It is believed that whether the instantaneous objective function curves of plug-flow-reactor (PFR) and continuous-stirred-tank-reactor (CSTR) overlap or not, they have a consistent changing trend for complex reactions...It is believed that whether the instantaneous objective function curves of plug-flow-reactor (PFR) and continuous-stirred-tank-reactor (CSTR) overlap or not, they have a consistent changing trend for complex reactions (steady state, isothermal and constant volume). As a result of the relation of the objective functions (selectivity or yield) to the instantaneous objective functions (instantaneous selectivity or instantaneous reaction rate), the optimal reactor network configuration can be determined according to the changing trend of the instantaneous objective function curves. Further, a recent partition strategy for the reactor network synthesis based on the instantaneous objective function characteristic curves is proposed by extending the attainable region partition strategy from the concentration space to the instantaneous objective function-unreacted fraction of key reactant space. In this paper, the instantaneous objective function is closed to be the instantaneous selectivity and several samples are examined to illustrate the proposed method. The comparison with the previous work indicates it is a very convenient and practical systematic tool of the reactor network synthesis and seems also promising for overcoming the dimension limit of the attainable region partition strategy in the concentration space.展开更多
OBJECTIVE To explore the inhibition of ACHN cells via shRNAexpression vector mediated cyclinE1 gene silencing.METHODS The shRNA targeting at cyclinE1 gene was designedand synthesized. By ligation, the fragment was ins...OBJECTIVE To explore the inhibition of ACHN cells via shRNAexpression vector mediated cyclinE1 gene silencing.METHODS The shRNA targeting at cyclinE1 gene was designedand synthesized. By ligation, the fragment was inserted intopGenesil-1-U6 to construct the recombinant plasmid pGenesil-1-U6-cyclinE1. The identified recombinant plasmid was introducedinto ACHN cells with lipofectamine 2000. The inhibition ofcyclinE1 mRNA and protein expression were analyzed by RT-PCRand western-blotting. MTT method was used for observing cellproliferation and drawing growth curve. The cell cycle and ratiosof apoptotic cell were assessed by flow cytometric detection. Theability of invasion and speed of cell migration were detected bytranswell chamber invasive models and cell scratch method.RESULTS The inhibition of expression of cyclinE1 in ACHN cellsmediated by recombinant vector (0.0933 ± 0.05) was significantlylower than that in the group of transfected with empty vector(0.8827 ± 0.04) and the control group (0.9021 ± 0.03) (P < 0.05).Flow cytometry showed that recombinant cells were blocked inthe G_1 phase and the apoptotic ratio was increased significantly(11.15 ± 4.00)% (P < 0.05). The curves of cell growth indicated thatthe proliferation of cell transfected with recombinant plasmid wasinhibited significantly compared with that in control group (P <0.05). The results of transwell and cell scratch suggested that theabilities of invasion and migration of the cells transfected withrecombinant plasmid were decreased conspicuously (P < 0.05).CONCLUSION The expression of cyclinE1 could be inhibitedsuccessfully by RNA interference induced by shRNA expressionvector. This consequently inhibits the cell growth and inducesapoptosis. Our study provided a preliminary result in searching ofRNA interference (RNAi) therapy for renal cell carcinoma.展开更多
The flow rate measurements are the most difficult part of efficiency tests on prototype hydraulic turbines.Among the numerous flow rate measurement methods,the Winter Kennedy method is preferred for measuring turbine ...The flow rate measurements are the most difficult part of efficiency tests on prototype hydraulic turbines.Among the numerous flow rate measurement methods,the Winter Kennedy method is preferred for measuring turbine flow rates,since it is convenient,practical and economical.This paper describes efficiency tests on a large 300 MW Francis turbine,with the flow rate measured using the Winter Kennedy method and the Winter Kennedy flow rate coefficient calibrated using the Gibson method.The measured turbine efficiency curve is then compared with the curve provided by the manufacturer.The CFD calculations including the spiral case are then used to analyze the influence with the coefficient K and index n in the Winter Kennedy flow rate formula on the flow rate measurement.The uncertainty values of n and K are a key reason for the differences between the curves obtained from the efficiency test and the curves provided by the manufacturer.展开更多
The authors prove the space of harmonic functions with polynomial growth of a fixed rate on a complete noncompact Riemannian manifold with asymptotically nonnegative curvature is finite dimensional.
