Streptococcus iniae has been detected in several regions in Indonesia, such as Bali, Jambi and Papua. Outbreak of meningoencephalitis and septicemia was still often found in Papua. The aim of study was to compare phen...Streptococcus iniae has been detected in several regions in Indonesia, such as Bali, Jambi and Papua. Outbreak of meningoencephalitis and septicemia was still often found in Papua. The aim of study was to compare phenotype, genotype and histopathology of S. iniae from Papua. Bacteria was identified using morphological and biochemical tests. It was also extracted, amplified and sequenced to see genotypic characters. The primer used were 5＇-AGAGTTTGATCCTGGCT-3＇ （24F） and 5＇-AAGGGAGGTGATCCAGCCGCA-3＇ （1540R） in 16S rRNA region. Result of sequencing was analyzed by the neighbor joining and maximum parsimony methods. The identification result of isolate from Papua was S. iniae. The amplification result was a sharp band--l,500 bp band in 16S rRNA region. The phylogenetic tree showed that isolate from Papua was closely related to S. iniae strains CMS005 from Guangxi China. Bacterial clumps of S. iniae was firstly found in the blood vessel of liver at the 3rd day after infection and then caused the inflammation to spleen, heart, brain, kidney and gut at the 7th day post infection.展开更多
文摘Streptococcus iniae has been detected in several regions in Indonesia, such as Bali, Jambi and Papua. Outbreak of meningoencephalitis and septicemia was still often found in Papua. The aim of study was to compare phenotype, genotype and histopathology of S. iniae from Papua. Bacteria was identified using morphological and biochemical tests. It was also extracted, amplified and sequenced to see genotypic characters. The primer used were 5＇-AGAGTTTGATCCTGGCT-3＇ （24F） and 5＇-AAGGGAGGTGATCCAGCCGCA-3＇ （1540R） in 16S rRNA region. Result of sequencing was analyzed by the neighbor joining and maximum parsimony methods. The identification result of isolate from Papua was S. iniae. The amplification result was a sharp band--l,500 bp band in 16S rRNA region. The phylogenetic tree showed that isolate from Papua was closely related to S. iniae strains CMS005 from Guangxi China. Bacterial clumps of S. iniae was firstly found in the blood vessel of liver at the 3rd day after infection and then caused the inflammation to spleen, heart, brain, kidney and gut at the 7th day post infection.
