Objective:To investigate the influence of xylooligosaccharides on skin inflammation,behavioral characteristics,neurotransmitters,and gut flora in a mouse model of atopic dermatitis(AD)induced by 2,4-dinitrofluorobenze...Objective:To investigate the influence of xylooligosaccharides on skin inflammation,behavioral characteristics,neurotransmitters,and gut flora in a mouse model of atopic dermatitis(AD)induced by 2,4-dinitrofluorobenzene(DNFB).Methods:The AD mouse model was created by administration of DNFB for 14 consecutive days.The scoring atopic dermatitis index,enzyme-linked immunosorbent assay(ELISA),histopathology,and immunohistochemical analyses were used to assess inflammation and depression-like behaviors.Furthermore,high-throughput 16S rRNA gene sequencing was used to determine the composition of fecal microbiota.Results:Xylooligosaccharides treatment reduced the number of scratches and skin thickness,mast cell infiltration and the levels of immunoglobulin(Ig)E and T-helper cytokines compared with the AD model group.Meanwhile,xylooligosaccharides treatment reduced the immobility time of mice in the forced swimming test and increased the total movement distance and movement distance in the center area in the open-field test.Furthermore,5-hydroxytryptamine and dopamine expression in the brain was increased following xylooligosaccharides treatment.Using network pharmacology,Gene Ontology analysis showed that the targets were mainly enriched in phosphatase binding and the regulation of leukocyte differentiation,which ameliorated AD mainly through the hypoxia inducible factor-1 and phosphatidylinositide 3-kinase-protein kinase B pathways.16S rRNA gene sequencing,diversity indices,and gut microbial taxonomic composition analysis showed DNFB-induced changes in intestinal microbiota diversity in AD mice.Comparative analysis indicated that xylooligosaccharides intake improved the gut microbiome by dramatically enhancing the concentration of Lactobacillus while decreasing the concentration of Bacteroides in mice.Conclusion:Xylooligosaccharides reduce inflammatory dermatosis and related depression-like behaviors via regulating intestinal homeostasis,having medicinal value as a nutritional and functional ingredient.展开更多
A computer program called Matrix Generator (MG) was developed for transforming sized DNA fragments into a presence/absence data matrix. Dynamic computation was run to avoid errors introduced using fixed-bin-width arit...A computer program called Matrix Generator (MG) was developed for transforming sized DNA fragments into a presence/absence data matrix. Dynamic computation was run to avoid errors introduced using fixed-bin-width arithmetic. MG can be used with bin sized fragments from AFLP, ISSR, RAPD, RFLP, and other molecular markers. The accuracy of MG was tested using fAFLP data of Abelia and the results show that MG results in higher resolution of taxa and is more reliable than programs of the similar usage.展开更多
Deep dielectric charging/discharging,caused by high energy electrons,is an important consideration in electronic devices used in space environments because it can lead to spacecraft anomalies and failures.The Jovian p...Deep dielectric charging/discharging,caused by high energy electrons,is an important consideration in electronic devices used in space environments because it can lead to spacecraft anomalies and failures.The Jovian planets,including Saturn,Uranus,Neptune and Jupiter’s moons,are believed to have robust electron radiation belts at relativistic energies.In particular,Jupiter is thought to have caused at least 42 internal electrostatic discharge events during the Voyager 1 flyby.With the development of deep space exploration,there is an increased focus on the deep dielectric charging effects in the orbits of Jovian planets.In this paper,GEANT4,a Monte Carlo toolkit,and radiation-induced conductivity(RIC)are used to calculate deep dielectric charging effects for Jovian planets.The results are compared with the criteria for preventing deep dielectric charging effects in Earth orbit.The findings show that effective criteria used in Earth orbit are not always appropriate for preventing deep dielectric charging effects in Jovian orbits.Generally,Io,Europa,Saturn(R_S=6),Uranus(L=4.73)and Ganymede missions should have a thicker shield or higher dielectric conductivity,while Neptune(L=7.4)and Callisto missions can have a thinner shield thickness or a lower dielectric conductivity.Moreover,dielectrics grounded with double metal layers and thinner dielectrics can also decrease the likelihood of discharges.展开更多