Experiments were conducted to investigate the cooling manner of an ultra-thick hot aluminum alloy plate during multistage quenching. Cooling curves and heat flux curves of different rapid quenching flux varied from 23...Experiments were conducted to investigate the cooling manner of an ultra-thick hot aluminum alloy plate during multistage quenching. Cooling curves and heat flux curves of different rapid quenching flux varied from 23 to 40 L min-1 and were analyzed in detail. In this investigation, cooling process was divided into the following four steps: (Ⅰ) starting step, (Ⅱ) rapid cooling step, (Ⅲ) slow cooling step, and (Ⅳ) stopping step. Based on the curves, the calculation method for surface transfer coefficient was provided, and the effects of coefficient on surface temperature and quenching flux were discussed. Results showed that the transfer coefficient disagreed with heat flux and that it is a nonlinear function of surface temperature. The highest coefficient was observed not in the rapid cooling step with the largest heat flux but in the slow cooling step with lower heat flux. The coefficient increased with surface temperature ranging from 480 to 150℃, and a coefficient peak appeared in the temperature range of 150- 100℃. The coefficient also increased with quenching flux. Finally, a simulation was performed using the finite element method to verify the reliability of the coefficient results, which showed good agreement with the measurement values.展开更多
The authors consider a family of smooth immersions F(., t) :M^n→R^n+1 of closed hypersurfaces in R^n+1 moving by the mean curvature flow F(p,t)/ t=-H(p,t).v(p,t) for t E [0, T). They show that if the norm...The authors consider a family of smooth immersions F(., t) :M^n→R^n+1 of closed hypersurfaces in R^n+1 moving by the mean curvature flow F(p,t)/ t=-H(p,t).v(p,t) for t E [0, T). They show that if the norm of the second fundamental form is bounded above by some power of mean curvature and the certain subcritical quantities concerning the mean curvature integral are bounded, then the flow can extend past time T. The result is similar to that in [6-9].展开更多
The operation parameters (vibration, shaft displacement and pressure fluctuation) of No. 1 Francis reversible unit of Baoquan pumped-storage power station were measured on site in the no-load mode at net heads of 51...The operation parameters (vibration, shaft displacement and pressure fluctuation) of No. 1 Francis reversible unit of Baoquan pumped-storage power station were measured on site in the no-load mode at net heads of 518.04, 522.01 and 530.38 m, re- spectively. The rotational speed fluctuations in the no-load mode at three net heads were beyond synchronization requirement with obvious S-shaped characteristic, and misaligned guide vanes (MGV) had to be put into use for synchronization. Further analysis demonstrated that the rotating frequency signal was generally dominant in vibration and shaft displacement mixing signal in the no-load mode, while the frequency domain was wide without an obvious main frequency in pressure fluctuation mixing signal. Besides, the SSTκ-ω turbulence model was adopted to simulate the four quadrant characteristic curves of Baoquan model pump-turbine at three gate openings, and the relative error between simulation results and model test data was within ±6%, indicating that the simulation method in this paper is feasible and S-shaped characteristic of the pump-turbine can be simulated with CFD method.展开更多
We systematically derive the Bianchi identities for the canonical connection on an almost Hermitian manifold.Moreover,we also compute the curvature tensor of the Levi-Civita connection on almost Hermitian manifolds in...We systematically derive the Bianchi identities for the canonical connection on an almost Hermitian manifold.Moreover,we also compute the curvature tensor of the Levi-Civita connection on almost Hermitian manifolds in terms of curvature and torsion of the canonical connection.As applications of the curvature identities,we obtain some results about the integrability of quasi K¨ahler manifolds and nearly K¨ahler manifolds.展开更多
