Amphioctopus fangsiao is one of the most economically important species and has been considered to be a candidate for aquaculture. In order to facilitate its fine-scale genetic analyses, we constructed a normalized fu...Amphioctopus fangsiao is one of the most economically important species and has been considered to be a candidate for aquaculture. In order to facilitate its fine-scale genetic analyses, we constructed a normalized full-length library successfully and developed a set of microsatellite markers in this study. The normalized full-length library had a storage capacity of 6.9×105 independent clones. The recombination efficiency was 95% and the average size of inserted fragments was longer than 1000 bp. A total of 3440 high quality ESTs were obtained, which were assembled into 1803 unigenes. Of these unigenes, 450(25%) were assigned into 33 Gene Ontology terms, 576(31.9%) into 153 Kyoto Encyclopedia of Genes and Genomes pathways, and 275(15.3%) into 22 Clusters of Orthologous Groups. Seventy-six polymorphic microsatellite markers were identified. The number of alleles per locus ranged from 4 to 17, and the observed and expected heterozygosities varied between 0.167 and 0.967 and between 0.326 and 0.944, respectively. Twelve loci were significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction and no linkage disequilibrium was found between different loci. This study provided not only a useful resource for the isolation of the functional genes, but also a set of informative microsatellites for the assessment of population structure and conservation genetics of A. fangsiao.展开更多
In this study, we looked at a method quantifying EEC （embodied energy and CO2） and the effect when we prolonged the building life time particularly through the durable improvement of the structure. Increasing the co...In this study, we looked at a method quantifying EEC （embodied energy and CO2） and the effect when we prolonged the building life time particularly through the durable improvement of the structure. Increasing the covering thickness of concrete for reinforcing bars and the earthquake-resistant strength are methods to increase the durability of the structure. The calculation method to obtain the quantity of concrete and reinforcing bars is provided. The EEC increase is evaluated from the 2005 input-output table in Japan. These results show that EE （embodied energy） in the construction phase is increased by 11% to 20% and EC （embodied CO2） 17% to 32%. However, annual EE is reduced 66% to 72% and EC 70% to 79%,展开更多
基金the National Natural Science Foundation of China (Nos. 31302215, 31272643)the Shandong Provincial Natural Science Foundation (Nos. BS2014NY010, ZR2013CQ030)the Shandong Provincial Primary Research and Development Projects (No. 2015GNC110017)
文摘Amphioctopus fangsiao is one of the most economically important species and has been considered to be a candidate for aquaculture. In order to facilitate its fine-scale genetic analyses, we constructed a normalized full-length library successfully and developed a set of microsatellite markers in this study. The normalized full-length library had a storage capacity of 6.9×105 independent clones. The recombination efficiency was 95% and the average size of inserted fragments was longer than 1000 bp. A total of 3440 high quality ESTs were obtained, which were assembled into 1803 unigenes. Of these unigenes, 450(25%) were assigned into 33 Gene Ontology terms, 576(31.9%) into 153 Kyoto Encyclopedia of Genes and Genomes pathways, and 275(15.3%) into 22 Clusters of Orthologous Groups. Seventy-six polymorphic microsatellite markers were identified. The number of alleles per locus ranged from 4 to 17, and the observed and expected heterozygosities varied between 0.167 and 0.967 and between 0.326 and 0.944, respectively. Twelve loci were significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction and no linkage disequilibrium was found between different loci. This study provided not only a useful resource for the isolation of the functional genes, but also a set of informative microsatellites for the assessment of population structure and conservation genetics of A. fangsiao.
文摘In this study, we looked at a method quantifying EEC （embodied energy and CO2） and the effect when we prolonged the building life time particularly through the durable improvement of the structure. Increasing the covering thickness of concrete for reinforcing bars and the earthquake-resistant strength are methods to increase the durability of the structure. The calculation method to obtain the quantity of concrete and reinforcing bars is provided. The EEC increase is evaluated from the 2005 input-output table in Japan. These results show that EE （embodied energy） in the construction phase is increased by 11% to 20% and EC （embodied CO2） 17% to 32%. However, annual EE is reduced 66% to 72% and EC 70% to 79%,
