用SARS冠状病毒全基因组芯片杂交方法分析SARS-CoV

为从临床样品中检测和分析SARS CoV病毒打基础 ,并为分析SARS CoV病毒的复制和转录等机理提供一种有效方法。以SARS冠状病毒TOR2株序列作为标准设计和制备一种覆盖SARS冠状病毒全基因组的寡聚核苷酸芯片 ,探针长度为 70nt,每相邻的探针序列重复 2 5nt,共660条。用该芯片分析了细胞培养的SARS CoV病毒总RNA、7个SARS CoV病毒的基因克隆片段。对RNA样品用随机引物进行反转录PCR获得cDNA。对DNA用随机引物扩增和dUTP cy3标记。结果用这种芯片杂交检测SARS CoV病毒RNA可见阳性信号呈全基因组分布 ,并且有多处连续的阳性信号点 ;用正常人的白细胞RNA为对照 ,杂交未出现明显阳性信号。检测 7个SARS CoV病毒基因克隆片段 ,在该片段相应的探针区段出现连续阳性信号点。这种方法可有效地检测和分析样品中SARS冠状病毒全基因组的信息。

人兽杂交splice

克莱夫和艾丽莎是基因工程界的天王级学者，他们收揽了无数科学界的荣誉，具有无上的光环。在他们的研究领域内，两人更擅长于基因融合和基因杂交方面的研究，并发现很多情况下，由基因杂交而诞生的新物种具有更高的智慧和更强的身体机能。

Expression Profiles of TRAIL Receptors and Their Clinical Significance in Human Hepatocellular Carcinoma

Objective To investigate the expression profiles and their clinical significance of TRAIL receptors (TRAILR) in human hepatocellular carcinoma (HCC). Methods The expression profiles of TRAILR were determined in 60 samples from hepatocellular carcinoma, 20 from normal liver tissue and two HCC cell lines HepG2, SMMC-7721 by in situ hybridization. Results Both DR4 and DR5 were present in all HCC tissues as well as normal hepatic tissues. In contrast, 54 HCC tissues did not express DcR1 and 25 did not express DcR2. But both DcR were detectable in all of the normal liver tissues. The expression patterns of DR and DcR in HCC samples (higher DR expression level and lower DcR expression level) were quite different from those in normal tissue. DR5, DR4, and DcR2 expressed in both cell lines, while no DcR1 expression was detected. The expression level of DR was correlated with HCC differentiation and stage. The weaker expression was more commonly found in HCC with poor differentiation and late stage, while the stronger expression was more common in HCC with middle to high-differentiation and early stage. No relationship was found between DR and gender, age, negative or positive HBsAg, tumor size, grade or metastasis. Multidrug resistance cell lines expressed lower level DR. Conclusion TRAILR expression was prevalent and discrepancy of receptor types was exited in HCC. Loss of DcR1 may contribute for TRAIL therapy for HCC. Key words TRAILR - apoptosis - hepatocellular carcinoma Supported by the Major Fundation of Ministry of Health, NO. 2001–2003

Effects of Cultivation Methods on Yield Formation and Photosynthetic Characteristics of Zhuliangyou 4024 and H You 159 as Doublecropping Rice

To explore the high-yield cultivation methods of Zhuliangyou 4024 and H You 159 when planted as double-cropping rice, the effects of two cultivation meth- ods, i.e., seedling broadcasting and transplanting, were studied on SPAD value, photosynthetic rate, grain yield and yield components. The results showed that the seedling broadcasting cultivation was conducive to Zhuliangyou 4024 and H You 159 to obtain higher yield. The higher chlorophyll content and photosynthetic rate and their slower decrease of flag leaf after full heading of the seedling broadcasting cultivation were thought to be the physiological basis of high yield.

An intersection method for locating earthquakes in complex velocity models

The intersection method is one of the basic approaches for locating earthquakes and is not only robust but also efficient. However, its location accuracy is not high, especially for focal depth because the velocity model used for the conventional intersection method is based on homogeneous or laterally homogeneous media, which is too simple. In order to improve the accuracy, we have modified the existing intersection method. In the modified approach, the earthquake loci are not assumed to be circular or hyperbolic and calculation accuracy is improved using a minimum traveltime tree algorithm for tracing rays. The numerical model shows that the modified method can locate earthquakes in complex velocity models.

