目的:探讨抑癌基因p57k ip2和细胞周期素E(cyc linE)在宫颈癌发生发展中的作用及其与临床病理及预后的意义.方法:采用免疫组化二步法检测p57k ip2和cyc lin E在48例宫颈癌、13例宫颈上皮内瘤变(C IN)和15例正常宫颈组织中的表达.结果:p5...目的:探讨抑癌基因p57k ip2和细胞周期素E(cyc linE)在宫颈癌发生发展中的作用及其与临床病理及预后的意义.方法:采用免疫组化二步法检测p57k ip2和cyc lin E在48例宫颈癌、13例宫颈上皮内瘤变(C IN)和15例正常宫颈组织中的表达.结果:p57k ip2在宫颈癌中的阳性表达率为21%,低于正常组(93%)和C IN组(77%),差异有显著性意义(P<0.01).cyc lin E在宫颈癌中的阳性表达率为77%,明显高于正常组(13%),差异有显著性意义(P<0.01).在宫颈癌组织中p57k ip2和cyc lin E的表达呈负相关(r=-0.597,P<0.01).p57k ip2的表达与病理分化程度及肿瘤直径有关(P均<0.05);cyc lin E的表达与病理分化程度及淋巴结转移有关(P<0.01,P<0.05).Kap lan-M e ier单因素分析宫颈癌患者生存时间与p57k ip2,cyc lin E阳性表达有关(P<0.01,P<0.01).结论:p57k ip2的低表达或缺失和/或cyc lin E的高表达可能在宫颈癌发生发展中起重要作用,与不良预后有关,两者在宫颈癌细胞周期调控中可能存在着负反馈调节机制.展开更多
Objective： To observe the effects of p38 mitogen activated protein kinase （MAPK） inhibitor SB203580 by intrathecal injection on the pain behavior and the spinal proinflammatory cytokines in a rat model of bone canc...Objective： To observe the effects of p38 mitogen activated protein kinase （MAPK） inhibitor SB203580 by intrathecal injection on the pain behavior and the spinal proinflammatory cytokines in a rat model of bone cancer pain induced by breast cancer cells. Methods： Eleven rats were used to establish the models of bone cancer pain, six rats were treated by intrathecal SB203580 injection, and the other 5 were as the controls. The paw withdrawal latency （PWL）, histology and the spinal levels of IL-1β and TNF-α were detected. Results： All the 11 rats presented evident bone destruction and thermal hyperalgesia after intra-tibial injection of breast cancer cells. No effect of SB203580 on the bone destruction was observed. However, following intrathecal injection of SB203580, the left PWLs （12.12± 1.26 s at 16 days and 12.99 ± 1.65 s at 19 days） were significant higher than that of controls （9.05 ± 1.08 s at 16 days and 8.55 ± 1.60 s at 19 days）, P 〈 0.05. Meanwhile, inkathecal injection of SB203580 evidently reduced the levels of spinal IL-1β and TNF-α. Conclusion： Intrathecal injection of SB203580 in a rat model of bone cancer pain cannot prevent the tibial destruction but significantly depress the thermalgia sensitivity, which might result from inhibiting inkacellular p38 MAPK signaling transduction, and thereby reducing the release of the proinflammatory cytokines.展开更多
文摘目的:探讨抑癌基因p57k ip2和细胞周期素E(cyc linE)在宫颈癌发生发展中的作用及其与临床病理及预后的意义.方法:采用免疫组化二步法检测p57k ip2和cyc lin E在48例宫颈癌、13例宫颈上皮内瘤变(C IN)和15例正常宫颈组织中的表达.结果:p57k ip2在宫颈癌中的阳性表达率为21%,低于正常组(93%)和C IN组(77%),差异有显著性意义(P<0.01).cyc lin E在宫颈癌中的阳性表达率为77%,明显高于正常组(13%),差异有显著性意义(P<0.01).在宫颈癌组织中p57k ip2和cyc lin E的表达呈负相关(r=-0.597,P<0.01).p57k ip2的表达与病理分化程度及肿瘤直径有关(P均<0.05);cyc lin E的表达与病理分化程度及淋巴结转移有关(P<0.01,P<0.05).Kap lan-M e ier单因素分析宫颈癌患者生存时间与p57k ip2,cyc lin E阳性表达有关(P<0.01,P<0.01).结论:p57k ip2的低表达或缺失和/或cyc lin E的高表达可能在宫颈癌发生发展中起重要作用,与不良预后有关,两者在宫颈癌细胞周期调控中可能存在着负反馈调节机制.
基金a grant from the National Nature Sciences Foundation of China (No. 30672426).
文摘Objective： To observe the effects of p38 mitogen activated protein kinase （MAPK） inhibitor SB203580 by intrathecal injection on the pain behavior and the spinal proinflammatory cytokines in a rat model of bone cancer pain induced by breast cancer cells. Methods： Eleven rats were used to establish the models of bone cancer pain, six rats were treated by intrathecal SB203580 injection, and the other 5 were as the controls. The paw withdrawal latency （PWL）, histology and the spinal levels of IL-1β and TNF-α were detected. Results： All the 11 rats presented evident bone destruction and thermal hyperalgesia after intra-tibial injection of breast cancer cells. No effect of SB203580 on the bone destruction was observed. However, following intrathecal injection of SB203580, the left PWLs （12.12± 1.26 s at 16 days and 12.99 ± 1.65 s at 19 days） were significant higher than that of controls （9.05 ± 1.08 s at 16 days and 8.55 ± 1.60 s at 19 days）, P 〈 0.05. Meanwhile, inkathecal injection of SB203580 evidently reduced the levels of spinal IL-1β and TNF-α. Conclusion： Intrathecal injection of SB203580 in a rat model of bone cancer pain cannot prevent the tibial destruction but significantly depress the thermalgia sensitivity, which might result from inhibiting inkacellular p38 MAPK signaling transduction, and thereby reducing the release of the proinflammatory cytokines.
