应当令禁砍伐蚬木加工砧板

蚬木是国家定为二级保护的珍贵树木。然而近年来南宁地区的龙州、大新、宁明县和凭祥市,蚬木遭到非法砍伐的情况日趋严重,一些不法分子乘中越边境贸易点开放,越南边民带蚬木砧板过境交易之机,肆意盗伐自然保护区内外的蚬木。

Evaluation of Productivity and Light Quality in Two High Density Dwarf Rootstock Apple Orchards in Central China

[Ohjective] The aim of the study is to investigate the productivity and light quality in two high density M26 dwarf rootstock apple orchards in central China so as to provide some management guidance for close planting of dwarf rootstock apples.[Method] The technical parameters of individual trees and group parameters as shoot number and composition and canopy coverage were determined, and the light quality in the canopy, fruit production and quality were investigated. [Result] Slender spindle （SS） orchard has 54 thousands shoots per 667 m^2. Coverage rate is 76%. Leaf area index is 1.9. The ratio of long, medium and spur shoots is 1：1：8. Fruit yield is 3 263 kg/667 m^2 with 85% first grade fruit. Light interception in the canopy is 58% while the ratio of canopy with good light is 65%. Modified slender spindle （MSS） orchard has 93 thousands shoots per 667 m^2 and the coverage is 77%. Leaf area index is 3.3. The ratio cf long, medium and spur shoots is 1：2：7. Fruit yield is 3 931 kg/667 m^2 with 85% first grade fruit. The light interception in the canopy is 73% while the ratio of canopy with good light is 35%. [Conclusion] Apple orchard with M26 dwarf rootstock trained as SS and MSS tree form in medium planting density may be useful to the management of the similar orchards in Central China.

Study on Grafting and Cultivation of ‘Spartan'Blueberry

The results of grafting improvement and cultivation trials in the Yangtze River Delta of China showed that the rootstock cultivar had a great influence on plant growth and development, fruit yield and quality of ＂Spartan＂ blueberry. Among the seven rootstock-scion combinations, the northern highbush blueberry （Vaccinium corymbosum） cultivar ＂Croaton＇-＇Spartan＇ combination showed well-healed graft unions, large branch amount, early fruit bearing, high yield, excellent fruit quality and almost no tillers. As a kind of rootstock, ＂Croaton＇ was better than non-grafted ＇Spartan＇ and the two rabbiteye blueberry cultivars ＇Premier＇ and ＇Gardenblue＇. The grafting pattern showed no significant stimulating effect on the plant growth and development, fruit yield and quality of ＇Spartan＇ blueberry in this area, but the overall performance of greenwood cleft grafting was better than that of inlaid bud grafting. Suitable rootstock cultivar and grafting pattern significantly improve plant arowth and development, fruit vield and aualitv of ＇Spartan＇ blueberry.

赫哲制革业述略

赫哲族是我国人口最少的民族之一,主要分布在黑龙江、松花江、乌苏里江流域。赫哲人沿江河而居,渔猎生产是其最主要的经济生活来源。在渔猎生产中,赫哲人也创造和发展了自己的民族制革业。赫哲族制革业能够产生并有较大的发展,是与人们的生活需要和赫哲妇女的辛勤劳动分不开的。

年夜饭

一年中最重要的一顿饭就是年夜饭。刚吃过午饭,择菜的、宰鱼的、剁鸡的、和面的,就各司其职、有条不紊地忙开了,不锈钢盆、陶瓷碗、竹筷子、木砧3(zhēn)板,叮叮当当,此起彼伏。桌子上,碗碟杯盘挤作一团,菜盘里,珍馐佳肴热气腾腾,年的味道如约而至。

顾客第一 细节体现

客人永远是厨师水平的最好评判,一个好的厨师,不仅是把烹调当成一种职业,更要设身处地站在顾客的角度考虑问题。

Effects of Drought Stress on Antioxidant System of Leaves from Different Citrus Rootstocks

[Objective] The paper aims to study the effects of drought stress on antioxidation system of different Citrus rootstock leaves. [Method] Potted plant experiments were carried out to control the water content of soil in pots,in order to investigate the effects of drought stress on antioxidation system activity （SOD,POD and CAT） and the content of protein,GSH,MDA and O-·2 from the leaves of Shandong Zhike,Ningminju,Yangshuojinbaosuanju,Huapijinju and Guizhi No.1. [Result] The content of chlorophyll a,chlorophyll b and chlorophyll （a＋b） from the test 5 breeds decreased with the enhancement of drought stress,and there were significant differences between them and the control under the severe stress; the activities of SOD,POD and CAT from the 5 breeds increased as the drought stress strengthened; the content of soluble protein declined while that of GSH,MDA and O-·2 rose because of the drought stress. [Conclusion] This research provided a good reference to choose the breeds of citrus rootstocks in arid and semi-arid areas.

