细胞外基质分子在人胚中肾有核血细胞的表达

目的：观察细胞外基质分子在第4～8周人胚中肾有核血细胞的发育和迁移过程中的表达情况。方法：第4～8周的人胚行H—E和免疫组织化学染色，光镜观察。结果：发育第4、5周中肾的有核血细胞、原始肾小球、肾小管以及散在分布的间充质细胞均强表达纤连蛋白、层连蛋白、胶原蛋白Ⅰ、细胞间黏附分子。第7周，中肾有核血细胞中仅有部分表达上述分子。到第8周，仅在个别有核血细胞和肾小管上皮细胞表达。到第7周，中肾内有核血细胞几乎都表达整合素β1。肾内其他结构无明显的整合素β1表达。结论：在人胚发育第4～8周，中肾的有核血细胞表达多种细胞外基质分子。这些分子可能以自分泌方式参与肾内有核血细朐在中肾的发育。

人胚中肾发育和生长因子及其受体的表达

目的：观察第3～8周人胚中肾的发育和生长因子及其受体的表达。方法：第3～8周人胚，H—E和免疫组织化学染色，光镜观察。结果：人胚中肾于第3周末出现，并从头-尾方向同时进行生长和退化过程。中肾于第8周退化。发育第5周中肾的肾小管和肾小球细胞强表达碱性成纤维细胞生长因子（bFGF）和转化生长因子β1（TGF-β1）及其受体。中肾内有核血细胞于第4周出现，第7周数量达到高峰。表皮生长因子、胰岛素样生长因子Ⅰ、bFGF和TGF-β1及其受体在发育第5～8周中肾的有核血细胞表达。结论：人胚中肾的发育主要在第3～7周。第6～7周人胚中肾内的有核血细胞数量达高峰。人胚中肾内的有核血细胞、肾小管内皮细胞、肾小囊和肾小球细胞表达多种生长因子。

微小RNA在心肌重构及心力衰竭中的作用

白1993年Wightman等[1]首次发现第一个微小RNA（miRNA）至2011年,在miRNA数据库18.0版本中登记的miRNA总数已经达21 643个,其中人类基因组1 424个,调控约60％的编码基因.每个miRNA调控多个不同的靶mRNA,已证实其参与调控细胞发育、分化、增殖、代谢、凋亡、肿瘤生成、造血等多种生物过程.2008年研究人员发现miRNA可在血浆、血小板、红细胞及有核血细胞中检测到,并指出血浆中miRNA高度稳定,每一种疾病都有特定的miRNA表达谱.

Effect of Zishenshengxue capsule on myelosuppression in mice induced by cyclophosphamide

OBJECTIVE:To explore the inhibitory effect of Zishenshengxue capsule(ZSC) on myelosuppression in mice induced by cyclophosphamide.METHODS:Kunming mice were randomly assigned into a control group,myelosuppression group,or groups for mice with myelosuppression receiving high dose ZSC,middle dose ZSC,low dose ZSC or Yixuesheng.Myelosuppression was induced by peritoneal injection of cyclophosphamide.ZSC and cyclophosphamide were administered simultaneously.The numbers of peripheral blood cells and bone marrow karyocytes were counted.Cell proliferation activity and colony formation of granulocyte-monocyte series hemopoietic progenitor cells(C-GMs) and cell cycle were detected.RESULTS:The numbers of white blood cells in the middle and high dose ZSC groups were significantly increased at the 12th and 13th day(P<0.01) and bone marrow karyocytes and cell proliferation activity increased in the high dose ZSC group(P<0.01) compared with the myelosuppression group.C-GMs of middle and high dose ZSC groups significantly increased(P<0.01).The percentage of G 1 phase in the high dose ZSC group decreased(P< 0.01) and the percentage of S and G 2 phase increased(P<0.01).CONCLUSION:ZSC increased the numbers of peripheral white blood cells,bone marrow karyocytes and C-GMs.ZSC also increased cell proliferation activity and removed the G 1 phase block.Thus,ZSC could reduce myelosuppression in mice induced by cyclophosphamide.