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一种改进的丛枝菌根染色方法 被引量:46
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作者 杨亚宁 巴雷 +2 位作者 白晓楠 张立朝 王德利 《生态学报》 CAS CSCD 北大核心 2010年第3期774-779,共6页
研究改进了Vierheilig等描述的AM菌根染色法:将根样于20%KOH溶液中60℃水浴透明40-120 min,5%醋酸酸化5min后,用5%醋酸墨水染色液(派克纯黑书写墨水Quink),于60℃水浴染色30 min,清水浸泡脱色(14h)后即可镜检。根皮层细胞内AM真菌的丛... 研究改进了Vierheilig等描述的AM菌根染色法:将根样于20%KOH溶液中60℃水浴透明40-120 min,5%醋酸酸化5min后,用5%醋酸墨水染色液(派克纯黑书写墨水Quink),于60℃水浴染色30 min,清水浸泡脱色(14h)后即可镜检。根皮层细胞内AM真菌的丛枝结构清晰可见,并且能够明确地分辨AM真菌与其它未知真菌。此外,Quink初染后,再经过SudanⅣ复染(60℃、60 min),70%乙醇脱色5min,暗隔真菌的透明菌丝内所积聚的脂类颗粒被SudanⅣ染上鲜红色,在复式显微镜下能够观察到此类透明菌丝在根皮层组织内的存在状况。采用甘油明胶为封固剂制片,根的染色效果可以保存长久。此项技术可以对同一种植物的多个根样进行同步的透明和染色处理,而且操作简便、低毒性、成本低廉、染色效果极佳,适用于野生和栽培草本植物AM菌根的染色和制片观察。 展开更多
关键词 丛枝菌根 醋酸墨水 暗隔真菌
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Mycorrhizal colonization and distribution of arbuscular mycorrhizal fungi associated with Michelia champaca L.under plantation system in northeast India
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作者 Das Panna Kayang Highland 《Journal of Forestry Research》 SCIE CAS CSCD 2010年第2期137-142,I0003,I0004,共8页
Arbuscular mycorrhizal fungi(AMF) and dark septate endophyte(DSE) colonization were investigated in three different plantation sites(Umdihar,Umsaw and Mawlein) of Meghalaya,northeast India.Isolation and identifi... Arbuscular mycorrhizal fungi(AMF) and dark septate endophyte(DSE) colonization were investigated in three different plantation sites(Umdihar,Umsaw and Mawlein) of Meghalaya,northeast India.Isolation and identification of the AMF spore were conducted to evaluate the AMF diversity and host preference in terms of AMF species distribution and abundance in the plantation sites.Results showed that AMF colonization was significantly higher than dark septate endophyte colonization(p〉0.05).AMF and DSE colonization had a narrow range of colonization,varying from 50.91%-58.95% and 1.84%-4.11%,respectively.Spore density varied significantly in all the sites(p〉0.05).Out of 29 species identified from 7 genera,the species from Glomus was found to be highly abundant.Sorenson coefficient(Cs) ranged from 0.35-7.0.Species richness varied from 2.0-2.9 in the sites.Total species richness was significantly correlated with total relative abundance(p=0.001).The distribution,abundance and principal component analysis plot suggest that Glomus macrocarpum,G.multicaulis,G.constrictum and Acaulospora sp 1 were the most host preferred species which possibly may favour the host with proper nutrient acquisition and growth. 展开更多
关键词 arbuscular mycorrhizal colonization dark septate endophyte colonization GLOMUS Michelia champaca
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