高效液相色谱法测定曼陀罗叶中东莨菪碱和阿托品的含量

建立HPLC法同时测定曼陀罗叶中东莨菪碱和阿托品含量的方法。采用waters XTerra RP18(4.6×150μm,5μm)色谱柱,以乙腈-水(含0.035 mol/L磷酸钠和0.0087 moL/L的十二烷基硫酸钠,0.5%磷酸,0.15%三乙胺)(35∶65)为流动相,检测波长为216 nm,流速为1 mL/min,柱温为30℃,进样量各10μL,以氢溴酸东莨菪碱和硫酸阿托品测定曼陀罗叶中东莨菪碱和阿托品含量。结果表明:氢溴酸东莨菪碱和硫酸阿托品进样浓度分别在0.010~0.206 mg/mL(r=0.999,n=5)和0.041~0.818 mg·mL^(-1)(r=0.999,n=5)范围内线性关系良好,平均回收率分别为97.4%和98.4%。经方法学验证,该法操作简单、重复性好、准确度高,可以作为曼陀罗叶中东莨菪碱和阿托品含量测定的方法。

不同叶形曼陀罗叶结构比较及鉴别方法研究

目的:建立曼陀罗叶结构分析及性状显微鉴别方法,为进一步完善质量标准提供基础。方法:依据叶结构分析方法对曼陀罗叶特征规范描述量化对比,同时使用植物形态学和性状显微鉴别的方法对其形态比对分析研究。结果:曼陀罗叶叶片形态特征多变;主要表现在叶形、叶主脉两侧同编号裂片形态及叶基部形态;显微特征主要表现在叶草酸钙结晶晶型,叶主脉维管束的形态,导管排列和孔径的差异。结论:研究结果为规范药材质量标准用语、曼陀罗叶鉴别及质量标准修订提高提供参考。

DPPH法评价分析海南曼陀罗果叶抗氧化活性

采用DPPH自由基法对海南曼陀罗果和曼陀罗叶的抗氧化活性进行对比研究,研究表明:曼陀罗提取物果的抗氧化活性大于曼陀罗叶。曼陀罗果各萃取段DPPH自由基清除率强弱依次为氯仿段﹥乙酸乙酯段﹥石油醚段,曼陀罗叶各萃取段DPPH自由基清除率强弱依次为乙酸乙酯段﹥氯仿段﹥石油醚段,且曼陀罗叶乙酸乙酯萃取段的清除DPPH自由基的IC50高达0.145mg/ml,只比BHT的IC50略低。

DPPH法评价分析海南曼陀罗果叶抗氧化活性

采用DPPH自由基法对海南曼陀罗果和曼陀罗叶的抗氧化活性进行对比研究,研究表明:曼陀罗提取物果的抗氧化活性大于曼陀罗叶。曼陀罗果各萃取段DPPH自由基清除率强弱依次为氯仿段﹥乙酸乙酯段﹥石油醚段,曼陀罗叶各萃取段DPPH自由基清除率强弱依次为乙酸乙酯段﹥氯仿段﹥石油醚段,且曼陀罗叶乙酸乙酯萃取段的清除DPPH自由基的IC50高达0.145mg/ml,只比BHT的IC50略低。

Study on Adaptability of Datura stramonium and Datura stramonium var. tatual to Temperature

This study was conducted to investigate the temperature adaptability of Datura stramonium and D. stramonium var. tatual. Under 4 temperature levels,plantgrowth and pigment concentrations in leaves and stems were determined, and leaf anatomic structures of D. stramonium var. tatual and D. stramonium were analyzed. The results showed that the palisade tissue thickness/sponge tissue thickness,stomatal density and stomatal index values of D. stramonium var. tatual were significantly greater than D. stramonium; the chlorophyll content/carotenoid content in leaves of D. stramonium var. tatual increased at first and decreased then, while no regularity was found in leaves of D. stramonium; and the contents of anthocyanin,flavonoids, and total phenols in stems of D. stramonium var. tatual were significantly higher than those in stems of D. stramonium. The temperature adaptability of D.stramonium var. tatual is stronger than that of D. stramonium.

日本筛选天然抗癌药

日本东京医科大学从1979年起便致力于抗癌药的筛选。方法是以热水、乙醇、甲醇提取液,按100mg/kg(体重)给予荷瘤(S180腹水型)雄性小鼠,测定抑制率,结果筛选出的天然药物抑制率在50％以上的有万年青、败酱草、牛膝、石苇、日本拔葜、菊花、地榆、香菇、水杨梅、山姜、龙胆、良姜、桑自皮、蜈蚣、曼陀罗叶、泽兰、苍耳子,60％以上的有红柳皮、半夏、虎杖根、