To convert the non biodegradable sodium lignin sulfonate into biodegradable substances, the sodium lignin sulfonate in the water was ozonized and the pH value, dissolved organic carbon(DOC), ultraviolet absorbency at...To convert the non biodegradable sodium lignin sulfonate into biodegradable substances, the sodium lignin sulfonate in the water was ozonized and the pH value, dissolved organic carbon(DOC), ultraviolet absorbency at λ =254 nm(UVA) and the biodegradability of the ozonation effluent were measured. The non biodegradable sodium lignin sulfonate can be partly converted into biodegradable substances by ozonation (about 38 76%). In the ozonation process, there is little DOC decrease, but much UVA decrease and obvious pH drop.展开更多
Pretreatment of the corn stover powder by dilute sulphuric acid （solid-liquid ratio 1 ： 20） at 130 for 30 min was carried out with 89.09% of the hemicellulose removed. After filtration, the xylose-rich corn stover ...Pretreatment of the corn stover powder by dilute sulphuric acid （solid-liquid ratio 1 ： 20） at 130 for 30 min was carried out with 89.09% of the hemicellulose removed. After filtration, the xylose-rich corn stover pretreatment liquid, whose fermentable sugar was from hemicellulose hydrolysis only, consisting of 81.16% xylose and 15.27% glucose, was used to cultivate genetic recombinant Escherichia coli BL21 with human-like collagen （HLC） expression enhanced by 50.00% and 63.71% xylose consumption.展开更多
In this study, the rice straw was hydrolysed by using 3.0% （w/v） H2SO4 followed by enzymatic hydrolysis. The rice straw hydrolysate obtained was treated with charcoal powder and the optimal condition of detoxificati...In this study, the rice straw was hydrolysed by using 3.0% （w/v） H2SO4 followed by enzymatic hydrolysis. The rice straw hydrolysate obtained was treated with charcoal powder and the optimal condition of detoxification with charcoal powder was investigated. The results showed that the optimal condition for detoxification was the use of 2.5 grams of non-sterilized charcoal powder in 100 mL hydrolysate. The mixture was operated at pH 5.0, 30 ℃ and 160 rpm for 5 min. The detoxified hydrolysate was then used for ethanol production using P. stipitis TISTR 5806. The condition of the detoxified hydrolysate fermentation which gave maximum ethanol concentration of 21 g/L was at pH 5.0, 30 ℃ and 160 rpm for 72 h. Without detoxification, the P. stipitis TISTR 5806 could not however utilize the hydrolysate for ethanol production.展开更多
An efficient method for the simultaneous quantification of three flavone aglycones was developed and validated for the control of flavonoids in Flos Chrysanthemi （FC）. The method employed mixed solvent of hydrochlor...An efficient method for the simultaneous quantification of three flavone aglycones was developed and validated for the control of flavonoids in Flos Chrysanthemi （FC）. The method employed mixed solvent of hydrochloric acid （HC1） and methanol to extract and hydrolyze flavone glycosides simultaneously from powdered FC followed by HPLC to determine the contents of three flavone aglycones, luteolin, apigenin and diosmetin, which are the major bioactive flavones in FC. The extraction and hydrolysis conditions optimized by the orthogonal tests were as follows： powdered FC was refluxed in 80% methanol （v/v） containing 2.4 M HC1 for 2 h in 80 ℃ water bath. The HPLC separation was performed on C18 column and detected with DAD at 344 nm. The calibration curves for luteolin, apigenin and diosmetin were linear over the ranges of 2.233-71.46 μg/mL, 1.668-53.38 μg/mL and 1.410-45.11 μg/mL, their assay recoveries were 100.3%, 103.9% and 100.4%, their intra-day repeatability （R.S.D.） were 0.68%, 0.21% and 0.38% and their inter-day repeatability were 2.6%, 1.7% and 2.0%, respectively. The developed method has been successfully utilized to assay eight species of popular FC samples, especially to analyze Hangbaiju, the well-known FC in China.展开更多
文摘To convert the non biodegradable sodium lignin sulfonate into biodegradable substances, the sodium lignin sulfonate in the water was ozonized and the pH value, dissolved organic carbon(DOC), ultraviolet absorbency at λ =254 nm(UVA) and the biodegradability of the ozonation effluent were measured. The non biodegradable sodium lignin sulfonate can be partly converted into biodegradable substances by ozonation (about 38 76%). In the ozonation process, there is little DOC decrease, but much UVA decrease and obvious pH drop.
基金Supported by the Agriculture Application Investigation and Improvement Item of New Countryside Construction and Promotion Project of the Bureau of Science and Technology in Xi’an (NC08005)
文摘Pretreatment of the corn stover powder by dilute sulphuric acid （solid-liquid ratio 1 ： 20） at 130 for 30 min was carried out with 89.09% of the hemicellulose removed. After filtration, the xylose-rich corn stover pretreatment liquid, whose fermentable sugar was from hemicellulose hydrolysis only, consisting of 81.16% xylose and 15.27% glucose, was used to cultivate genetic recombinant Escherichia coli BL21 with human-like collagen （HLC） expression enhanced by 50.00% and 63.71% xylose consumption.
文摘In this study, the rice straw was hydrolysed by using 3.0% （w/v） H2SO4 followed by enzymatic hydrolysis. The rice straw hydrolysate obtained was treated with charcoal powder and the optimal condition of detoxification with charcoal powder was investigated. The results showed that the optimal condition for detoxification was the use of 2.5 grams of non-sterilized charcoal powder in 100 mL hydrolysate. The mixture was operated at pH 5.0, 30 ℃ and 160 rpm for 5 min. The detoxified hydrolysate was then used for ethanol production using P. stipitis TISTR 5806. The condition of the detoxified hydrolysate fermentation which gave maximum ethanol concentration of 21 g/L was at pH 5.0, 30 ℃ and 160 rpm for 72 h. Without detoxification, the P. stipitis TISTR 5806 could not however utilize the hydrolysate for ethanol production.
基金The Ministry of Sciences and Technology of China (Grant No. 2006BAI06A18-11)Administration and Traditional Chinese Medicine,Zhejiang,China (Grant No. 2007ZA012)
文摘An efficient method for the simultaneous quantification of three flavone aglycones was developed and validated for the control of flavonoids in Flos Chrysanthemi （FC）. The method employed mixed solvent of hydrochloric acid （HC1） and methanol to extract and hydrolyze flavone glycosides simultaneously from powdered FC followed by HPLC to determine the contents of three flavone aglycones, luteolin, apigenin and diosmetin, which are the major bioactive flavones in FC. The extraction and hydrolysis conditions optimized by the orthogonal tests were as follows： powdered FC was refluxed in 80% methanol （v/v） containing 2.4 M HC1 for 2 h in 80 ℃ water bath. The HPLC separation was performed on C18 column and detected with DAD at 344 nm. The calibration curves for luteolin, apigenin and diosmetin were linear over the ranges of 2.233-71.46 μg/mL, 1.668-53.38 μg/mL and 1.410-45.11 μg/mL, their assay recoveries were 100.3%, 103.9% and 100.4%, their intra-day repeatability （R.S.D.） were 0.68%, 0.21% and 0.38% and their inter-day repeatability were 2.6%, 1.7% and 2.0%, respectively. The developed method has been successfully utilized to assay eight species of popular FC samples, especially to analyze Hangbaiju, the well-known FC in China.
