A new lignan, together with a known one, was isolated from Isodon lophanthoides var. gerardianus [Bentham] H. Hara. The structure of the new lignan was elucidated as 1_acetoxyl_2e_piperonyl_6e_[6_methoxyl_pi...A new lignan, together with a known one, was isolated from Isodon lophanthoides var. gerardianus [Bentham] H. Hara. The structure of the new lignan was elucidated as 1_acetoxyl_2e_piperonyl_6e_[6_methoxyl_piperonyl]_3,7_dioxabicyclo_[3,3,0]_octane mainly by 1D and 2D NMR techniques.展开更多
Two new coumarins, named marminal (13) and 7' O methylmarmin (15), along with nineteen known compounds: ( sitosteryl pentadecanoate (1), 4 methoxy 1 methyl 2 quinolone (2), aurapten (3), 4 sitosten 3 on...Two new coumarins, named marminal (13) and 7' O methylmarmin (15), along with nineteen known compounds: ( sitosteryl pentadecanoate (1), 4 methoxy 1 methyl 2 quinolone (2), aurapten (3), 4 sitosten 3 one (4), lupeol (5), imperatorin (6), xanthotoxin (7), dictamnine (8), (+) epoxyaurapten (9), ( sitosterol (10), ( fagarine (11), skimmianine (12), scoparone (14), umbelliferone (16), scopoletin (17), decursinol (18), marmesin (19), marmin (20), and integriquinolone (21) were isolated from the methanolic extract of the roots of Aegle marmelos Corr. (family Rutaceae).展开更多
The hydroxyl radical（-OH）-scavenging ability of culture filtrates from submerged culture of Lentinula edodes AX3 and five mildews on lignocellulose substance was analysed. Only L. edodes AX3 showed significant -OH-s...The hydroxyl radical（-OH）-scavenging ability of culture filtrates from submerged culture of Lentinula edodes AX3 and five mildews on lignocellulose substance was analysed. Only L. edodes AX3 showed significant -OH-scavenging ability which reached 52.2% at about the 48th hour. All mildews could hardly scavenge -OH under the experimental conditions. -OH-scavenging ability is considered related to the mode and ability of lignocellulose degradation of a strain. The degradation or bioconversion products might be the substance base for.this effect.展开更多
Research on selection of superior breeding infraspecies Gyrinops versteegii （Gilg） Domke aims to select an infraspecies of G. versteegii having internal factors that affect the quality and quantity of production aro...Research on selection of superior breeding infraspecies Gyrinops versteegii （Gilg） Domke aims to select an infraspecies of G. versteegii having internal factors that affect the quality and quantity of production aromatic resin or agarwood. The internal factors are variations in wood anatomy, phytochemicals and agarwood of infraspecies G. versteegii. Of five local populations taken from West Lombok Island in Indonesia, the research areas are geographically located at coordinates： 8°15′-8°40′ South Latitude and 116°00′-116°20′East Longitude. Quality and quantity of agarwood production is influenced by internal factors of eaglewood trees, such as high starch production and the size of the cells making up the larger wood tissue. Infraspecies G. versteegii that have quality and quantity production of aromatic resin （the best agarwood） is Beringin group and the highest quantity of agarwood production （agarwood formation on the whole organ） is Soyun group.展开更多
Stroke has been a common problem in the global public health because of its high incidence,recur-rence,disability rate and mortality,among which ischemic stroke accounts for about 80%of the total stroke.Cerebral ische...Stroke has been a common problem in the global public health because of its high incidence,recur-rence,disability rate and mortality,among which ischemic stroke accounts for about 80%of the total stroke.Cerebral ischemia reperfusion injury(CIRI)is one of the most important pathological injuries after ischemic stroke,and the effective treatment has always been a research focus at home and abroad.The proposal of the concept of neurovascular unit(NVU)which extends treatment ideas from local to holistic is in accordance with the holistic concept and treatment based on syndrome differentiation in Chinese medicine.Chinese medicine has many advantages,such as being multicomponent,multitarget,and multipath while stem cells are effective biological therapeutic means,both of which have been proved to exert comprehensive and definite effects on NVU injury caused by CIRI.This artide reviews the molecular mechanisms of NVU injury and the cerebropro-tective effects of Chinese medicine monomers or compound formulas combined with MSCs on NUV injury after cerebral ischemia,with a view to provide a theoretical basis for future studies.展开更多
