient regeneration system of three bread wheat cultivars. Results showed induction medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) had a higher plantlet regeneration frequency than Piclorm, with an average frequency of 54% in all treatments. Optimal condition for different genotypic rice was as following: induction medium (MSS 3AA/2) with 0.5 mg L-1 2,4-D, regeneration medium (R) with 0.01 mg L-1 2,4-D and 3 mg L-1 KT. The average regeneration frequency reached 83.3% under the condition. Correlation analysis showed that root differentiation, in different level, correlated with green spot regeneration, and with the number of regenerated plants per callus. No correlation was found between green spots regenerated and the numbers of plants regenerated per callus.
基金Supported by National Natural Science Foundation of China(30900896)Special Fund for the Construction of Modern Agricultural Technology System(NYCYTX-001)Cyrus Tang Breeding Fund(A212020912)~~
文摘[Objective] This study aimed to investigate the polymorphism of PBF en- coding genes from common wheat Chinese Spring (Triticum aestivum L.). [Method] Using common wheat Chinese Spring as the experimental material, gene-specific primers were designed and applied to amplify the genomic DNA of Chinese Spring. PCR products were isolated, purified and ligated into the cloning vector. Positive clones were randomly selected for sequencing. A series of softwares including DNAMAN, Signalp, PSIPRED, Nuc_PLoc and MEGA were employed for sequence assembly and alignment, signal peptide prediction, primary and secondary structure prediction, as well as analyses of subcellular location and phylogenetic relationships between the PBF family members in Poaceae. [Result] Twenty-five target sequences were obtained from the genome of hexaploid common wheat Chinese Spring, which were classified into three clusters based on the sequence similarity. SNPs exist at two loci of the subunit, resulting in the change of encoded amino acid residues and affecting the secondary structure of final product encoded. [Conclusion] PBF encoding sequences are extremely conservative in Chinese Spring with certain variations. This study provides theoretical reference to evaluate the expression efficiency of wheat storage proteins.
文摘In order to marker dominant nuclear gene Ms2 with a blue grain, a 4E disomic addition line 'xiaoyanlanli'(2n=44, AABBDD+4EII) as the male parent to pollinate with male-sterile plants of durum wheat, controlled by a dominant nuclear gene Ms2, and a durum wheat line 89-2343 with Ms2 and blue seed marker on the same addition chromosome was developed. The genotype 89-2343 was crossed and backcrossed with a common wheat genotype 7739-3 to produce male fertile plants with blue seeds (MFP-BS). To combine the blue seed marker, dwarf male-sterile plants carrying RhtlO and Ms2 were fertilized by pollen from selected MFP-BS. At last, the combination of blue seed marker, Ms2 and RhtlO was successfully produced. The segregation ratio of male sterility, seed color as well as chromosome configurations of the combinations suggested that the blue seed marker, Ms2 and RhtlO were located on the same chromosome. Cytological analysis indicated that the male sterile wheat line with a blue seed marker was 43 in chromosome number, with an additional chromosome. The transmission rate for blue seed male-sterile plants was 22.1% in common. In addition, the potential value for blue marker sterile lines in wheat breeding and hybrid production is discussed.
文摘The Danish 1801 census is available in a fully coded and standardised digital version. This makes it possible to analyse the living conditions of the Danes in new ways. A very central aspect of living conditions is household and family. The census is divided by these lines and therefore calculations of household size and complexity are straight forward and this clearly gives one side of the picture. A totally different side is co-residence analysis. This is not straight forward, but because of the standardisation it is doable. By this means, you get a picture of the likelihood that a person of a given age group lives together with specific relatives. It gives an insight into a central part of living conditions and it gives a picture of differences between sexes and urbanisations. The paper brings the two ways of description together and shows that each has something to contribute to the picture of the Danes.
基金Supported by the National Natural Science Foundation of China(No.41171191)the National Key Technologies Research and Development Program of China during the 11th Five-Year Plan Period(No.2008BAD95B05)+1 种基金the Knowledge Innovation Program of the Chinese Academy of Sciences(Nos.KZCX2-YW-Q1-07,KZCX2-EW-409 and KZCX3-SW-440)the International Science and Technology Cooperation Program of China(No.2010DFA22770)
文摘Studies on the effect of elevated CO2 on C dynamics in cultivated croplands are critical to a better understanding of the C cycling in response to climate change in agroecosystems. To evaluate the effects of elevated CO2 and different N fertilizer application levels on soil respiration, winter wheat (Triticum aestivum L. cv. Yangmai 14) plants were exposed to either ambient CO2 or elevated CO2 (ambient [CO2] + 200 μmol mol-1), under N fertilizer application levels of 112.5 and 225 kg N ha-1 (as low N and normal N subtreatments, respectively), for two growing seasons (2006-2007 and 2007-2008) in a rice-winter wheat rotation system typical in China. A split-plot design was adopted. A root exclusion method was used to partition soil respiration (RS) into heterotrophic respiration (RH) and autotrophic respiration (RA). Atmospheric CO2 enrichment increased seasonal cumulative RS by 11.8% at low N and 5.6% at normal N when averaged over two growing seasons. Elevated CO2 significantly enhanced (P 〈 0.05) RS (12.7%), mainly due to the increase in RH (caused by decomposition of larger amounts of rice residue under elevated CO2) during a relative dry season in 2007-2008. Higher N supply also enhanced RS under ambient and elevated CO2. In the 2007-2008 season, normal N treatment had a significant positive effect (P 〈 0.01) on seasonal cumulative RS relative to low N treatment when averaged across CO2 levels (16.3%). A significant increase in RA was mainly responsible for the enhanced RS under higher N supply. The correlation (r2) between RH and soil temperature was stronger (P 〈 0.001) than that between RS and soil temperature when averaged across all treatments in both seasons. Seasonal patterns of RA may be more closely related to the plant phenology than soil temperature. The Q10 (the multiplier to the respiration rate for a 10 ℃ increase in soil temperature) values of RS and RH were not affected by elevated CO2 or higher N supply. These results mainly suggested that the increase in RS at elevated CO2 depended on the input of rice residue, and the increase in RS at higher N supply was due to stimulated root growth and concomitant increase in RA during the wheat growing portion of a rice-winter wheat rotation system.