Regeneration of Leaves in vitro in Cherry Rootstock Colt

Plants were regenerated from leaves of cherry rootstock Colt by two methods,the frequencies were 48 3% and 21 3%.Leaves were cultured on MS medium supplemented with NAA 1 0mg/L,KT 3 0mg/L and ZT 0 25mg/L.After 5～7 days leaves dedifferentiated,and formed callus.About 25 days later,callus grew into greenish or pink compact ones,the induction frequency was 100%.The color and structural feature of callus depended on the medium,culture condition and physiology phase of leaves.To induce callus,NAA was the main factor.Leaves rooted in medium only with NAA.In order to inhibit rooting and make callus grow fast,KT and ZT were added to the medium.Leaves cultured without light could quickly form callus during the initial stage of dedifferentiation.Young leaves dedifferentiated more easily than old leaves.After had been cultured about 50 days,callus were transferred to MS medium supplemented with NAA 0 2mg/L,IAA 0 5mg/L,6 BA 0 5mg/L,KT 1 0 mg/L and GA 0 5mg/L,and redifferentiated at a frequency of 21 3%.The callus of Colt leaves were difficult to redifferentiate.GA was important in the redifferentiation of callus.The result showed that callus could redifferentiate in all the mediums with GA,and could not redifferentiate without GA.The ability of redifferentiation of big callus was higher than that of small ones.And the physiology character of callus was also important.High regeneration frequency was obtained from greenish or pink compact callus.The frequency of regeneration of petioles was higher than that of leaves.Leaf petioles' regeneration need high concentration of cytokinin(more than 5 0mg/L).Leaf petioles were cultured on MS medium supplement with 6 BA 6 0mg/L,NAA 1 0mg/L and GA 0 5mg/L,and regenerated at a frequency of 48 3%.

Tolerance to Iron-Deficiency Stress of Three Apple Rootstock Species in Hydroponic System

[Objective] Iron deficiency is one of the most important crop element deficiencies in the Loess Plateau of northwestern China. The selection for crop cultivars that are tolerant to low iron levels could be one of the approaches to solving the problem and improving crop production. [Method] Three major apple root stock species （Malus prunifolia, Malus sieversii and Malus baccata） were selected to evaluate their tolerance to iron defciency in hydroponic system. A 3×2 factorial pot experiment was conducted with three replicates in a greenhouse at Gansu Agricultural University, Lanzhou, China. [Result] The SOD, POD and CAT activities in roots and stems of the 3 root stock species in Fe-defcient Hoagland solution decreased, however Malus sieversii got the less reduction and had better root architecture and growth than the other species. The aboveground biomass, plant height, chlorophyll content, total root length and lateral root number were correlated positively with iron-defciency stress tolerance. The species’ tolerance to iron-defciency from high to low was M. sieversii’s〉M. baccata’s〉M. prunifolia’s. Moreover, the improvement of some morphological features such as root length, above-ground biomass, plant height and lateral root number in apple could be conducive to breeding cultivars with tolerance to iron-defciency stress. [Conclusion] Malus sieversii had better tolerance to iron-defciency stress than the others in this study.

Establishment of a Rapid Propagation Protocol of GLRaV-3,GFKV,and GRSPa Free Grapevine Rootstocks

Nursery plant propagation by grafting has been widely used in modern viticulture to minimize the damage caused by biotic and abiotic stresses.In grapevine(Vitis spp.),an effective way to control disease damage is to provide producers and growers with pathogen-free stock plants.In this study,five grapevine rootstock varieties,‘SO4’,‘101-14’,‘5BB’,‘110R’and‘1103P’,were selected as explants to establish an in vitro culture protocol,and three species of grapevine viruses were tested by real-time RT-PCR.The results showed that MS medium supplemented with 0.2 mg/L IBA,1.0 mg/L 6-BA,0.5 mg/L KT,4.0 mg/L adenine for culture initiation,and WPM supplemented with 0.2 mg/L IBA for subculture were suitable for all five rootstock varieties,with multiplication coefficients ranging from 1.6 to 4.4.Virus testing showed that single RT-PCR was more effective for detecting the three viruses compared to double or triple RT-PCR.Only plantlets free from the aforementioned viruses were retained for subculture.Plantlets were hardened at room temperature under natural lighting in Hoagland solution for 2 weeks and transplanted to pots filled with mixed media in a greenhouse.This protocol is applicable for rapid propagation of the five grapevine rootstock varieties and can be used for commercial production of virus-free grapevine stocks.

