不同输精方式和有效精子数量对吉林马情期受胎率的影响

为研究有效精子数量对吉林马母马情期受胎率的影响,于2018—2020年选取健康且发情周期正常的300匹吉林马母马进行试验,采用子宫体常规输精和子宫角深部输精方式及不同有效精子数量对吉林马母马进行人工授精。结果表明,子宫体常规输精一次有效精子数达2×10^(8)个以上或子宫角深部输精一次有效精子数达0.5×10^(8)个以上,吉林马母马情期受胎率均可达60.00%以上;子宫角深部输精方式吉林马母马平均情期受胎率为69.80%,子宫体常规输精方式吉林马母马平均情期受胎率为45.78%,二者差异极显著(P<0.01)。这说明采用子宫角深部输精能显著提高吉林马母马情期受胎率,显著降低一次输精有效精子数量,提高种公马精液利用效率。

陇东绒山羊细管鲜精有效精子数量对受胎率影响的研究

为了研究陇东绒山羊0.25mL细管精液所含有效精子数量对受胎率的影响。对种公羊采精后,经过精液品质检查,根据精子活力和密度,计算出有效精子数量,在0.25m L细管中,分为5组分别含有6×106、10×106、15×106、20×106和30×106不同有效精子数量。人工授精后,试验1组到试验5组受胎率分别为58.93%、81.03%、83.33%、86.67%和87.09%,试验1组与其他四组差异极显著(P<0.01),但试验组2、试验组3、试验组4和试验组5,四组之间差异不显著(P>0.05)。通过试验研究,0.25mL细管精液中含有10×106个有效精子以上,人工授精2次,受体绒山羊受胎率即可达到81.03%以上的,为最大限度利用陇东绒山羊鲜精提供科学依据。

Uncertainty Evaluation of Boar Fresh Semen Quality

According to JJF1059.2-2012 and CNAS-GL06, the measurement uncertainty of four indicators （semen volume, sperm motility and validity, progressive motility, abnormal sperm percentage） of boar fresh semen, as well as its influencing factors, was analyzed. The results showed that the measurement uncertainty of semen volume and progressive motility caused by inspectors was low, and their measurement results reliability was high. There were small differences in measurement results of semen volume and progressive motility among inspectors. However, the measurement uncertainty of sperm motility and abnormal sperm percentage was relatively large, and great differences were found among different inspectors. Therefore, the training of inspectors＇ measuring sperm motility and abnormal sperm percentage should be strengthened, and the comparison of measurement results among inspectors should be performed in the future.