我国具有杀根结线虫活性的植物资源统计

统计了我国自20世纪90年代以来,在植物源杀根结线虫活性药剂研究领域已经进行过活性筛选的60多种植物资源,其中,具有显著杀根结线虫活性的植物共21科29种;以南方根结线虫为活性受体的有13科18种植物;以爪哇根结线虫为活性受体的有7科8种;以花生根结线虫为活性受体的有3科4种。展望了杀根结线虫活性植物资源筛选工作的发展趋势。

应用草甘膦对杨树杀根灭萌技术研究

本文介绍白城地区森防站等单位1985～1987年清除杨树人工林内间伐时残留的伐根,应用草甘膦药剂喷洒伐根萌条,杀根灭萌效果显著,通过试验得出最佳配方、最佳施药期,为大面积应用提供了依据。

朱砂莲果实化学成分杀南方根结线虫活性研究

为了解朱砂莲(Aristolochia tuberosa O.F.Liang et S.M.Huang)的化学成分,从其果实的甲醇提取物中分离得到4个化合物。通过波普数据分析,分别鉴定为马兜铃内酰胺W(1)、8-epidiosbulbin e acetate(2)、diosbulbin B(3)和β-sitosterol(4)。化合物1~3对南方根结线虫2龄幼虫具有不同程度的毒杀作用,尤其马兜铃内酰胺W(1)活性最好,其96 h后的LC50为119.94μg m L–1。马兜铃属植物具有开发为新型植物源杀根结线虫剂的潜在价值。

Nematicidal Activity of Ten Kinds of Plant Extract against Hirschmanniella spp.

[Objective] The nematicidal activity of plant extracts was investigated. We aimed to explore a kind of botanical pesticide to control Hirschmanniella spp. [Method] The Chuanxiong （root）, agrimony （whole plant）, cocklebur （grain）, basil （leaves）, ragweed （stems and leaves）, green onion （root）, garlic （tuber）, Yuzhu （leaves）, marshpeper smartweed （whole plant）, horseweed （aboveground stems and leaves） were dried, grinded and sieved. The powders were then extracted by ethanol at room temperature for 24 h. The extract solutions were filtered through fil- ter pater. The obtained plant extracts were then diluted to determine their nematici- dal activities. [Result] The Chuanxiong extract had the highest nematicidal activity, followed by cocklebur extract, agrimony extract, basil extract, garlic extract, ragweed extract. The horseweed extract had the lowest nematicidal activity. [Conclusion] The Chuanxiong and other plants were natural, and they had a good environmental compatibility. They could be developed as botanical pesticides against Hirschman- niella spp.

Agrobacterium tumefaciens-mediated transformation of rice with the spider insecticidal gene conferring resistance to leaffolder and striped stem borer

Immature embryos of rice varieties "Xiushui11" and "Chunjiang 11" precultured for 4d were infected and transformed by Agrobacterium tumefaciens strain EHA101/pExT7 (containing the spider insecticidal gene). The resistant cant were transferred onto the differentiation medium and plants were regenerated. The transformation frequency reached 56%-72% measured as numbers of Geneticin (G418)-resistant calli produced and 36%-60% measured as numbers of transgenic plants regenerated, respectively. PCR and Southern blot analysis of transgenic plants confirmed that the T-DNA had been integrated into the rice genome. Insect bioassays using T1 transgenic plants indicated that the mortality of the leaffolder (Cnaphalocrasis medinalis) after 7d of leaf feeding reached 38%-61% and the corrected mortality of the striped stem borer (Chilo suppressalis) after 7d of leaf feeding reached 16%-75%. The insect bioassay results demonstrated that the transgenic plants expressing the spider insecticidal protein conferred enhanced resistance to these pests.

谚语“严霜偏杀枯根草”探析

谚语是一种独特的语言形式,以言简意赅而通俗易懂的语言反映人们的生活和认知,它不仅是汉语研究的重要内容,也是历史文化传承的载体。“严霜偏杀枯根草”这一谚语出自元代孟汉卿《张鼎智勘魔合罗》(元刻本),指灾难偏偏降临到处境困难的人或条件差的事物上。随着该谚语在古今不同时代的具体应用,其结构形式也发生了相应的演变,主要表现在词替换和结构繁化两方面。该谚语在发展演变中承载着丰富的文化内涵,不仅反映具象思维、体现福祸观,而且蕴含着联系观。

Study on the Activity of Herbal Extract to Kill Root-knot Nematode of Cucumber Seedlings Stage

Present mature plants hydroponic technology was used,combined with some excellent characteristics,such as growth conditions was easy to control and process of root growth was easy to continuously observe,the nematicidal activity of 5 kinds of Chinese herbs extracts and the compound solution of Avermectin,with strong contact toxicity effect indoor,was systematically studied and investigated the affection on the root-knot nematode parasitized on the cucumber seeding stage. It is found that under the premise of no influence on root growth of cucumber,extracts from Picrorhiza scrophulariiflora and Punica granatum showed strong prevention and nematicidal activity,and had the similar efficacy of Avermectin; while the extracts from Cibotium barometz,Aucklandia lappa Decne and Fructus cnidii showed low nematicidal activity and various degrees inhibition effect on plant growth.

Integrated Control of Meloidogyne Incognita Infesting Tomato Plants Using Callus Powders Derived from Certain Plants Either Alone or in Combination with Oxamyl under Out-Door Conditions

The effect of four dried callus powders derived from certain plants, i.e. tomato, Lycopersicon esculentum; periwinkle, Catharanthus roseus; thorne apple, Datura stramonium and oleander, Nerium oleander applied separately or concomitantly with oxamyl at a half of the recommended dose each on tomato seedlings grown in soil naturally heavy infested with M. incognita under field conditions, indicated that dried callus powder derived from N. oleander either alone or mixed with oxamyl significantly surpassed the other tested treatments in improving percentages of increase in whole plant fresh and shoot dry weights as well as increasing numbers and weights of fruits. Concerning other treatments including oxamyl at a half of recommended dose with each tested callus powder, similar results were evident regarding tomato plant growth parameters. C. roseus plus oxamyl achieved the highest percentage of increase in whole plant fresh weight （141.4%） and shoot dry weight （189.7%） and ranked second to N. oleander ＋ oxamyl in this respect over the untreated plants, respectively. One the other hand, among all tested materials single application of either L. esculentum or oxamyl gave the least values of such criteria which averaged 34.4% and 61.7% or 39.5% and 11.2%, respectively. All tested treatments significantly reduced the total number of galls, egg-masses and eggs in egg-masses especially, dried callus powder of N. oleander which applied singly or combined with oxamyl. The least values of reduction percentages of galls and eggs in egg-masses among all treatments were recorded from tomato plant received dried callus powders of L. esculentum applied alone or concomitant with oxamyl.