In this paper, we establish the first variational formula and its Euler-Lagrange equation for the total 2p-th mean curvature functional .M2p of a submanifold Mn in a general Riemannian manifold gn^n+m for p = 0, 1,.....In this paper, we establish the first variational formula and its Euler-Lagrange equation for the total 2p-th mean curvature functional .M2p of a submanifold Mn in a general Riemannian manifold gn^n+m for p = 0, 1,..., [n/2]. As an example, we prove that closed complex submanifolds in complex projective spaces are critical points of the functional M2p, called relatively 2p-minimal submanifolds, for all p. At last, we discuss the relations between relatively 2p-minimal submanifoIds and austere submanifolds in real space forms, as well as a special variational problem.展开更多
A new concept of bend-mode inkjet nozzle with double PZT (lead zirconate titanate) actuators has been designed and fabricated in the present study. Then the pressure wave and fluid velocity at the nozzle exit have bee...A new concept of bend-mode inkjet nozzle with double PZT (lead zirconate titanate) actuators has been designed and fabricated in the present study. Then the pressure wave and fluid velocity at the nozzle exit have been investigated. The complex pressure behavior inside the channel was solved numerically based on the narrow channel acoustic theory. The two PZTs attached to a rectangular channel were actuated sequentially by setting the waveforms of each PZT to be center-aligned with various pulse widths. As a result, the double PZT actuation is superior to the single PZT actuation in view of strong momentum force and fast dissipation of residual pressure. The maximum fluid velocity at the nozzle exit is observed when the respective pulse widths equal to their optimum pulse widths. The numerical results are supported by the experimental results with the fabricated inkjet device by measuring the speed of meniscus just out of the nozzle.展开更多
All the possible equivalent barotropic (EB) laminar solutions are firstly explored,and all the possible non-EB elliptic circulations and hyperbolic laminar modes of rotating stratified fluids are discovered in this pa...All the possible equivalent barotropic (EB) laminar solutions are firstly explored,and all the possible non-EB elliptic circulations and hyperbolic laminar modes of rotating stratified fluids are discovered in this paper.The EB circulations (including the vortex streets and hurricane like vortices) possess rich structures,because either the arbitrary solutions of arbitrary nonlinear Poisson equations can be used or an arbitrary two-dimensional stream function is revealed which may be broadly applied in atmospheric and oceanic dynamics,plasma physics,astrophysics and so on.The discovery of the non-EB modes disproves a known conjecture.展开更多
基金Supported by the National Natural Science Foundation of China (No. 29776028, No. 29836140).
文摘It is believed that whether the instantaneous objective function curves of plug-flow-reactor (PFR) and continuous-stirred-tank-reactor (CSTR) overlap or not, they have a consistent changing trend for complex reactions (steady state, isothermal and constant volume). As a result of the relation of the objective functions (selectivity or yield) to the instantaneous objective functions (instantaneous selectivity or instantaneous reaction rate), the optimal reactor network configuration can be determined according to the changing trend of the instantaneous objective function curves. Further, a recent partition strategy for the reactor network synthesis based on the instantaneous objective function characteristic curves is proposed by extending the attainable region partition strategy from the concentration space to the instantaneous objective function-unreacted fraction of key reactant space. In this paper, the instantaneous objective function is closed to be the instantaneous selectivity and several samples are examined to illustrate the proposed method. The comparison with the previous work indicates it is a very convenient and practical systematic tool of the reactor network synthesis and seems also promising for overcoming the dimension limit of the attainable region partition strategy in the concentration space.
基金supported by a grant from Major State Basic Research Development Program,China(No.2002CB513107).
