Pleurotus sp2 and Trametes gallica were selected in this assay because of their high activities of lignocellulolytic enzymes and the enzyme peaks appeared at the early stage of liquid state fermentation.Solid state fe...Pleurotus sp2 and Trametes gallica were selected in this assay because of their high activities of lignocellulolytic enzymes and the enzyme peaks appeared at the early stage of liquid state fermentation.Solid state fermentation was also investigated for their abilities and behaviors of enzyme\|production.The capabilities and characteristics of the two strains in degrading biomass were studied.When \Pleurotus sp2\ was incubated in wheat straw powder containing the liquid medium of low\|nitrogen,no\|carbon and high inorganic salt,the activities of MnP and Lac reached the peaks on the tenth day,but the activities of hemicellulases reached the peak on the 40th day.\Pleurotus sp2\ caused 17.6 of biomass loss.When \T.gallica was incubated in wheat straw powder containing the liquid medium of hlig\|nitrogen,or low\|nitrogen,no\|carbon and high inorganic salt,the activities of MnP reached the peaks on the tenth day,the lac and hemicelluloses on the 40th day,and the lignin peroxidases reached the peaks on the 50th day,and it caused more than 64% of biomass loss.Among them the hemicellulose was degraded by 71.96%,and the cellulose 66.21%. T.gallica% was very capable of degrading lignin of wheat straw and caused 34.37 loss during 20 days,46.71 loss during 30 days and 70.14% loss during 60 days.It was interesting that \T.gallica \degraded lignin preferentially with respect to cellulose,which was very beneficial to biopulping of paper industry.展开更多
采用多种筛选相结合的方法,从土壤、秸秆、牛粪等不同来源的样品中分离得到3株具有木质纤维素降解能力的细菌,其同时具有使堆肥快速升温和有效除臭潜力。经菌体形态观察、生理生化分析及16S r DNA鉴定,此3株细菌均属于Bacillus sp.。堆...采用多种筛选相结合的方法,从土壤、秸秆、牛粪等不同来源的样品中分离得到3株具有木质纤维素降解能力的细菌,其同时具有使堆肥快速升温和有效除臭潜力。经菌体形态观察、生理生化分析及16S r DNA鉴定,此3株细菌均属于Bacillus sp.。堆肥功能验证试验结果表明,添加此3株功能菌可使堆肥中纤维素、半纤维素和木质素的降解率分别比对照提高31.31%、19.57%和14.33%;堆肥结束时的发芽率达到93.3%,高出对照36.6%。此3株功能细菌具有很好的促进堆肥中木质纤维素降解和提高堆肥腐熟度的能力,可以考虑将其应用于较大规模的牛粪或其他畜禽粪便的堆肥处理。展开更多
文摘Pleurotus sp2 and Trametes gallica were selected in this assay because of their high activities of lignocellulolytic enzymes and the enzyme peaks appeared at the early stage of liquid state fermentation.Solid state fermentation was also investigated for their abilities and behaviors of enzyme\|production.The capabilities and characteristics of the two strains in degrading biomass were studied.When \Pleurotus sp2\ was incubated in wheat straw powder containing the liquid medium of low\|nitrogen,no\|carbon and high inorganic salt,the activities of MnP and Lac reached the peaks on the tenth day,but the activities of hemicellulases reached the peak on the 40th day.\Pleurotus sp2\ caused 17.6 of biomass loss.When \T.gallica was incubated in wheat straw powder containing the liquid medium of hlig\|nitrogen,or low\|nitrogen,no\|carbon and high inorganic salt,the activities of MnP reached the peaks on the tenth day,the lac and hemicelluloses on the 40th day,and the lignin peroxidases reached the peaks on the 50th day,and it caused more than 64% of biomass loss.Among them the hemicellulose was degraded by 71.96%,and the cellulose 66.21%. T.gallica% was very capable of degrading lignin of wheat straw and caused 34.37 loss during 20 days,46.71 loss during 30 days and 70.14% loss during 60 days.It was interesting that \T.gallica \degraded lignin preferentially with respect to cellulose,which was very beneficial to biopulping of paper industry.
文摘采用多种筛选相结合的方法,从土壤、秸秆、牛粪等不同来源的样品中分离得到3株具有木质纤维素降解能力的细菌,其同时具有使堆肥快速升温和有效除臭潜力。经菌体形态观察、生理生化分析及16S r DNA鉴定,此3株细菌均属于Bacillus sp.。堆肥功能验证试验结果表明,添加此3株功能菌可使堆肥中纤维素、半纤维素和木质素的降解率分别比对照提高31.31%、19.57%和14.33%;堆肥结束时的发芽率达到93.3%,高出对照36.6%。此3株功能细菌具有很好的促进堆肥中木质纤维素降解和提高堆肥腐熟度的能力,可以考虑将其应用于较大规模的牛粪或其他畜禽粪便的堆肥处理。
