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植物病原真菌对杀菌剂抗性的研究进展 被引量:6
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作者 吴小美 王海霞 +1 位作者 云英子 马忠华 《植物保护》 CAS CSCD 北大核心 2023年第5期243-259,共17页
科学施用杀菌剂是植物病害综合治理的重要措施之一,然而由于杀菌剂的长期使用,病菌抗药性问题逐渐加重,严重影响药剂的防治效果和使用寿命。近年来,随着分子生物学技术的快速发展,人们对杀菌剂抗性机制有了更深入的理解,并开发出了病菌... 科学施用杀菌剂是植物病害综合治理的重要措施之一,然而由于杀菌剂的长期使用,病菌抗药性问题逐渐加重,严重影响药剂的防治效果和使用寿命。近年来,随着分子生物学技术的快速发展,人们对杀菌剂抗性机制有了更深入的理解,并开发出了病菌抗药基因型快速检测的方法。本文总结了植物病原真菌对苯并咪唑类杀菌剂(BZD)、肌球蛋白合成抑制剂、甾醇脱甲基抑制剂(DMI)、QoI类抑制剂、琥珀酸脱氢酶抑制剂(SDHI)和二甲酰亚胺类杀菌剂(DC)的抗药性现状与抗性机制。在此基础上,介绍了聚合酶链反应(PCR)、限制性片段长度多态性(RFLP)、等位基因特异性PCR和环介导等温扩增(LAMP)技术在杀菌剂抗性快速检测方面的研究进展。此外,对抗药性治理对策进行了讨论和展望。 展开更多
关键词 植物病原真菌 杀菌剂抗性机制 抗性检测 抗性治理对策
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Effect of Different Bioassay Methods on Enzymatic Characteristics of Cotton Aphid, Aphis gossypfi Glover (Hemiptera:Aphididae)
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作者 Shadieh Gerami Ahmad Heidari 《Journal of Agricultural Science and Technology(A)》 2013年第10期819-824,共6页
In this study, the susceptibility of three populations of cotton aphid, Aph& gossypii Glover (Hemiptera:Aphididae) was assayed to imidacloprid (35SC) and thiametoxam (50WG). The involvement of metabolic enzyme... In this study, the susceptibility of three populations of cotton aphid, Aph& gossypii Glover (Hemiptera:Aphididae) was assayed to imidacloprid (35SC) and thiametoxam (50WG). The involvement of metabolic enzymes in the resistance strain of cotton aphid to the neonicotinoids was determined by the biochemical biomarkers and the resistance mechanism was determined as CaE. In another study, three different bioassay experiments were designed for detecting the susceptibility of cotton aphid to imidacloprid and thiametoxam and the effect of these two insecticides on the enzymatic activity of cotton aphid was assessed in the adult aphids treated with three different bioassay methods using a modified version of the FAO dip test, residue bioassay procedure and starvation method. Our findings suggested that the type of bioassay methods is very important when aphids' populations assess for the resistance against the neonicotinod insecticides. It has shown the starvation method is the most reliable method compared with other methods. 展开更多
关键词 IMIDACLOPRID thiametoxam enzymatic activity biochemical biomarker CaE.
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