A new method using high water content material to mechanically fill cross roadways to form artificial bottom for coal faces was introduced. The reasonable determination of filling range, the optimization of the compou...A new method using high water content material to mechanically fill cross roadways to form artificial bottom for coal faces was introduced. The reasonable determination of filling range, the optimization of the compounding ratio of high water content material, and the filling technique were discussed in detail. This new method has been spread after industrial testing in Baodian Colliery. Compared with the traditional method, the manual wooden chock method, the new one decreases about 40% of the filling range and cost in dealing every one set of cross roadway in the testing condition.展开更多
In order to explore a technology for producing organic fertilizer by composting urban forest litter and river sediment passivated by fly ash, an experiment was conducted to study dynamic changes of several parameters ...In order to explore a technology for producing organic fertilizer by composting urban forest litter and river sediment passivated by fly ash, an experiment was conducted to study dynamic changes of several parameters including temperature, pH, organic matter, C/N and germination index (GI) during high-temperature composting of urban forest litter and river sediment at five different proportions (1:1 (Ⅰ), 1:2 (Ⅱ), 1:3 (Ⅲ), 2:1 (Ⅳ) and 3:1 (Ⅴ)). Results showed that the temperature and pH increased first and then decreased; at the proportion of 3:1, the temperature increased rapidly and the high-temperature duration was longest (5 d); at the end of the composting, all the treatments reached the decomposed status, the pH ranged from 7.47 to 8.87, and the organic matter content was reduced by 36%, 38%, 42%, 33% and 29%, respectively, indicating that increasing the proportion of urban forest litter was conducive to reducing the loss of organic matter. Due to low C/N ratio of river sediment, increasing the proportion of urban forest litter was helpful to improve the efficiency of composting. The GI of treatments I, IV and V reached 80% on day 26, 18 and 19 respectively, but the GI of treatments II and III did not reach this level until the end of composting. Considering the quality of fertilizer and efficiency of composting for large-scale production, the proportion between urban forest litter and river sediment is suitable to be set as 3:1 for production of organic fertilizer.展开更多
The seedling population structure of Pteleopsis suberosa and their regeneration mechanisms were investigated in four roadside environments (graded, adjacent, intermediate and ungraded areas) along paved and unpaved ...The seedling population structure of Pteleopsis suberosa and their regeneration mechanisms were investigated in four roadside environments (graded, adjacent, intermediate and ungraded areas) along paved and unpaved roads in West Africa. A total of 203 quadrats of 2 m × 5 m in size were surveyed and placed along transects parallel to the roads. Within each quadrat, the total number of seedlings and the number of living shoots per seedling base were recorded. Regeneration mechanisms were determined by assessing basal and aerial sprouts and excavating the root systems below ground level. The results show that the total seedling density and the densities of single- and multi-stemmed individuals varied significantly (p 〈 0.05) among the four roadside environments. However, all seedlings were produced asexually; root suckers were predominant (98%) compared to water sprout (1%) and coppices (less than 1%). This study demonstrates that an intermediate level of soil disturbance from grading along paved and unpaved roads may stimulate P. suberosa regeneration by root suck- ering. Road type (paved and unpaved) did not affect seedling density, but was a highly significant variable in relation to the coppicing ability of P. suberosa populations in roadside sites. In conclusion, P. suberosa is a disturbance-tolerant species which can proliferate mainly by root suckering after roadwork disturbance.展开更多
