The responses of the growth and metabolism activity of Phanerochaete chrysosporium （P. chrysosporium） to cadmium （Cd）, lead （Pb） and their combined pollution stress, were investigated in plate and liquid culture...The responses of the growth and metabolism activity of Phanerochaete chrysosporium （P. chrysosporium） to cadmium （Cd）, lead （Pb） and their combined pollution stress, were investigated in plate and liquid culture conditions. The diameter of colony, biomass ofP. chrysosporium, ligninolytic enzyme activities and bioaccumulation quantity of heavy metals were detected. The results indicated that Cd was more toxic than Pb to P. chrysosporium and the toxicity of Cd and Pb to P. chrysosporium was further strengthened under Cd＋Pb combined pollution in different culture conditions. Heavy metals Cd and Pb had indirect influence on the production of ligninolytic enzymes by directly affecting the fungal growth and metabolic activity, and by another way in liquid culture. In addition, the results provided an evidence of the accumulation of Cd and Pb on the mycelia ofP. chrysosporium.展开更多
基金Projects(21477027,51278176)supported by the National Natural Science Foundation of ChinaProject(2014A020216048)supported by the Science and Technology Planning Project of Guangdong Province,ChinaProject(2015M582363)supported by the China Postdoctoral Science Foundation
文摘The responses of the growth and metabolism activity of Phanerochaete chrysosporium （P. chrysosporium） to cadmium （Cd）, lead （Pb） and their combined pollution stress, were investigated in plate and liquid culture conditions. The diameter of colony, biomass ofP. chrysosporium, ligninolytic enzyme activities and bioaccumulation quantity of heavy metals were detected. The results indicated that Cd was more toxic than Pb to P. chrysosporium and the toxicity of Cd and Pb to P. chrysosporium was further strengthened under Cd＋Pb combined pollution in different culture conditions. Heavy metals Cd and Pb had indirect influence on the production of ligninolytic enzymes by directly affecting the fungal growth and metabolic activity, and by another way in liquid culture. In addition, the results provided an evidence of the accumulation of Cd and Pb on the mycelia ofP. chrysosporium.