Effect of Rootstocks on Growth, Yield and Bean Quality of Coffea canephora Clones

Coffee cultivation on dry area lately faces more often drought condition because of global warming effect. One effort to solve the problem is by using tolerant clones or varieties as the rootstocks. The aim of this research is to observe influence of BP 308, BP 409, and Exelsa as rootstocks, on growth, yield and bean quality of BP 409, BP 534, BP 936 and BP 939 clones as scions. The research was conducted in Temanggung district of Central Java, Indonesia, using randomized complete block design with 5 replications 10 plants per replication. The result showed that rootstock influenced stem height and number of branches, leaf relative water content （RWC） during dry season, but not stem diameter. BP 308 and BP 409 rootstocks supplied water more than Exelsa, it seem RWC scion on both rootstocks were higher （82.0%） than that of Exelsa （80.0%）. Exelsa rootstock cause scion grow more slowly, so bean yield was lower than on BP 308 and BP 409. Yield accumulation until 4 years old on Exelsa was 55% to BP 308, and 52.2% to BP 409 rootstock. Yield of BP 939/BP 308 and BP 409/BP 409 （scion/rootstock） tend to the highest. Rootstocks did not influence percentage of normal and abnormal beans and the outturns. Bean outturn was more influenced by clones. Exelsa rootstock improves caffeine content, body, astringent and bitterness characters of scion cherries, but decreases fragrance and aroma characters of scion yield. It is concluded that farther taxonomical relationship of rootstock, their influence on scion growth, yield and cup test characters are stronger.

Influence of Quince Rootstocks on Entomosporium Leaf Spot （Entomosporium mespili） Susceptibility in European Pear cv. Abate Fetel

Entomosporium leaf spot （ELS） is caused by the fungus Fabraea maculata （anamorph： Entomosporium mespili） and affects most pear cultivars and quince rootstocks in Brazil. The aim of this study was to characterize the effect of Adams, EMA and EMC quince rootstocks on ELS in European pear cultivar ＂Abate Fetel＂ in Southern Brazil, during the 2009/2010, 2010/2011 and 2011/2012 growing season. The incidence and severity of disease was quantified weekly in 100 randomly leaves distributed in four medium-height branches per plant with eight replications. Disease progress curves of ELS were constructed and the epidemics compared according to： （1） the beginning of symptoms appearance （BSA）; （2） the time to reach the maximum disease incidence and severity （TRMDI and TRMDS）; （3） area under the incidence and severity disease progress curve （AUIDPC and AUSDPC）. The data were analyzed by linear regression and adjusted for three empirical models： Logistic, Monomolecular and Gompertz. The Abate Fetel cultivar under all rootstocks evaluated was susceptible to E. mespili. However, there were significant differences in ELS intensity among rootstocks evaluated. The highest ELS intensities were observed in combinations with EMA and Adams quince rootstock. Abate Fetel cultivar grafted on EMC quince rootstock showed all epidemiological variables results significantly different when compared with EMA quince rootstock. EMC quince rootstock induced late resistance compared with the other considerated rootstocks. The Logistic model was the most appropriates to describe the ELS progress of Abate Fetel cultivar under all rootstocks evaluated in the edafoclimatic conditions of Southern Brazil, during the 2009/2010, 2010/2011 and 2011/2012 growing season.

Growth, Yield and Fruit Quality of Tahiti Lime on Eight Standard Rootstocks Affected by Soil Depth

Two Tahiti lime （Citrus latifolia Tan.） experiments were carried out to evaluate the performance of eight standard rootstocks on two different soil locations at Tecoman Colima, Mexico. Rootstocks were selected by their tolerance to Citrus tristeza virus （CTV） and acceptable adaptation to semi-add hot tropics environment. The first trial was planted in ＂Cerrito de Aguilar＂ （CA） location which had sandy-loam soil with a fluctuant water table at 100 cm depth. The second experiment was planted in ＂Crucero Tecoman＂ （CT） on a sandy deep soil with no water table restriction. At CT location the best Tahiti lime growth, yield and fruit quality were observed on Alemow rootstock. The same combination at CA showed a poor performance and was affected by diseases due to high moisture conditions by the superficial water table. Taiwanica, Swingle citrumelo and Rangpur lime rootstocks were less productive than Alemow, but showed a good adaptation and acceptable yield on both soil conditions. Among them, Swingle citrumelo was a promising rootstock because it showed standard tree growth, good yield and excellent fruit quality. Swingle citrumelo also showed an acceptable performance on soil with high presence ofPhytophthora sp. and moderate levels of calcium carbonate （near to 3,500 ppm）. Taiwanica and Rangpur lime performed well during the six years of the experiment, but trees began to be affected by root diseases. Better performance of most Tahiti lime/rootstocks were found on sandy depth soil than on superficial wet soils.