AIM: To investigate the action of genistein (GST), a broad spectrum tyrosine kinase inhibitor, on voltagegated potassium channels in guinea pig proximal colon smooth muscle cells. METHODS: Smooth muscle cells in g...AIM: To investigate the action of genistein (GST), a broad spectrum tyrosine kinase inhibitor, on voltagegated potassium channels in guinea pig proximal colon smooth muscle cells. METHODS: Smooth muscle cells in guinea pig proximal colon were enzymatically isolated. Nystatin-perforated whole cell patch clamp technique was used to record potassium currents including fast transient outward current (Ikto) and delayed rectifier current (Ikdr), tWO of which were isolated pharmacologically with 10 mmol/L tetraethylammonium or 5 mmol/L 4-aminopyridine. Contamination of calcium-dependent potassium currents was minimized with no caldum and 0.2 mmol/L CdCl2 in an external solution. RESULTS: GST (10-100 μmol/L) reversibly and dosedependently reduced the peak amplitude of Ikto with an IC50 value of 22.0±6.9 μmol/L. To a lesser extent, Ikdr, was also inhibited in both peak current and sustained current. GST could not totally block the outward potassium current as a fraction of the outWard potassium current, which was insensitive to GST. GST had no effect on the steady-state activation (n =6) and inactivation kinetics (n =6) of Ikto. Sodium orthovanadate (1 retool/L), a potent inhibitor of tyrosine phosphatase, significantly inhibited GST-induced inhibition (P〈 0.05). CONCLUSION: GST can dose-dependently and reversibly block voltage-gated potassium channels in guinea pig proximal colon smooth muscle cells.展开更多
Studied the changes of heat physical parameters, gas concentration of O_2, CO,CH_4 and H_2, and temperature through full scale wood fire experiments in a tunnel and simulationby FLUNET, a kind of computational fluid d...Studied the changes of heat physical parameters, gas concentration of O_2, CO,CH_4 and H_2, and temperature through full scale wood fire experiments in a tunnel and simulationby FLUNET, a kind of computational fluid dynamics software.The results showthat the wood fire experiment and simulation are both oxygen-enriched combustions,which show that the simulation results coincide with the experiment.The results also provethat it is difficult for oxygen-enriched combustion to cause secondary disasters.Somesuggestions were put forward to prevent the occurrence of secondary disasters effectively.展开更多
It is recognised that the blockage of culverts by woody debris can result in an increased risk of infrastructure damage and flooding.To date,debris transport analysis has focused on regional fluvial systems and large ...It is recognised that the blockage of culverts by woody debris can result in an increased risk of infrastructure damage and flooding.To date,debris transport analysis has focused on regional fluvial systems and large woody debris,both in flume and field experiments.Given the social and economic risk associated with urban flooding,and as urban drainage design shifts away from subsurface piped network reliance,there is an increasing need to understand debris movement in urban watercourses.The prediction of urban watercourse small woody debris(SWD)movement,both quantity and risk,has undergone only limited analysis predominantly due to lack of field data.This paper describes the development of a methodology to enable the collection of accurate and meaningful SWD residency and transportation data from watercourses.The presented research examines the limitations and effective function of PIT tag technology to collect SWD transport data in the field appropriate for risk and prediction analysis.Passive integrated transponder(PIT)technology provides a method to collect debris transport data within the urban environment.In this study,the tags are installed within small woody debris and released at known locations into a small urban natural watercourse enabling monitoring of movement and travel time.SWD velocity and detention are collated with solute time of travel,watercourse and point flow characteristics to identify the relationships between these key variables.The work presented tests three hypotheses:firstly,that the potential for unobstructed or un-detained SWD movement increases with flow velocity and water level.Secondly,that SWD travel distance,and the resistance forces along this travel path,influence SWD transport potential.Thirdly,the relationship between SWD and channel dimensions is examined with the aim of advancing representative debris transport prediction modelling.展开更多
The detailed structures of the volcanic edifice and plumbing system of the early Permian Tarim flood basalt were investigated by three-dimensional seismic imaging. The images show that the Tarim flood basalt erupted f...The detailed structures of the volcanic edifice and plumbing system of the early Permian Tarim flood basalt were investigated by three-dimensional seismic imaging. The images show that the Tarim flood basalt erupted from central volcanoes distributed along major faults. The uppermost magma formed a single lava lobe with a volume of *0.74 km3 and an aspect ratio of 50,implying a sheet flow eruption with a high effusion rate. At the subsurface level, all central volcanoes, irrespective of size, had a separate pipe-like feeder vent. Only thirteen dikes were identified at shallow depths of \100 m below the lava surface; deeper dikes were even rarer. The pipelike plumbing system and the paucity of dilational dikes were different from the typical plumbing system of flood basalt provinces on Earth, which are normally buoyancycontrolled dike–sill networks. A gas-driven vigorous eruption can be inferred from the imaged structure, which means that the Tarim flood basalt may have carried and released a large proportion of volcanic gas, similar to some mafic volcaniclastic deposit-bearing continental flood basalt provinces.展开更多