文摘OBJECTIVE To explore the inhibition of ACHN cells via shRNAexpression vector mediated cyclinE1 gene silencing.METHODS The shRNA targeting at cyclinE1 gene was designedand synthesized. By ligation, the fragment was inserted intopGenesil-1-U6 to construct the recombinant plasmid pGenesil-1-U6-cyclinE1. The identified recombinant plasmid was introducedinto ACHN cells with lipofectamine 2000. The inhibition ofcyclinE1 mRNA and protein expression were analyzed by RT-PCRand western-blotting. MTT method was used for observing cellproliferation and drawing growth curve. The cell cycle and ratiosof apoptotic cell were assessed by flow cytometric detection. Theability of invasion and speed of cell migration were detected bytranswell chamber invasive models and cell scratch method.RESULTS The inhibition of expression of cyclinE1 in ACHN cellsmediated by recombinant vector (0.0933 ± 0.05) was significantlylower than that in the group of transfected with empty vector(0.8827 ± 0.04) and the control group (0.9021 ± 0.03) (P < 0.05).Flow cytometry showed that recombinant cells were blocked inthe G_1 phase and the apoptotic ratio was increased significantly(11.15 ± 4.00)% (P < 0.05). The curves of cell growth indicated thatthe proliferation of cell transfected with recombinant plasmid wasinhibited significantly compared with that in control group (P <0.05). The results of transwell and cell scratch suggested that theabilities of invasion and migration of the cells transfected withrecombinant plasmid were decreased conspicuously (P < 0.05).CONCLUSION The expression of cyclinE1 could be inhibitedsuccessfully by RNA interference induced by shRNA expressionvector. This consequently inhibits the cell growth and inducesapoptosis. Our study provided a preliminary result in searching ofRNA interference (RNAi) therapy for renal cell carcinoma.
基金supported by the National Natural Science Foundation of China (Grant No.50979044)State Key Laboratory of Hydroscience and Engineering of China (Grant No. 2009T3)
文摘The flow rate measurements are the most difficult part of efficiency tests on prototype hydraulic turbines.Among the numerous flow rate measurement methods,the Winter Kennedy method is preferred for measuring turbine flow rates,since it is convenient,practical and economical.This paper describes efficiency tests on a large 300 MW Francis turbine,with the flow rate measured using the Winter Kennedy method and the Winter Kennedy flow rate coefficient calibrated using the Gibson method.The measured turbine efficiency curve is then compared with the curve provided by the manufacturer.The CFD calculations including the spiral case are then used to analyze the influence with the coefficient K and index n in the Winter Kennedy flow rate formula on the flow rate measurement.The uncertainty values of n and K are a key reason for the differences between the curves obtained from the efficiency test and the curves provided by the manufacturer.
基金Project supported by the National Natural Science Foundation of China (No.10271089).
文摘The authors prove the space of harmonic functions with polynomial growth of a fixed rate on a complete noncompact Riemannian manifold with asymptotically nonnegative curvature is finite dimensional.
基金supported by the National Basic Research Program of China(Grant No.2012CB619500)the Major State Research Program of China(Grant No.2016YFB0300901)+1 种基金the National Natural Science Foundation of China(Grant No.51375503)the BaGui Scholars Program of China’s Guangxi Zhuang Autonomous Region(Grant No.2013A017)
文摘Experiments were conducted to investigate the cooling manner of an ultra-thick hot aluminum alloy plate during multistage quenching. Cooling curves and heat flux curves of different rapid quenching flux varied from 23 to 40 L min-1 and were analyzed in detail. In this investigation, cooling process was divided into the following four steps: (Ⅰ) starting step, (Ⅱ) rapid cooling step, (Ⅲ) slow cooling step, and (Ⅳ) stopping step. Based on the curves, the calculation method for surface transfer coefficient was provided, and the effects of coefficient on surface temperature and quenching flux were discussed. Results showed that the transfer coefficient disagreed with heat flux and that it is a nonlinear function of surface temperature. The highest coefficient was observed not in the rapid cooling step with the largest heat flux but in the slow cooling step with lower heat flux. The coefficient increased with surface temperature ranging from 480 to 150℃, and a coefficient peak appeared in the temperature range of 150- 100℃. The coefficient also increased with quenching flux. Finally, a simulation was performed using the finite element method to verify the reliability of the coefficient results, which showed good agreement with the measurement values.
基金supported by the National Natural Science Foundation of China (Nos.10871069,10871070)the Shanghai Leading Academic Discipline Project (No.B407)
文摘The authors consider a family of smooth immersions F(., t) :M^n→R^n+1 of closed hypersurfaces in R^n+1 moving by the mean curvature flow F(p,t)/ t=-H(p,t).v(p,t) for t E [0, T). They show that if the norm of the second fundamental form is bounded above by some power of mean curvature and the certain subcritical quantities concerning the mean curvature integral are bounded, then the flow can extend past time T. The result is similar to that in [6-9].