An efficient method for the simultaneous quantification of three flavone aglycones was developed and validated for the control of flavonoids in Flos Chrysanthemi （FC）. The method employed mixed solvent of hydrochlor...An efficient method for the simultaneous quantification of three flavone aglycones was developed and validated for the control of flavonoids in Flos Chrysanthemi （FC）. The method employed mixed solvent of hydrochloric acid （HC1） and methanol to extract and hydrolyze flavone glycosides simultaneously from powdered FC followed by HPLC to determine the contents of three flavone aglycones, luteolin, apigenin and diosmetin, which are the major bioactive flavones in FC. The extraction and hydrolysis conditions optimized by the orthogonal tests were as follows： powdered FC was refluxed in 80% methanol （v/v） containing 2.4 M HC1 for 2 h in 80 ℃ water bath. The HPLC separation was performed on C18 column and detected with DAD at 344 nm. The calibration curves for luteolin, apigenin and diosmetin were linear over the ranges of 2.233-71.46 μg/mL, 1.668-53.38 μg/mL and 1.410-45.11 μg/mL, their assay recoveries were 100.3%, 103.9% and 100.4%, their intra-day repeatability （R.S.D.） were 0.68%, 0.21% and 0.38% and their inter-day repeatability were 2.6%, 1.7% and 2.0%, respectively. The developed method has been successfully utilized to assay eight species of popular FC samples, especially to analyze Hangbaiju, the well-known FC in China.展开更多
To investigate the chemical constituents of the stems ofAquilaria sinensis （Lour.） Gilg, the separation and purification were performed by solvent extraction, repeated chromatography on silica gel, Sephadex LH-20 an...To investigate the chemical constituents of the stems ofAquilaria sinensis （Lour.） Gilg, the separation and purification were performed by solvent extraction, repeated chromatography on silica gel, Sephadex LH-20 and prep-HPLC. The structures were determined by spectrum analysis. Thirteen known compounds were isolated and their structures were identified as justicidin A （1）, justidin F （2）, ciwujiatone （3）, （＋） syringaresinol （4）, syringaresinol-4,4＇-di-O-13-D-glucopyranoside （5）, syringaresinol-4＂O-β-D- glucopyranoside （6）, curuilignan D （7）, syringin （8）, koaburaside （9）, 3,4,5-trimethoxyphenyl-l-O-β-D-glucopyranoside （10）, 3,4,5-trimethoxyphenyl-l-O-13-D-apiofuranosyl-（1 ＂→6＇） glucopyranoside （11）, 7-ketositosterol （12）, 7-oxo-5,6-dihydrostigmasterol （13）. All the compounds described above were isolated from genus Aquilaria for the first time.展开更多
In the present study,we aimed to investigate the chemical constituents from Solanum lyratum.The constituents were separated by column chromatography on silica gel,Sephadex LH-20 and preparative HPLC.Their structures w...In the present study,we aimed to investigate the chemical constituents from Solanum lyratum.The constituents were separated by column chromatography on silica gel,Sephadex LH-20 and preparative HPLC.Their structures were elucidated by spectroscopic means.A total of 13 compounds were isolated from S.lyraturn and identified as syringin(1),(+)-isolariciresinol(2),(+)-syringaresinol(3),leptolepisol D(4),(-)-secoisolariciresinol(5),(-)-epi-syringaresinol(6),aviculin(7),zhebeiresinol(8),ciwujiatone(9),(-)-(7′S,8S,8′R)-4,4′-dihydroxy-3,3′,5,5′-tetramethoxy-7′,9-epoxylignan-9′-ol-7-one(10),(+)-lariciresinol(11),(+)-pinoresinol(12)and(+)-medioresinol(13).All the compounds were isolated from S.lyratum for the first time.展开更多
文摘A new lignan, together with a known one, was isolated from Isodon lophanthoides var. gerardianus [Bentham] H. Hara. The structure of the new lignan was elucidated as 1_acetoxyl_2e_piperonyl_6e_[6_methoxyl_piperonyl]_3,7_dioxabicyclo_[3,3,0]_octane mainly by 1D and 2D NMR techniques.
文摘The hydroxyl radical（-OH）-scavenging ability of culture filtrates from submerged culture of Lentinula edodes AX3 and five mildews on lignocellulose substance was analysed. Only L. edodes AX3 showed significant -OH-scavenging ability which reached 52.2% at about the 48th hour. All mildews could hardly scavenge -OH under the experimental conditions. -OH-scavenging ability is considered related to the mode and ability of lignocellulose degradation of a strain. The degradation or bioconversion products might be the substance base for.this effect.
文摘Research on selection of superior breeding infraspecies Gyrinops versteegii （Gilg） Domke aims to select an infraspecies of G. versteegii having internal factors that affect the quality and quantity of production aromatic resin or agarwood. The internal factors are variations in wood anatomy, phytochemicals and agarwood of infraspecies G. versteegii. Of five local populations taken from West Lombok Island in Indonesia, the research areas are geographically located at coordinates： 8°15′-8°40′ South Latitude and 116°00′-116°20′East Longitude. Quality and quantity of agarwood production is influenced by internal factors of eaglewood trees, such as high starch production and the size of the cells making up the larger wood tissue. Infraspecies G. versteegii that have quality and quantity production of aromatic resin （the best agarwood） is Beringin group and the highest quantity of agarwood production （agarwood formation on the whole organ） is Soyun group.