文摘Objective:To investigate the influence of xylooligosaccharides on skin inflammation,behavioral characteristics,neurotransmitters,and gut flora in a mouse model of atopic dermatitis(AD)induced by 2,4-dinitrofluorobenzene(DNFB).Methods:The AD mouse model was created by administration of DNFB for 14 consecutive days.The scoring atopic dermatitis index,enzyme-linked immunosorbent assay(ELISA),histopathology,and immunohistochemical analyses were used to assess inflammation and depression-like behaviors.Furthermore,high-throughput 16S rRNA gene sequencing was used to determine the composition of fecal microbiota.Results:Xylooligosaccharides treatment reduced the number of scratches and skin thickness,mast cell infiltration and the levels of immunoglobulin(Ig)E and T-helper cytokines compared with the AD model group.Meanwhile,xylooligosaccharides treatment reduced the immobility time of mice in the forced swimming test and increased the total movement distance and movement distance in the center area in the open-field test.Furthermore,5-hydroxytryptamine and dopamine expression in the brain was increased following xylooligosaccharides treatment.Using network pharmacology,Gene Ontology analysis showed that the targets were mainly enriched in phosphatase binding and the regulation of leukocyte differentiation,which ameliorated AD mainly through the hypoxia inducible factor-1 and phosphatidylinositide 3-kinase-protein kinase B pathways.16S rRNA gene sequencing,diversity indices,and gut microbial taxonomic composition analysis showed DNFB-induced changes in intestinal microbiota diversity in AD mice.Comparative analysis indicated that xylooligosaccharides intake improved the gut microbiome by dramatically enhancing the concentration of Lactobacillus while decreasing the concentration of Bacteroides in mice.Conclusion:Xylooligosaccharides reduce inflammatory dermatosis and related depression-like behaviors via regulating intestinal homeostasis,having medicinal value as a nutritional and functional ingredient.
文摘A computer program called Matrix Generator (MG) was developed for transforming sized DNA fragments into a presence/absence data matrix. Dynamic computation was run to avoid errors introduced using fixed-bin-width arithmetic. MG can be used with bin sized fragments from AFLP, ISSR, RAPD, RFLP, and other molecular markers. The accuracy of MG was tested using fAFLP data of Abelia and the results show that MG results in higher resolution of taxa and is more reliable than programs of the similar usage.
基金supported by Beijing Municipal Natural Science Foundation-Quantitative Research on Mitigating Deep Dielectric Charging Effects in Jupiter orbits(No.3184048)National Key Scientific Instrument and Equipment Development Projects,China(No.2012YQ03014207)。
文摘Deep dielectric charging/discharging,caused by high energy electrons,is an important consideration in electronic devices used in space environments because it can lead to spacecraft anomalies and failures.The Jovian planets,including Saturn,Uranus,Neptune and Jupiter’s moons,are believed to have robust electron radiation belts at relativistic energies.In particular,Jupiter is thought to have caused at least 42 internal electrostatic discharge events during the Voyager 1 flyby.With the development of deep space exploration,there is an increased focus on the deep dielectric charging effects in the orbits of Jovian planets.In this paper,GEANT4,a Monte Carlo toolkit,and radiation-induced conductivity(RIC)are used to calculate deep dielectric charging effects for Jovian planets.The results are compared with the criteria for preventing deep dielectric charging effects in Earth orbit.The findings show that effective criteria used in Earth orbit are not always appropriate for preventing deep dielectric charging effects in Jovian orbits.Generally,Io,Europa,Saturn(R_S=6),Uranus(L=4.73)and Ganymede missions should have a thicker shield or higher dielectric conductivity,while Neptune(L=7.4)and Callisto missions can have a thinner shield thickness or a lower dielectric conductivity.Moreover,dielectrics grounded with double metal layers and thinner dielectrics can also decrease the likelihood of discharges.
