纸层析-分光光度法测定发酵液中L-异亮氨酸

鉴于在菌种选育工作中必须准确测定菌株产酸水平,要求有可行的测定方法,对简单易行的纸层析 分光光度法测定发酵液中L 异亮氨酸质量进行了系统的研究,研究发现:选用合适的显色剂、层析溶剂及洗脱剂的同时选择最佳吸收波长、最佳洗脱时间及适宜的点样量,可以提高该测定方法的准确度和精密度,从而在菌种筛选过程中准确地测定发酵液中L 异亮氨酸质量.

PDS脱硫液中多硫化物的测定方法

对PDS脱硫液中多硫化物的测定进行了研究,通过紫外分光光度法测定脱硫液中多硫化物的含量。分析脱硫液中不同物质的光谱图,得到了多硫化物的最佳吸收波长是在285nm处,研究了多硫化钠的稳定性、pH值对标准曲线的影响及在多硫化物稀释过程中稀释液的pH值对测定的影响,得到了多硫化物测定的标准曲线,通过加标测量的方法,证明了测定方法的准确性。

Spectrophotometric determination of heparin with safranine T and their interaction mechanism

In weak acidic medium the alkaline cationic Safranine T interacts with acidic biological rnacromolecule heparin intensively causing the change of the molecular conformation, the maximum absorption wavelength and the absorption value. The optimum experimental condition was given and a new method to determine the heparin was established. The linear range was 0.05-2.0 mg/L and the correlation coefficient was 0.9974. The method has been applied to analyze the samples and the results are satisfactory. Furthermore, the interaction mechanism was indicated.

铬天青-S分光光度法测定水中铝含量方法改进

改进水中铝的测定条件结果,改进后铝的浓度在0.05ug/ml～0.25ug/ml范围内,其标准系列线性良好,相关系数r=0.9998,结论:改进铝的测定方法,操作简单、快速、保障测定结果的准确度。