服生理盐水组大鼠舌粘膜上皮及空肠隐窝细胞有丝分裂指数(MI.长春新碱阻止法)均呈现近似昼夜节律变(峰值在卯时,谷值在酉时),小鼠回肠上皮 MI 有早(高)、晚(低)波动。用大黄、番泻叶加利血平所致的实验性脾虚动物组有明显不同的节律性...服生理盐水组大鼠舌粘膜上皮及空肠隐窝细胞有丝分裂指数(MI.长春新碱阻止法)均呈现近似昼夜节律变(峰值在卯时,谷值在酉时),小鼠回肠上皮 MI 有早(高)、晚(低)波动。用大黄、番泻叶加利血平所致的实验性脾虚动物组有明显不同的节律性改变。四君子汤能使脾虚大鼠舌粘膜细胞 MI 节律性较为正常,而对小肠 MI 节律性的恢复作用较弱。四君子汤有使脾虚动物中 MI 低下的提升到接近正常水平的效果。展开更多
本文检验了40例肾虚病人外周血 NK 细胞活性以及单个核细胞产生 IL—2和 IL—2受体表达能力,结果:肾虚病人 NK 活性、IL—2和 IL—2受体活性均较正常对照显著降低(P<0.01);正常对照 NK 活性与 IL—2活性呈直线正相关,而肾虚病人无明...本文检验了40例肾虚病人外周血 NK 细胞活性以及单个核细胞产生 IL—2和 IL—2受体表达能力,结果:肾虚病人 NK 活性、IL—2和 IL—2受体活性均较正常对照显著降低(P<0.01);正常对照 NK 活性与 IL—2活性呈直线正相关,而肾虚病人无明显相关性。展开更多
Objective： The aim of the study was to investigate the mechanism of gemcitabine （GEM） combination with radiation on the high metastasis human ovarian cancer cell line （HO-8910PM）. Methods： Human ovarian cancer c...Objective： The aim of the study was to investigate the mechanism of gemcitabine （GEM） combination with radiation on the high metastasis human ovarian cancer cell line （HO-8910PM）. Methods： Human ovarian cancer cell line HO- 8910PM was treated with different concentrations of gemcitabine for 24 h, then the cells were counted. In the study of GEM combination with radiation, an efficiency of colony formation was observed; the cell cycle and apoptosis were analyzed by flow cytometry; the experiment of depend on the time and its radio sensitivity were observed by using mitotic index with the cells for each 24, 48, 72 and 96 h after experiment. Results： It suggested that the GEM had an inhibition effect on the human ovarian cancer cell line. The alive cell numbers were decreased by following a height of GEM concentration. When GEM in combina- tion with a radiation, the suppression was significantly increased than that of single GEM therapy. The efficiency of colony formation was significantly lower, under this condition the cell could be arrested at G0-G1 phase and could be decreased to enter into the S phase; the apoptosis percentage could be significantly increased; especially, under the 4 Gy and 6 Gy doses the cell apoptosis was more obvious. GEM combination with radiation had depended on the time to the cells; mitotic index of the calls in combination group was observed significantly lower than that of single GEM therapy or single radiation, and this showed that it had an effect of radiosensitivity. Conclusion： The GEM has a significant growth inhibition on the human ovarian cancer cells, GEM combination with radiation could induce HO-8910PM cell occurred arrested and apoptosis. It has depended on the time and has a radiosensitivity effect. The result shows that it is a better method to treat the human ovarian cancer by using radiotherapy combined with gemcitabine.展开更多
文摘服生理盐水组大鼠舌粘膜上皮及空肠隐窝细胞有丝分裂指数(MI.长春新碱阻止法)均呈现近似昼夜节律变(峰值在卯时,谷值在酉时),小鼠回肠上皮 MI 有早(高)、晚(低)波动。用大黄、番泻叶加利血平所致的实验性脾虚动物组有明显不同的节律性改变。四君子汤能使脾虚大鼠舌粘膜细胞 MI 节律性较为正常,而对小肠 MI 节律性的恢复作用较弱。四君子汤有使脾虚动物中 MI 低下的提升到接近正常水平的效果。
文摘Objective： The aim of the study was to investigate the mechanism of gemcitabine （GEM） combination with radiation on the high metastasis human ovarian cancer cell line （HO-8910PM）. Methods： Human ovarian cancer cell line HO- 8910PM was treated with different concentrations of gemcitabine for 24 h, then the cells were counted. In the study of GEM combination with radiation, an efficiency of colony formation was observed; the cell cycle and apoptosis were analyzed by flow cytometry; the experiment of depend on the time and its radio sensitivity were observed by using mitotic index with the cells for each 24, 48, 72 and 96 h after experiment. Results： It suggested that the GEM had an inhibition effect on the human ovarian cancer cell line. The alive cell numbers were decreased by following a height of GEM concentration. When GEM in combina- tion with a radiation, the suppression was significantly increased than that of single GEM therapy. The efficiency of colony formation was significantly lower, under this condition the cell could be arrested at G0-G1 phase and could be decreased to enter into the S phase; the apoptosis percentage could be significantly increased; especially, under the 4 Gy and 6 Gy doses the cell apoptosis was more obvious. GEM combination with radiation had depended on the time to the cells; mitotic index of the calls in combination group was observed significantly lower than that of single GEM therapy or single radiation, and this showed that it had an effect of radiosensitivity. Conclusion： The GEM has a significant growth inhibition on the human ovarian cancer cells, GEM combination with radiation could induce HO-8910PM cell occurred arrested and apoptosis. It has depended on the time and has a radiosensitivity effect. The result shows that it is a better method to treat the human ovarian cancer by using radiotherapy combined with gemcitabine.
