[Objective] This study aimed to screen a set of SSR core primers suitable for purity identification of pepper (Capsicum) hybrids. [Method] DNA fingerprint of 100 pepper hybrids was analyzed using 17 SSR primers. [Re...[Objective] This study aimed to screen a set of SSR core primers suitable for purity identification of pepper (Capsicum) hybrids. [Method] DNA fingerprint of 100 pepper hybrids was analyzed using 17 SSR primers. [Result] According to the polymorphism and heterozygosity, Hpms1-214, Es395 and Hpmsl-5 were determined as three preferred core primers for purity identification of pepper hybrids. By using these three preferred core primers, 97 pepper hybrids (accounting for 97%) had heterozygous band pattern with at least one primer. Es330, Es363, Epms923, Es120 and Es64 were determined as candidate core primers for purity identification of pepper hybrids. Specific primers of 14 varieties were obtained, which could be used to further screen parent-complementary primers of each pepper hybrid. [Con- clusion] This study laid the foundation for constructing standard DNA fingerprints for purity identification of pepper hybrids.展开更多
Different results of seed purity identification for Gangyou 158, Ⅱ You 808, Wuyou 308 and Tianfengyou 316 were obtained using different SSR primers in our early work. To find out the reasons, the four hybrid combinat...Different results of seed purity identification for Gangyou 158, Ⅱ You 808, Wuyou 308 and Tianfengyou 316 were obtained using different SSR primers in our early work. To find out the reasons, the four hybrid combinations were grown in field to identify their purity according to their phenotypic traits. Then, the results of field identification were compared with that of laboratory tests using different SSR primers. The comparison revealed that only sterile lines (female parent) were distin- guished from true hybrids using the primers RM208, RM264, RM242 and RM164 for the purity identification of Gangyou 158, II You 808, Wuyou 308 and Tianfengyou 316, so the results were higher than that of field identification. In contrast, the primers RM341, RM297, RM21 and RM110 were able to distinguish not only the sterile plants but also the cross-pollinated ones from the true hybrids of Gangyou 158,Ⅱ You 808, Wuyou 308 and Tianfengyou 316, and the results of purity identifi- cation using them were close that of field identification, in summary, several pairs of primers should be used for the purity identification of rice hybrids to distinguish all the off-type plants and thus improve the accuracy.展开更多
基金Supported by Excellent Team Training Program of Yunnan Academy of Agriculture Sciences(YAAS2014YY002)~~
文摘[Objective] This study aimed to screen a set of SSR core primers suitable for purity identification of pepper (Capsicum) hybrids. [Method] DNA fingerprint of 100 pepper hybrids was analyzed using 17 SSR primers. [Result] According to the polymorphism and heterozygosity, Hpms1-214, Es395 and Hpmsl-5 were determined as three preferred core primers for purity identification of pepper hybrids. By using these three preferred core primers, 97 pepper hybrids (accounting for 97%) had heterozygous band pattern with at least one primer. Es330, Es363, Epms923, Es120 and Es64 were determined as candidate core primers for purity identification of pepper hybrids. Specific primers of 14 varieties were obtained, which could be used to further screen parent-complementary primers of each pepper hybrid. [Con- clusion] This study laid the foundation for constructing standard DNA fingerprints for purity identification of pepper hybrids.
基金Supported by Special Fund for the Screening and Breeding of Low-Cd-accumulating Crop Varieties~~
文摘Different results of seed purity identification for Gangyou 158, Ⅱ You 808, Wuyou 308 and Tianfengyou 316 were obtained using different SSR primers in our early work. To find out the reasons, the four hybrid combinations were grown in field to identify their purity according to their phenotypic traits. Then, the results of field identification were compared with that of laboratory tests using different SSR primers. The comparison revealed that only sterile lines (female parent) were distin- guished from true hybrids using the primers RM208, RM264, RM242 and RM164 for the purity identification of Gangyou 158, II You 808, Wuyou 308 and Tianfengyou 316, so the results were higher than that of field identification. In contrast, the primers RM341, RM297, RM21 and RM110 were able to distinguish not only the sterile plants but also the cross-pollinated ones from the true hybrids of Gangyou 158,Ⅱ You 808, Wuyou 308 and Tianfengyou 316, and the results of purity identifi- cation using them were close that of field identification, in summary, several pairs of primers should be used for the purity identification of rice hybrids to distinguish all the off-type plants and thus improve the accuracy.