文摘The operation parameters (vibration, shaft displacement and pressure fluctuation) of No. 1 Francis reversible unit of Baoquan pumped-storage power station were measured on site in the no-load mode at net heads of 518.04, 522.01 and 530.38 m, re- spectively. The rotational speed fluctuations in the no-load mode at three net heads were beyond synchronization requirement with obvious S-shaped characteristic, and misaligned guide vanes (MGV) had to be put into use for synchronization. Further analysis demonstrated that the rotating frequency signal was generally dominant in vibration and shaft displacement mixing signal in the no-load mode, while the frequency domain was wide without an obvious main frequency in pressure fluctuation mixing signal. Besides, the SSTκ-ω turbulence model was adopted to simulate the four quadrant characteristic curves of Baoquan model pump-turbine at three gate openings, and the relative error between simulation results and model test data was within ±6%, indicating that the simulation method in this paper is feasible and S-shaped characteristic of the pump-turbine can be simulated with CFD method.
基金supported by Science Foundation of Guangdong Province (Grant No. S2012010010038)National Natural Science Foundation of China (Grant No. 11571215)supporting project from the Department of Education of Guangdong Province (Grant No. Yq2013073)
文摘We systematically derive the Bianchi identities for the canonical connection on an almost Hermitian manifold.Moreover,we also compute the curvature tensor of the Levi-Civita connection on almost Hermitian manifolds in terms of curvature and torsion of the canonical connection.As applications of the curvature identities,we obtain some results about the integrability of quasi K¨ahler manifolds and nearly K¨ahler manifolds.
基金supported by National Natural Science Foundation of China(Grant No.11001016)the Specialized Research Fund for the Doctoral Program of Higher Education(Grant No. 20100003120003)the Program for Changjiang Scholars and Innovative Research Team in University
文摘In this paper, we establish the first variational formula and its Euler-Lagrange equation for the total 2p-th mean curvature functional .M2p of a submanifold Mn in a general Riemannian manifold gn^n+m for p = 0, 1,..., [n/2]. As an example, we prove that closed complex submanifolds in complex projective spaces are critical points of the functional M2p, called relatively 2p-minimal submanifolds, for all p. At last, we discuss the relations between relatively 2p-minimal submanifoIds and austere submanifolds in real space forms, as well as a special variational problem.
基金supported by Basic Science Research Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Education,Science and Technology(2010-0009054)
文摘A new concept of bend-mode inkjet nozzle with double PZT (lead zirconate titanate) actuators has been designed and fabricated in the present study. Then the pressure wave and fluid velocity at the nozzle exit have been investigated. The complex pressure behavior inside the channel was solved numerically based on the narrow channel acoustic theory. The two PZTs attached to a rectangular channel were actuated sequentially by setting the waveforms of each PZT to be center-aligned with various pulse widths. As a result, the double PZT actuation is superior to the single PZT actuation in view of strong momentum force and fast dissipation of residual pressure. The maximum fluid velocity at the nozzle exit is observed when the respective pulse widths equal to their optimum pulse widths. The numerical results are supported by the experimental results with the fabricated inkjet device by measuring the speed of meniscus just out of the nozzle.
基金Project supported by the National Natural Science Foundation of China (Nos.11175092,10735030)the National Basic Research Program of China (973 Program) (No.2007CB814800)+1 种基金the Natural Science Foundation of Shanghai (No.09ZR1413600)the K.C.Wong Magna Fund of Ningbo University
文摘All the possible equivalent barotropic (EB) laminar solutions are firstly explored,and all the possible non-EB elliptic circulations and hyperbolic laminar modes of rotating stratified fluids are discovered in this paper.The EB circulations (including the vortex streets and hurricane like vortices) possess rich structures,because either the arbitrary solutions of arbitrary nonlinear Poisson equations can be used or an arbitrary two-dimensional stream function is revealed which may be broadly applied in atmospheric and oceanic dynamics,plasma physics,astrophysics and so on.The discovery of the non-EB modes disproves a known conjecture.