An explosive epidemic disease of shrimp had occurred in China and the south Pacific coast. The causative agent is a new baculovirus called hypodermal and hematopoietic necrosis baculovirus (HHNBV) in China, and the pa...An explosive epidemic disease of shrimp had occurred in China and the south Pacific coast. The causative agent is a new baculovirus called hypodermal and hematopoietic necrosis baculovirus (HHNBV) in China, and the pathogen is very much similar to Japanese panaeid rod-shaped DNA virus (PRDV) which caused mass mortality of shrimp. In order to distinguish both HHNBV and PRDV at molecular level, we have sequenced the PCR-targeted DNA fragment of HHNBV. One pair of PCR primers were prepared based on the PRDV DNA. The sequence of the PCR-targeted DNA of HHNBV is 975bp in length, and shares 99.7% homology with the PRDV. The sequence of PCR-targeted DNA of HHNBV-XIA differs from PRDV DNA in deleting three nucleotides (CAT) at 510～512 site. These characteristics of the sequence classified HHNBV and PRDV as strains of different genotypes.展开更多
文摘An explosive epidemic disease of shrimp had occurred in China and the south Pacific coast. The causative agent is a new baculovirus called hypodermal and hematopoietic necrosis baculovirus (HHNBV) in China, and the pathogen is very much similar to Japanese panaeid rod-shaped DNA virus (PRDV) which caused mass mortality of shrimp. In order to distinguish both HHNBV and PRDV at molecular level, we have sequenced the PCR-targeted DNA fragment of HHNBV. One pair of PCR primers were prepared based on the PRDV DNA. The sequence of the PCR-targeted DNA of HHNBV is 975bp in length, and shares 99.7% homology with the PRDV. The sequence of PCR-targeted DNA of HHNBV-XIA differs from PRDV DNA in deleting three nucleotides (CAT) at 510～512 site. These characteristics of the sequence classified HHNBV and PRDV as strains of different genotypes.
