Based on the nonlinear Barton–Bandis(B–B)failure criterion,this study considers the system reliability of rock wedge stability under the pseudo-static seismic load.The failure probability(Pf)of the system is calcula...Based on the nonlinear Barton–Bandis(B–B)failure criterion,this study considers the system reliability of rock wedge stability under the pseudo-static seismic load.The failure probability(Pf)of the system is calculated based on the Monte−Carlo method when considering parameter correlation and variability.Parameter analysis and sensitivity analysis are carried out to explore the influence of parameters on reliability.The relationships among the failure probability,safety factor(Fs),and variation coefficient are explored,and then stability probability curves of the rock wedge under the pseudo-static seismic load are drawn.The results show that the parameter correlation of the B–B failure criterion has a significant influence on the failure probability,but correlation increases system reliability or decreases system reliability affected by other parameters.Under the pseudo-static seismic action,sliding on both planes is the main failure mode of wedge system.In addition,the parameters with relatively high sensitivity are two angles related to the joint dip.When the coefficient of variation is consistent,the probability of system failure is a function of the safety factor.展开更多
Targeting Induced Local Lesions IN Genomes (TILLING) is a reverse genetics strategy for the high-throughput screening of induced mutations.γ, radiation, which often induces both insertion/deletion (Indel) and poi...Targeting Induced Local Lesions IN Genomes (TILLING) is a reverse genetics strategy for the high-throughput screening of induced mutations.γ, radiation, which often induces both insertion/deletion (Indel) and point mutations, has been widely used in mutation induction and crop breeding. The present study aimed to develop a simple, high-throughput TILLING system for screening γ ray-induced mutations using high-resolution melting (HRM) analysis. Pooled rice (Oryza sativa) samples mixed at a 1:7 ratio of Indel mutant to wild-type DNA could be distinguished from the wild-type controls by HRM analysis. Thus, an HRM-TILLING system that analyzes pooled samples of four M2 plants is recommended for screening γ, ray-induced mutants in rice. For demonstration, a γ, ray-mutagenized M2 rice population (n=4560) was screened for mutations in two genes, OsLCT1 and SPDT, using this HRM-TILLING system. Mutations including one single nucleotide substitution (G→A) and one single nucleotide insertion (A) were identified in OsLCT1, and one tdnucleotide (TTC) deletion was identified in SPDT. These mutants can be used in rice breeding and genetic studies, and the findings are of importance for the application of γ, ray mutagenesis to the breeding of rice and other seed crops.展开更多
We formulate a class of functionals in space forms such that its critical points include the r-minimal hyper-surface and the minimal hyper-surface as special cases. We obtain the algebraic, differential and variationa...We formulate a class of functionals in space forms such that its critical points include the r-minimal hyper-surface and the minimal hyper-surface as special cases. We obtain the algebraic, differential and variational characteristics of the critical surfaces determined by the critical points. We prove the Simons' type nonexistence theorem which indicates that in the unit sphere, there exists no stable critical surfaces, and the Alexandrov's type existence theorem which indicates that in Euclidean space, the sphere is the only stable critical surfaces.展开更多
In this paper, the relationship between the existence of closed geodesics and the volume growth of complete noncompact Riemannian manifolds is studied. First the authors prove a diffeomorphic result of such an n-m2nif...In this paper, the relationship between the existence of closed geodesics and the volume growth of complete noncompact Riemannian manifolds is studied. First the authors prove a diffeomorphic result of such an n-m2nifold with nonnegative sectional curvature, which improves Marenich-Toponogov's theorem. As an application, a rigidity theorem is obtained for nonnegatively curved open manifold which contains a clesed geodesic. Next the authors prove a theorem about the nonexistence of closed geodesics for Riemannian manifolds with sectional curvature bounded from below by a negative constant.展开更多
基金Project(51878668)supported by the National Natural Science Foundation of ChinaProjects(2017-122-058,2018-123-040)supported by the Guizhou Provincial Department of Transportation Foundation,ChinaProject([2018]2815)supported by the Guizhou Provincial Department of Science and Technology Foundation,China。
文摘Based on the nonlinear Barton–Bandis(B–B)failure criterion,this study considers the system reliability of rock wedge stability under the pseudo-static seismic load.The failure probability(Pf)of the system is calculated based on the Monte−Carlo method when considering parameter correlation and variability.Parameter analysis and sensitivity analysis are carried out to explore the influence of parameters on reliability.The relationships among the failure probability,safety factor(Fs),and variation coefficient are explored,and then stability probability curves of the rock wedge under the pseudo-static seismic load are drawn.The results show that the parameter correlation of the B–B failure criterion has a significant influence on the failure probability,but correlation increases system reliability or decreases system reliability affected by other parameters.Under the pseudo-static seismic action,sliding on both planes is the main failure mode of wedge system.In addition,the parameters with relatively high sensitivity are two angles related to the joint dip.When the coefficient of variation is consistent,the probability of system failure is a function of the safety factor.
基金Project supported by the National Key Research and Development Program of China(No.2016YFD0102103)
文摘Targeting Induced Local Lesions IN Genomes (TILLING) is a reverse genetics strategy for the high-throughput screening of induced mutations.γ, radiation, which often induces both insertion/deletion (Indel) and point mutations, has been widely used in mutation induction and crop breeding. The present study aimed to develop a simple, high-throughput TILLING system for screening γ ray-induced mutations using high-resolution melting (HRM) analysis. Pooled rice (Oryza sativa) samples mixed at a 1:7 ratio of Indel mutant to wild-type DNA could be distinguished from the wild-type controls by HRM analysis. Thus, an HRM-TILLING system that analyzes pooled samples of four M2 plants is recommended for screening γ, ray-induced mutants in rice. For demonstration, a γ, ray-mutagenized M2 rice population (n=4560) was screened for mutations in two genes, OsLCT1 and SPDT, using this HRM-TILLING system. Mutations including one single nucleotide substitution (G→A) and one single nucleotide insertion (A) were identified in OsLCT1, and one tdnucleotide (TTC) deletion was identified in SPDT. These mutants can be used in rice breeding and genetic studies, and the findings are of importance for the application of γ, ray mutagenesis to the breeding of rice and other seed crops.
基金supported by National Natural Science Foundation of China (Grant No.10871061)
文摘We formulate a class of functionals in space forms such that its critical points include the r-minimal hyper-surface and the minimal hyper-surface as special cases. We obtain the algebraic, differential and variational characteristics of the critical surfaces determined by the critical points. We prove the Simons' type nonexistence theorem which indicates that in the unit sphere, there exists no stable critical surfaces, and the Alexandrov's type existence theorem which indicates that in Euclidean space, the sphere is the only stable critical surfaces.
基金Project supported by the National Natural Science Foundation of China(Nos.10971055,11171096)the Research Fund for the Doctoral Program of Higher Education of China(No.20104208110002)the Funds for Disciplines Leaders of Wuhan(No.Z201051730002)
文摘In this paper, the relationship between the existence of closed geodesics and the volume growth of complete noncompact Riemannian manifolds is studied. First the authors prove a diffeomorphic result of such an n-m2nifold with nonnegative sectional curvature, which improves Marenich-Toponogov's theorem. As an application, a rigidity theorem is obtained for nonnegatively curved open manifold which contains a clesed geodesic. Next the authors prove a theorem about the nonexistence of closed geodesics for Riemannian manifolds with sectional curvature bounded from below by a negative constant.