Physical mapping of three fruit ripening genes: Endopolygalacturonase, ACC oxidase and ACC synthasefrom apple (Malus x domestica) in an apple rootstock A106 (Malus sieboldii)

The apple rootstock, A106 (Malus sieboldii), had 17 bivalents in pollen mother cells at meiotic metaphase 1,and 17 chromosomes in a haploid pollen cell. Karyotypes were prepared from root-tip cells with 2n= 34 chromosomes. Seven out of 82 karyotypes (8.5%) showed one pair of satellites at the end of the short arm of chromosome 3. C-bands were shown on 6 pairs of chromosomes 2, 4,6, 8, 14, and 16 near the telomeric regions of short arms.Probes for three ripening-related genes from Malus x domestica: endopolygalacturonase (EPG,0. 6 kb ) , ACC oxidase (1.2 kb), and ACC synthase (2 kb) were hybridized in situ to metaphase chromosomes of A106. Hybridization sites for the EPG gene were observed on the long arm of chromosome 14 in 15 out of 16 replicate spreads and proximal to the centromere of chromosomes 6 and 11. For the ACC oxidase gene, hybridization sites were observed in the telomeric region of the short arm of chromosomes 5 and 11 in 87% and 81% of 16 spreads respectively proximal to the centromere of chromosome 1 in 81% of the spreads, and on the long arm of chromosome 13 in 50% of the spreads. Twenty five spreads were studied for the ACC synthase gene and hybridization sites were observed in the telomeric region of the short arm of chromosome 12 in 96% of the spreads, chromosomes 9 and 10 in 76% of the spreads, and chromosome 17 in 56% of the spreads.

Research of Agronomic Characteristics of Peach Rootstocks on Calcareous SoilmTwelve Yearly Results

The experimental orchard on calcareous soils in the Tikves region has been established in 1999, in order to investigate the influence of the rootstocks on the behaviour of the peach trees. In the experiment we evaluated next rootstocks： Nemaguard （Prunus persica × Prunus davidiana）, seedlings of GF 677 （Prunus persica ×Prunus amygdalus）, hybrids BMVA 1, BMVA 2, VAF 215 and Autochthones type and GF 677 （Prunus persica × Prunus amygdalus） clonal. As control rootstocks in our experiment was used seedlings from vineyard peach （Prunus persica）. The experiment was established with one year old trees from Redhaven variety. Agronomical characteristics （vegetative growth, occurrence of leaf chlorosis, tree mortality, yield, productivity and fruit quality） of the trees on different rootstocks have been measured over a twelve-year period （1999-2010）. Significant differences on the level of P 〈 0.05 have been observed on vigour of the trees. The most vigorous trees were those on GF 677 clonal, GF 677 seedling and BMVA 2. The trees on Vineyard peach and Nemaguard was the weakest ones. The highest cumulative yield was recorded on GF-677 clonal rootstock, and the lowest on Nemaguard. Occurrence of chlorosis was the highest at Vineyard peach, Nemaguard and Autochthones hybrid, GF 677 clonal was the only rootstock without iron chlorosis. The percent of tree mortality to twelfth year was the highest at Autochthones hybrid （33.3）, and at GF 677 clonal and BMVA 1 had not tree mortality.

Vegetative Habitus and Fruit Production of Self-rooted Cherry Cultivar ＇Hedelfingen＇ Wild Type and Somaclonal Grafted on ＇Gisela 6＇ and ＇Colt＇ Rootstock

The study considers the morphological and physiological behaviour of self-rooted sweet cherry CV （Cultivar） ＇Hedelfinger＇ wild type （H） and somaclonal （HS） grafted on ＇Gisela 6＇ and ＇Colt＇ rootstock. The somaclonal showed reduced vegetative vigour without any variation of the natural tree＇s architecture. The rootstock ＇Gisela 6＇ caused change in genotype habitus inducing a spreading shape, while ＇Colt＇ increased trunk diameter and height. Fruit quality and size were not affected by genotype nor rootstock. ＇Gisela 6＇, from these preliminary data, had proved the most suitable rootstock for both genotypes since it reduced the tree size and vigor and induced early bearing and the production of a greater number of fruiting spurs.