文摘A new method using high water content material to mechanically fill cross roadways to form artificial bottom for coal faces was introduced. The reasonable determination of filling range, the optimization of the compounding ratio of high water content material, and the filling technique were discussed in detail. This new method has been spread after industrial testing in Baodian Colliery. Compared with the traditional method, the manual wooden chock method, the new one decreases about 40% of the filling range and cost in dealing every one set of cross roadway in the testing condition.
基金Supported by National Spark Program of China(2010GA781004)Shenzhen Science and Technology Plan Project(CXZZ20140422142833835,CXZZ20150527171538718,GCZX2015051514435234)~~
文摘In order to explore a technology for producing organic fertilizer by composting urban forest litter and river sediment passivated by fly ash, an experiment was conducted to study dynamic changes of several parameters including temperature, pH, organic matter, C/N and germination index (GI) during high-temperature composting of urban forest litter and river sediment at five different proportions (1:1 (Ⅰ), 1:2 (Ⅱ), 1:3 (Ⅲ), 2:1 (Ⅳ) and 3:1 (Ⅴ)). Results showed that the temperature and pH increased first and then decreased; at the proportion of 3:1, the temperature increased rapidly and the high-temperature duration was longest (5 d); at the end of the composting, all the treatments reached the decomposed status, the pH ranged from 7.47 to 8.87, and the organic matter content was reduced by 36%, 38%, 42%, 33% and 29%, respectively, indicating that increasing the proportion of urban forest litter was conducive to reducing the loss of organic matter. Due to low C/N ratio of river sediment, increasing the proportion of urban forest litter was helpful to improve the efficiency of composting. The GI of treatments I, IV and V reached 80% on day 26, 18 and 19 respectively, but the GI of treatments II and III did not reach this level until the end of composting. Considering the quality of fertilizer and efficiency of composting for large-scale production, the proportion between urban forest litter and river sediment is suitable to be set as 3:1 for production of organic fertilizer.
基金Swedish International Development Cooperation Agency (Sida).
文摘The seedling population structure of Pteleopsis suberosa and their regeneration mechanisms were investigated in four roadside environments (graded, adjacent, intermediate and ungraded areas) along paved and unpaved roads in West Africa. A total of 203 quadrats of 2 m × 5 m in size were surveyed and placed along transects parallel to the roads. Within each quadrat, the total number of seedlings and the number of living shoots per seedling base were recorded. Regeneration mechanisms were determined by assessing basal and aerial sprouts and excavating the root systems below ground level. The results show that the total seedling density and the densities of single- and multi-stemmed individuals varied significantly (p 〈 0.05) among the four roadside environments. However, all seedlings were produced asexually; root suckers were predominant (98%) compared to water sprout (1%) and coppices (less than 1%). This study demonstrates that an intermediate level of soil disturbance from grading along paved and unpaved roads may stimulate P. suberosa regeneration by root suck- ering. Road type (paved and unpaved) did not affect seedling density, but was a highly significant variable in relation to the coppicing ability of P. suberosa populations in roadside sites. In conclusion, P. suberosa is a disturbance-tolerant species which can proliferate mainly by root suckering after roadwork disturbance.
