In present paper, one of the T-DNA insertional embryonic lethal mutant of Arabidopsis is identifled and designated as acd mutant. The embryo development of this mutant is arrested in globular stage. The cell division ...In present paper, one of the T-DNA insertional embryonic lethal mutant of Arabidopsis is identifled and designated as acd mutant. The embryo development of this mutant is arrested in globular stage. The cell division pattern is abnormal during early embryogenesis and results in disturbed cellular differentiation. Most of mutant embryos are finally degenerated and aborted in globular stage. However, a few of them still can germinate in agar plate and produce seedlings with shorter hypocotyl and distorted shoot meristem. To understand the molecular basis of the phenotype of this mutant, the joint fragment of T-DNA/plant DNA is isolated by plasmid rescue and Dig-labeled as probe for cDNA library screening. According to the sequence analysis and similarity searching, a 936bp cDNA sequence (EMBL accession#: Y12555) from selected positive clone shows a 99.8 % (923/925bp) sequence homology with Alanyl-tRNA Synthetase (A1aRS) gene of Arabidopsis thaliana. Furthermore, the data of in sitll hybridization experiment indicate that the expression of AlaRS gene is weak in early embryogenesis and declines along with globular embryo ’development’ in this mutant.Accordingly, the reduced expression of AlaRS gene maybe closely related to the morphological changes in early embryogenesis of this lethal mutant.展开更多
We prove that there are only finitely many diffeomorphism types of curvature-adapted equifocal hypersurfaces in a simply connected compact symmetric space.Moreover,if the symmetric space is of rank one,the result can ...We prove that there are only finitely many diffeomorphism types of curvature-adapted equifocal hypersurfaces in a simply connected compact symmetric space.Moreover,if the symmetric space is of rank one,the result can be strengthened by dropping the condition curvature-adapted.展开更多
This research was carried out in the area of experimental embryology, which covers from animal fertilization to cloning. Two achievements in the field of reproductive bilogy have created major influence in the past 50...This research was carried out in the area of experimental embryology, which covers from animal fertilization to cloning. Two achievements in the field of reproductive bilogy have created major influence in the past 50 years, One is the discovery of sperm capacitation and the establishment of in vitro fertilization technology, and the other is the birth of somatic cell nuclear transfer animals. Although researches in reproductive bilogy have developed very quickly and a lot of literatures have been published. many basic theoretical questions are still remained illusive. This paper gives a brief description of our researches during the past decade.展开更多
文摘In present paper, one of the T-DNA insertional embryonic lethal mutant of Arabidopsis is identifled and designated as acd mutant. The embryo development of this mutant is arrested in globular stage. The cell division pattern is abnormal during early embryogenesis and results in disturbed cellular differentiation. Most of mutant embryos are finally degenerated and aborted in globular stage. However, a few of them still can germinate in agar plate and produce seedlings with shorter hypocotyl and distorted shoot meristem. To understand the molecular basis of the phenotype of this mutant, the joint fragment of T-DNA/plant DNA is isolated by plasmid rescue and Dig-labeled as probe for cDNA library screening. According to the sequence analysis and similarity searching, a 936bp cDNA sequence (EMBL accession#: Y12555) from selected positive clone shows a 99.8 % (923/925bp) sequence homology with Alanyl-tRNA Synthetase (A1aRS) gene of Arabidopsis thaliana. Furthermore, the data of in sitll hybridization experiment indicate that the expression of AlaRS gene is weak in early embryogenesis and declines along with globular embryo ’development’ in this mutant.Accordingly, the reduced expression of AlaRS gene maybe closely related to the morphological changes in early embryogenesis of this lethal mutant.
基金supported by National Natural Science Foundation of China(Grant Nos.11071018 and 11001016)the Specialized Research Fund for Doctoral Program of Higher Education(Grant No.20100003120003)the Program for Changjiang Scholars and Innovative Research Team in University
文摘We prove that there are only finitely many diffeomorphism types of curvature-adapted equifocal hypersurfaces in a simply connected compact symmetric space.Moreover,if the symmetric space is of rank one,the result can be strengthened by dropping the condition curvature-adapted.
文摘This research was carried out in the area of experimental embryology, which covers from animal fertilization to cloning. Two achievements in the field of reproductive bilogy have created major influence in the past 50 years, One is the discovery of sperm capacitation and the establishment of in vitro fertilization technology, and the other is the birth of somatic cell nuclear transfer animals. Although researches in reproductive bilogy have developed very quickly and a lot of literatures have been published. many basic theoretical questions are still remained illusive. This paper gives a brief description of our researches during the past decade.
