For exploring the influences of application and residue of chemical fungi- cides on chlamydospore preparations of Trichoderma spp., the effects of seven chemical fungicides on chlamydospore germination and mycelia gro...For exploring the influences of application and residue of chemical fungi- cides on chlamydospore preparations of Trichoderma spp., the effects of seven chemical fungicides on chlamydospore germination and mycelia growth of two bio- control fungi T. harzianum 610 and T. Iongibrachiatum 758 were studied. Carben- dazim, tebuconazole and difenoconazole showed strong toxicities, thiram and car- bexin showed moderate toxicities, and metalaxyl showed Mycelia growth of the two strains was more sensitive to most tested fungicides than those of chlamydospore germination. Chlamydospore germination of 610 was more sensitive to tested fungicides than those of 758, and mycelia growth of 758 was more sensitive to most tested fungi- cides than those of 610. Among the seven fungicides, 98% carbendazim had the strongest effects (ECru values were 1.64 and 0.05μpg/ml), and 70% pentachloroni- trobenzene had the weakest effects (EC50 values were 1.64 and 0,05 μg/ml) for chlamydospore germination and mycelia growth of 610. As for 758, 98% carbendaz- im had the strongest inhibitory effects and 95% metalaxyl had the weakest inhibitory for chlamydospore germination of 756 (EC50 values were 0.62 and 1 108.61 μg/ml respectively), whereas 96.2% tebuconazole showed the strongest inhibitory effects for mycelia growth of 758 (EC= value was 0.32μg/ml), and 95% metalaxyl was the weakest (EC= value was 1 206.29 μg/ml). According to the applied concentration of different fungicides in practice, we concluded that chlamydospore preparations of 610 and 758 could not be combined with carbendazim, tebuconazole, thiram and carboxin for controlling plant diseases, and the pesticide residues to the biocontrol effects should be kept in mind. Chlamydospore preparations of 610 and 758 can be and difenoconazole for controlling plant dis- eases, 758 chlamydospore preparations and germinated chlamydospore of 610 can be combined with metalaxyl for controlling plant diseases, and pesticide residue risk was not serious.展开更多
The phosphonate fertilizers, Calphos (calcium phosphonate), Magphos (magnesium phosphouate) and Phosphoros (potassium phosphonate) were used in Jordan and showed a nematicidal effect. This study aimed to investi...The phosphonate fertilizers, Calphos (calcium phosphonate), Magphos (magnesium phosphouate) and Phosphoros (potassium phosphonate) were used in Jordan and showed a nematicidal effect. This study aimed to investigate the fungicidal effect of these fertilizers against the growth of the fungi, Fusarium proliferatum, Altrenaria solani, Pythium debaryanum and Penicillium digitatum. Results showed that the phosphonate fertilizer Calphos at the concentration of 1% mixed with potato dextrose agar (PDA) totally inhibited the growth of all studied fungi. Magphos (1%) delayed the growth of the fungus F. proliferatum and inhibited the growth of other tested fungi. While Phosphoros (1%) mixed with PDA inhibited the growth ofA. solani and P. digitatum even after 12 d of incubation and delayed the growth ofF. proliferatum and P. debaryanum compared with the growth of the fungi on the PDA only.展开更多
"Avocado sadness" caused by the Oomycete Phytophthora cinnamomi Rands, is one of the main diseases affecting this fruit crop in Michoacan, M6xico, without having an efficient control strategy for that. In this resea..."Avocado sadness" caused by the Oomycete Phytophthora cinnamomi Rands, is one of the main diseases affecting this fruit crop in Michoacan, M6xico, without having an efficient control strategy for that. In this research three Trichoderma isolates and Ridomil Gold 4E fungicide were tested in avocado trees infected with this pathogen using the growing and coloring vegetative flow growing. Results were analyzed with an experimental design completely random with four treatments and repetitions. Different responses were presented form avocado trees to each treatment. Trichoderma erinaceum Bissett, Kubicek & Szackacs treatment was the best controlling disease in trees with grade II and III damage. Trichoderma aggressivum Samuels & W. Gams controlled better in trees with grade III damage. At last, Trichoderma arundinaceum Zafari, Graef. and Samuels were the best controlling trees with the highest grade damage. Fungicide Ridomil Gold 4E controlled the disease in trees with the lowest grade damage (I and II), their medias were similar to witness.展开更多
The objective of this study was to test the essential oil-emitted flavor (volatile) of lavender by bacteria killing potency using Escherichia coli, methicillin-resistant Staphylococcus aureus (MRSA), enterohemorrh...The objective of this study was to test the essential oil-emitted flavor (volatile) of lavender by bacteria killing potency using Escherichia coli, methicillin-resistant Staphylococcus aureus (MRSA), enterohemorrhagic E. coli O157:H7, Pseudomonas aeruginosa and Candida albicans. Antibacterial activity was assessed by creating of the bacterial growth curve in the liquid medium cultivation and the growth inhibition on the agar plate cultivation. Citronellal, one of compounds in Rosa rugose oil, was used as a positive control for comparison in activity. The results showed that lavender and citronellal flavor (volatile) or oil respectively demonstrated bacteria killing effect in both analytical manners. However, P. aeruginosa resisted bacteria killing potency of lavender (citronellal) oil or oil-emitted flavor. It could be concluded that both lavender oil-emitted flavor and oil showed bacteria killing potency. Lavender oil (or oil-emitted flavor) will be expected to apply for the patient waiting room to disinfect in air along with the mental relaxation of the patient.展开更多
