Biaxial compression tests on plain concrete suffered high temperature ranging from 200 ℃ to 600 ℃ were carried out using the large-scale dynamic-static tri-axial concrete test system at the State Key Laboratory of C...Biaxial compression tests on plain concrete suffered high temperature ranging from 200 ℃ to 600 ℃ were carried out using the large-scale dynamic-static tri-axial concrete test system at the State Key Laboratory of Coastal and Offshore Engineering with designated stress ratios of 0, 0.25, 0.5, 0.75 and 1, respectively. The measured strength and strain were reported and the changes in both biaxial compressive failure envelopes and strains at peak stresses were analyzed. The regressive equation of initial elastic modulus in the biggest principal compressive stress direction was derived from test results. With the published results from previous biaxial tension-compression experiments, a three-parameter failure criterion has been proposed. A biaxial nonlinear elastic incremental constitutive model was developed for the compressive stress directions by using the equivalent uniaxial strain values deduced from test results. Analytical results obtained from the proposed biaxial constitutive model achieve good agreement with the experimental results.展开更多
The spatiotemporal relationships in high-resolution during odontogenesis remain poorly understood.We report a cell lineage and atlas of developing mouse teeth.We performed a large-scale(92,688 cells)single cell RNA se...The spatiotemporal relationships in high-resolution during odontogenesis remain poorly understood.We report a cell lineage and atlas of developing mouse teeth.We performed a large-scale(92,688 cells)single cell RNA sequencing,tracing the cell trajectories during odontogenesis from embryonic days 10.5 to 16.5.Combined with an assay for transposase-accessible chromatin with high-throughput sequencing,our results suggest that mesenchymal cells show the specific transcriptome profiles to distinguish the tooth types.Subsequently,we identified key gene regulatory networks in teeth and bone formation and uncovered spatiotemporal patterns of odontogenic mesenchymal cells.CD24^(+)and Plac8^(+)cells from the mesenchyme at the bell stage were distributed in the upper half and preodontoblast layer of the dental papilla,respectively,which could individually induce nonodontogenic epithelia to form tooth-like structures.Specifically,the Plac8^(+)tissue we discovered is the smallest piece with the most homogenous cells that could induce tooth regeneration to date.Our work reveals previously unknown heterogeneity and spatiotemporal patterns of tooth germs that may lead to tooth regeneration for regenerative dentistry.展开更多
基金Sponsored by the Science Foundation of Educational Department of Liaoning Province(Grant No.2023901023)
文摘Biaxial compression tests on plain concrete suffered high temperature ranging from 200 ℃ to 600 ℃ were carried out using the large-scale dynamic-static tri-axial concrete test system at the State Key Laboratory of Coastal and Offshore Engineering with designated stress ratios of 0, 0.25, 0.5, 0.75 and 1, respectively. The measured strength and strain were reported and the changes in both biaxial compressive failure envelopes and strains at peak stresses were analyzed. The regressive equation of initial elastic modulus in the biggest principal compressive stress direction was derived from test results. With the published results from previous biaxial tension-compression experiments, a three-parameter failure criterion has been proposed. A biaxial nonlinear elastic incremental constitutive model was developed for the compressive stress directions by using the equivalent uniaxial strain values deduced from test results. Analytical results obtained from the proposed biaxial constitutive model achieve good agreement with the experimental results.
基金supported by the National Key Research and Development Program of China Stem Cell and Translational Research,China(2017YFA0104800)the Research Funds from Health@InnoHK Program launched by Innovation Technology Commission of the Hong Kong SAR,China+4 种基金National Natural Science Foundation of China(81570944 and 92068201)Science and Technology Planning Project of Guangdong Province,China(2020B1212060052)High-level Hospital Construction Project(DFJHBF202110)Youth Innovation Promotion of the Chinese Academy of Sciences(2019348)Guangzhou Key Medical Disciplines(2021–2023)。
文摘The spatiotemporal relationships in high-resolution during odontogenesis remain poorly understood.We report a cell lineage and atlas of developing mouse teeth.We performed a large-scale(92,688 cells)single cell RNA sequencing,tracing the cell trajectories during odontogenesis from embryonic days 10.5 to 16.5.Combined with an assay for transposase-accessible chromatin with high-throughput sequencing,our results suggest that mesenchymal cells show the specific transcriptome profiles to distinguish the tooth types.Subsequently,we identified key gene regulatory networks in teeth and bone formation and uncovered spatiotemporal patterns of odontogenic mesenchymal cells.CD24^(+)and Plac8^(+)cells from the mesenchyme at the bell stage were distributed in the upper half and preodontoblast layer of the dental papilla,respectively,which could individually induce nonodontogenic epithelia to form tooth-like structures.Specifically,the Plac8^(+)tissue we discovered is the smallest piece with the most homogenous cells that could induce tooth regeneration to date.Our work reveals previously unknown heterogeneity and spatiotemporal patterns of tooth germs that may lead to tooth regeneration for regenerative dentistry.