Shoot and Root Growth of Jatropha curcas Accessions Prospective for Rootstock on Rocky and Heavy Soil

To find Jatropha curcas L. accessions with characteristics suitable for rootstock plants, 11 selected accessions were analyzed in response to heavy soil. Six-week-old of jatropha were grown in 11 L ofentisols （M1） and grumusols （M2） soil for 10 weeks, while andosols soil richer in organic carbon were used as control media （M0）. Growth of shoot and primary as well as secondary roots were observed during and at the end of the experiment. Macro and micro nutrient uptake was also analyzed at the end of experiment. Ml and M2 caused dramatically reduction of roots as well as shoot growth of all accessions. The reduction of growth component was in accordance to all nutrient uptakes except Ca and Mg. The data showed that type of soil was one of important factors influencing growth and development of J. curcas. There were 4 accessions i.e. Sumatra-l （S1）, Sumatra-2 （S2）, Java-3 （J3） and Java-2 （J2） which had better roots and shoot characteristics that were potential to be used as rootstock plants.

Effects of Trifoliate Interstocks on Growth and Yield of Mexican Lime Trees

This study was carried out to determine the effect of 21 Citrus interstocks and the control Citrus macrophylla rootstock budded with Mexican lime （Citrus aurantifolia （Chistm.） Swingle） on growth and yield. Three types of mandarin and 13 trifoliate types including ＂C-35＂ and ＂Benton＂ used as interstocks, developed vigorous trees similar or more productive than the control. ＂Morton＂ and ＂Benecke＂ citranges, ＂Pomeroy＂ trifoliate trifoliate orange and the ＂Severinia buxifolia＂ interstocks showed a moderated effect on tree growth. Flying dragon （FDT） and Hiryu trifoliate oranges performed as semi-dwarfing interstocks. These interstocks reduced the tree height, canopy volume and surface area covered by the foliage on 25%-35%, 20%-30% and 20%-30%, respectively, compared to the control. While most interstocks surpassed the assigned area of growth （24 m2） in the 7th year, ＂FDT＂ and ＂Hiryu＂ did that at the 10th year. These semi-dwarfing interstocks produced up to 80 kg/tree per year. Most treatments recorded the highest production from the 8th to 10th year, except FDT and Hiryu, which showed the highest yield （130 kg/tree） from the 5th to 7th year. This study provides useful results to face the huanglongbing （HLB） problem using moderate or semi-dwarfing interstocks for Mexican lime orchards in high density plantations.

Studies on Mode of Expression of Apple （Malus x domestica Borkh.） Bud Dormancy under Tropical and Temperate Climatic Conditions

A study was conducted to describe the progression of bud dormancy in 1-year-old apple （Malus x domestica Borkh） shoots grown at two contrasting climatic conditions （Belgium, temperate and Ethiopia, tropics）. The experiment was carried out on ＂Golden＂ and ＂Gala＂ cultivars for two consecutive years （2010/2011 and 2011/2012）. Moreover, a validation experiment was conducted on ＂MM106＂ apple rootstock during 2010/2011 only in Ethiopia. Variations in inverse of time to 50% budburst were interpreted in terms of evolution of growth capacity of the buds. Despite differences observed with chilling accumulation later in winter or early in spring, depending on environments, depth of endodormancy intensity during winter can be summarized as follows： buds from pruned shoots were less endodormant than terminal buds of the intact shoots and terminal buds were more endodormant than the dormancy intensity of upper buds of the disbudded shoots, suggesting proximal buds can grow more readily than does terminal ones. Our results provide evidence for the existence of a considerably strong paradormancy inhibition by distal shoot parts and buds, which was more pronounced in Ethiopia than in Belgium, highlighting the importance of designing and applying appropriate pruning and dormancy avoidance strategies in mild-winter climates. Finally, as still there is knowledge gap on bud dormancy progression and its control mechanism especially under mild climates, our study highlights the need for further in-depth research using biological and biochemical tests.

Dynamics of Growth of the Introduced Nectarine Cultivars

A research on the topic was conducted at the Experimental Station of Agriculture--Khan Krum during the period 2009-2011. The dynamics of growth and thickening of the cultivars Independence, Nectagrand 2, Aureliogrand, Fantasy, Kassiopeia and Caldessi 2000 grafted on seedling rootstock （Elbert） and clonal GF-677 have been studied in the second year of nursery. The aim of the study was to investigate the growth characteristics of the introduced cultivars of nectarines grafted on traditional seedling rootstock （Elbert） and GF-677. At the beginning of the vegetation period of the seed cultivar rootstock （Elbert） has strong growth and thickening of the stem. The dynamics of growth and thickening of the stem depend on the used rootstock and cultivar. The obtained planting material corresponds to the existing quality standards.