基金We thank for the funding support from the National Natural Science Foundation of China(8197151612)Henan Provincial Project of Outstanding Talents in Science and Technology Innovation(2018JR0006).
文摘Stroke has been a common problem in the global public health because of its high incidence,recur-rence,disability rate and mortality,among which ischemic stroke accounts for about 80%of the total stroke.Cerebral ischemia reperfusion injury(CIRI)is one of the most important pathological injuries after ischemic stroke,and the effective treatment has always been a research focus at home and abroad.The proposal of the concept of neurovascular unit(NVU)which extends treatment ideas from local to holistic is in accordance with the holistic concept and treatment based on syndrome differentiation in Chinese medicine.Chinese medicine has many advantages,such as being multicomponent,multitarget,and multipath while stem cells are effective biological therapeutic means,both of which have been proved to exert comprehensive and definite effects on NVU injury caused by CIRI.This artide reviews the molecular mechanisms of NVU injury and the cerebropro-tective effects of Chinese medicine monomers or compound formulas combined with MSCs on NUV injury after cerebral ischemia,with a view to provide a theoretical basis for future studies.
基金The Ministry of Sciences and Technology of China (Grant No. 2006BAI06A18-11)Administration and Traditional Chinese Medicine,Zhejiang,China (Grant No. 2007ZA012)
文摘An efficient method for the simultaneous quantification of three flavone aglycones was developed and validated for the control of flavonoids in Flos Chrysanthemi （FC）. The method employed mixed solvent of hydrochloric acid （HC1） and methanol to extract and hydrolyze flavone glycosides simultaneously from powdered FC followed by HPLC to determine the contents of three flavone aglycones, luteolin, apigenin and diosmetin, which are the major bioactive flavones in FC. The extraction and hydrolysis conditions optimized by the orthogonal tests were as follows： powdered FC was refluxed in 80% methanol （v/v） containing 2.4 M HC1 for 2 h in 80 ℃ water bath. The HPLC separation was performed on C18 column and detected with DAD at 344 nm. The calibration curves for luteolin, apigenin and diosmetin were linear over the ranges of 2.233-71.46 μg/mL, 1.668-53.38 μg/mL and 1.410-45.11 μg/mL, their assay recoveries were 100.3%, 103.9% and 100.4%, their intra-day repeatability （R.S.D.） were 0.68%, 0.21% and 0.38% and their inter-day repeatability were 2.6%, 1.7% and 2.0%, respectively. The developed method has been successfully utilized to assay eight species of popular FC samples, especially to analyze Hangbaiju, the well-known FC in China.
基金The Special Program for New Drug Innovation of the Ministry of Science and Technology,China(Grant No. 2009ZX311-004,2009ZX0308-004)
文摘To investigate the chemical constituents of the stems ofAquilaria sinensis （Lour.） Gilg, the separation and purification were performed by solvent extraction, repeated chromatography on silica gel, Sephadex LH-20 and prep-HPLC. The structures were determined by spectrum analysis. Thirteen known compounds were isolated and their structures were identified as justicidin A （1）, justidin F （2）, ciwujiatone （3）, （＋） syringaresinol （4）, syringaresinol-4,4＇-di-O-13-D-glucopyranoside （5）, syringaresinol-4＂O-β-D- glucopyranoside （6）, curuilignan D （7）, syringin （8）, koaburaside （9）, 3,4,5-trimethoxyphenyl-l-O-β-D-glucopyranoside （10）, 3,4,5-trimethoxyphenyl-l-O-13-D-apiofuranosyl-（1 ＂→6＇） glucopyranoside （11）, 7-ketositosterol （12）, 7-oxo-5,6-dihydrostigmasterol （13）. All the compounds described above were isolated from genus Aquilaria for the first time.
基金Science and Technology Department Plan of Jilin Province,China(Grant No.20160101341JC and 20160622010JC)
文摘In the present study,we aimed to investigate the chemical constituents from Solanum lyratum.The constituents were separated by column chromatography on silica gel,Sephadex LH-20 and preparative HPLC.Their structures were elucidated by spectroscopic means.A total of 13 compounds were isolated from S.lyraturn and identified as syringin(1),(+)-isolariciresinol(2),(+)-syringaresinol(3),leptolepisol D(4),(-)-secoisolariciresinol(5),(-)-epi-syringaresinol(6),aviculin(7),zhebeiresinol(8),ciwujiatone(9),(-)-(7′S,8S,8′R)-4,4′-dihydroxy-3,3′,5,5′-tetramethoxy-7′,9-epoxylignan-9′-ol-7-one(10),(+)-lariciresinol(11),(+)-pinoresinol(12)and(+)-medioresinol(13).All the compounds were isolated from S.lyratum for the first time.