基金Supported by Special Fund for Agro-scientific Research in the Public Interest(201303057)948 Program(2011-G4)~~
文摘For exploring the influences of application and residue of chemical fungi- cides on chlamydospore preparations of Trichoderma spp., the effects of seven chemical fungicides on chlamydospore germination and mycelia growth of two bio- control fungi T. harzianum 610 and T. Iongibrachiatum 758 were studied. Carben- dazim, tebuconazole and difenoconazole showed strong toxicities, thiram and car- bexin showed moderate toxicities, and metalaxyl showed Mycelia growth of the two strains was more sensitive to most tested fungicides than those of chlamydospore germination. Chlamydospore germination of 610 was more sensitive to tested fungicides than those of 758, and mycelia growth of 758 was more sensitive to most tested fungi- cides than those of 610. Among the seven fungicides, 98% carbendazim had the strongest effects (ECru values were 1.64 and 0.05μpg/ml), and 70% pentachloroni- trobenzene had the weakest effects (EC50 values were 1.64 and 0,05 μg/ml) for chlamydospore germination and mycelia growth of 610. As for 758, 98% carbendaz- im had the strongest inhibitory effects and 95% metalaxyl had the weakest inhibitory for chlamydospore germination of 756 (EC50 values were 0.62 and 1 108.61 μg/ml respectively), whereas 96.2% tebuconazole showed the strongest inhibitory effects for mycelia growth of 758 (EC= value was 0.32μg/ml), and 95% metalaxyl was the weakest (EC= value was 1 206.29 μg/ml). According to the applied concentration of different fungicides in practice, we concluded that chlamydospore preparations of 610 and 758 could not be combined with carbendazim, tebuconazole, thiram and carboxin for controlling plant diseases, and the pesticide residues to the biocontrol effects should be kept in mind. Chlamydospore preparations of 610 and 758 can be and difenoconazole for controlling plant dis- eases, 758 chlamydospore preparations and germinated chlamydospore of 610 can be combined with metalaxyl for controlling plant diseases, and pesticide residue risk was not serious.
文摘The phosphonate fertilizers, Calphos (calcium phosphonate), Magphos (magnesium phosphouate) and Phosphoros (potassium phosphonate) were used in Jordan and showed a nematicidal effect. This study aimed to investigate the fungicidal effect of these fertilizers against the growth of the fungi, Fusarium proliferatum, Altrenaria solani, Pythium debaryanum and Penicillium digitatum. Results showed that the phosphonate fertilizer Calphos at the concentration of 1% mixed with potato dextrose agar (PDA) totally inhibited the growth of all studied fungi. Magphos (1%) delayed the growth of the fungus F. proliferatum and inhibited the growth of other tested fungi. While Phosphoros (1%) mixed with PDA inhibited the growth ofA. solani and P. digitatum even after 12 d of incubation and delayed the growth ofF. proliferatum and P. debaryanum compared with the growth of the fungi on the PDA only.
文摘"Avocado sadness" caused by the Oomycete Phytophthora cinnamomi Rands, is one of the main diseases affecting this fruit crop in Michoacan, M6xico, without having an efficient control strategy for that. In this research three Trichoderma isolates and Ridomil Gold 4E fungicide were tested in avocado trees infected with this pathogen using the growing and coloring vegetative flow growing. Results were analyzed with an experimental design completely random with four treatments and repetitions. Different responses were presented form avocado trees to each treatment. Trichoderma erinaceum Bissett, Kubicek & Szackacs treatment was the best controlling disease in trees with grade II and III damage. Trichoderma aggressivum Samuels & W. Gams controlled better in trees with grade III damage. At last, Trichoderma arundinaceum Zafari, Graef. and Samuels were the best controlling trees with the highest grade damage. Fungicide Ridomil Gold 4E controlled the disease in trees with the lowest grade damage (I and II), their medias were similar to witness.
文摘The objective of this study was to test the essential oil-emitted flavor (volatile) of lavender by bacteria killing potency using Escherichia coli, methicillin-resistant Staphylococcus aureus (MRSA), enterohemorrhagic E. coli O157:H7, Pseudomonas aeruginosa and Candida albicans. Antibacterial activity was assessed by creating of the bacterial growth curve in the liquid medium cultivation and the growth inhibition on the agar plate cultivation. Citronellal, one of compounds in Rosa rugose oil, was used as a positive control for comparison in activity. The results showed that lavender and citronellal flavor (volatile) or oil respectively demonstrated bacteria killing effect in both analytical manners. However, P. aeruginosa resisted bacteria killing potency of lavender (citronellal) oil or oil-emitted flavor. It could be concluded that both lavender oil-emitted flavor and oil showed bacteria killing potency. Lavender oil (or oil-emitted flavor) will be expected to apply for the patient waiting room to disinfect in air along with the mental relaxation of the patient.