文摘AIM: To investigate the action of genistein (GST), a broad spectrum tyrosine kinase inhibitor, on voltagegated potassium channels in guinea pig proximal colon smooth muscle cells. METHODS: Smooth muscle cells in guinea pig proximal colon were enzymatically isolated. Nystatin-perforated whole cell patch clamp technique was used to record potassium currents including fast transient outward current (Ikto) and delayed rectifier current (Ikdr), tWO of which were isolated pharmacologically with 10 mmol/L tetraethylammonium or 5 mmol/L 4-aminopyridine. Contamination of calcium-dependent potassium currents was minimized with no caldum and 0.2 mmol/L CdCl2 in an external solution. RESULTS: GST (10-100 μmol/L) reversibly and dosedependently reduced the peak amplitude of Ikto with an IC50 value of 22.0±6.9 μmol/L. To a lesser extent, Ikdr, was also inhibited in both peak current and sustained current. GST could not totally block the outward potassium current as a fraction of the outWard potassium current, which was insensitive to GST. GST had no effect on the steady-state activation (n =6) and inactivation kinetics (n =6) of Ikto. Sodium orthovanadate (1 retool/L), a potent inhibitor of tyrosine phosphatase, significantly inhibited GST-induced inhibition (P〈 0.05). CONCLUSION: GST can dose-dependently and reversibly block voltage-gated potassium channels in guinea pig proximal colon smooth muscle cells.
基金Supported by the National"Eleventh Five-Year"Key Scientific and Technological Support Program Project(2007BAK22B04)2008 Independent Task(SKLCRSM08B12)
文摘Studied the changes of heat physical parameters, gas concentration of O_2, CO,CH_4 and H_2, and temperature through full scale wood fire experiments in a tunnel and simulationby FLUNET, a kind of computational fluid dynamics software.The results showthat the wood fire experiment and simulation are both oxygen-enriched combustions,which show that the simulation results coincide with the experiment.The results also provethat it is difficult for oxygen-enriched combustion to cause secondary disasters.Somesuggestions were put forward to prevent the occurrence of secondary disasters effectively.
基金supported by the Engineering and Physical Sciences Research Council(Grant Nos.EPSRC EP/J501335/1 and EP/K50337X/1)the Heriot-Watt University School of the Built Environment
文摘It is recognised that the blockage of culverts by woody debris can result in an increased risk of infrastructure damage and flooding.To date,debris transport analysis has focused on regional fluvial systems and large woody debris,both in flume and field experiments.Given the social and economic risk associated with urban flooding,and as urban drainage design shifts away from subsurface piped network reliance,there is an increasing need to understand debris movement in urban watercourses.The prediction of urban watercourse small woody debris(SWD)movement,both quantity and risk,has undergone only limited analysis predominantly due to lack of field data.This paper describes the development of a methodology to enable the collection of accurate and meaningful SWD residency and transportation data from watercourses.The presented research examines the limitations and effective function of PIT tag technology to collect SWD transport data in the field appropriate for risk and prediction analysis.Passive integrated transponder(PIT)technology provides a method to collect debris transport data within the urban environment.In this study,the tags are installed within small woody debris and released at known locations into a small urban natural watercourse enabling monitoring of movement and travel time.SWD velocity and detention are collated with solute time of travel,watercourse and point flow characteristics to identify the relationships between these key variables.The work presented tests three hypotheses:firstly,that the potential for unobstructed or un-detained SWD movement increases with flow velocity and water level.Secondly,that SWD travel distance,and the resistance forces along this travel path,influence SWD transport potential.Thirdly,the relationship between SWD and channel dimensions is examined with the aim of advancing representative debris transport prediction modelling.
基金supported by the National Basic Research Program of China(2010CB808906)the National Natural Science Foundation of China(41272368)to WT
文摘The detailed structures of the volcanic edifice and plumbing system of the early Permian Tarim flood basalt were investigated by three-dimensional seismic imaging. The images show that the Tarim flood basalt erupted from central volcanoes distributed along major faults. The uppermost magma formed a single lava lobe with a volume of *0.74 km3 and an aspect ratio of 50,implying a sheet flow eruption with a high effusion rate. At the subsurface level, all central volcanoes, irrespective of size, had a separate pipe-like feeder vent. Only thirteen dikes were identified at shallow depths of \100 m below the lava surface; deeper dikes were even rarer. The pipelike plumbing system and the paucity of dilational dikes were different from the typical plumbing system of flood basalt provinces on Earth, which are normally buoyancycontrolled dike–sill networks. A gas-driven vigorous eruption can be inferred from the imaged structure, which means that the Tarim flood basalt may have carried and released a large proportion of volcanic gas, similar to some mafic volcaniclastic deposit-bearing continental flood basalt provinces.