Growth Performance of Hybrid Progenies of Grass Carp (Ctenopharyngodon idella) Populations from Different Geographical Origins

[Objective] This study aimed to evaluate growth performance of hybrid progenies of 10 grass carp （Ctenopharyngodon idella） populations from different ge- ographical origins （Foshan population, Zhaoqing population, Jingzhou population 1, Jingzhou population 2, Jingzhou population 3, Ezhou population, Yiyang population, Changsha population 1, Changsha population 2 and Jiangsu population）. [Method] Fifteen hybrid combinations were established with these ten grass carp populations and cultured in the same pond. The body mass of hybrid progenies was measured at the age of 4, 7, 8, 10, 12, 15 and 18 months, respectively. Using body mass at the age of 4 months as a concomitant variable, the growth performance of proge- nies from different hybrid combinations was analyzed by covariance analysis. [Re- suit] The average body mass of hybrid progenies of Jingzhou population 2 c2 x Foshan population $ reached the maximum （1 892.90 g） at the age of 18 months, which was 3.51%-32.36% higher than that of other hybrid progenies. Multiple com- parison analysis showed that average body mass of hybrid progenies of Jingzhou population 2 2 x Foshan population ♂ and Jingzhou population 1 ♀× Foshan population ♂ was significantly higher than that of other hybrid progenies （P〈0.05）. [Conclusion] In the production process, Jingzhou population 2 ♀ × Foshan popula- tion 5 and Jingzhou population 1 ♀×Foshan population ♂ can be used to pro- duce high-quality fries, thereby greatly improving economic benefits. This study laid the foundation for establishment of rapid-growing grass carp core populations.

An Innovative Strategy for Reciprocal Distant Hybridization between Spartina alterniflora and Rice

[Objective] This study aimed to explore germplasm resources of salt toler- ant Spartina alterniflora and high yield rice, and develop an innovative strategy for distant hybridization between S. altemiflora and rice. [Method] Crossing （rice ♀ ）： hot water emasculation and glume-cutting pollination method, heat emasculation and pollen-suction pollination method; reciprocal crossing （S. alterniflora ♀ glume-cutting emasculation and pollination method, continuous emasculation-pollination method, hot water emasculation and continuous emasculation-pollination method. [Result] In crossing groups （rice ♀ × S. alterniflora ♂ ）, compared with hot water emasculation and glume-cutting pollination treatment, the number of seed-bearing combinations, seed number per spike and seedling emergence rate in heat emasculation and pollen-suction pollination treatment were improved by 34.09%, 121.21% and 60.07%, respectively; hybridization efficiency was improved by 6-7 times. In reciprocal cross- ing groups （S. altemiflora ♀× rice ♂ ）, compared with glume-cutting emasculation and pollination treatment, the number of seed-bearing combinations and seed num- ber per spike in continuous emasculation-pollination treatment were improved by 3.14 and 4.21 times, respectively; seedling emergence rate was improved by 68.47%; hybridization efficiency was improved by 7-8 times. [Conclusion] Heat emas- culation and pollen-suction pollination method is suitable for hybridization between rice ♀× S. alterniflora ♂; continuous emasculation-pollination method is suitable for hybridization between S. alterniflora ♀× rice ♂.

Rapid detection of intestinal pathogens in fecal samples by an improved reverse dot blot method

AIM:To develop a new, rapid and accurate reverse dot blot(RDB) method for the detection of intestinal pathogens in fecal samples.METHODS:The 12 intestinal pathogens tested were Salmonella spp., Brucella spp., Escherichia coli O157:H7, Clostridium botulinum , Bacillus cereus , Clostridium perfringens , Vibrio parahaemolyticus , Shigella spp., Yersinia enterocolitica, Vibrio cholerae, Listeria monocytogenes and Staphylococcus aureus.The two universal primers were designed to amplify two variable regions of bacterial 16S and 23S rDNA genes from all of the 12 bacterial species tested.Five hundred and forty fecal samples from the diarrhea patients were detected using the improved RDB assay.RESULTS:The methods could identify the 12 intestinal pathogens specifi cally, and the detection limit was as low as 103 CFUs.The consistent detection rate of the improved RDB assay compared with the traditional culture method was up to 88.75%.CONCLUSION:The hybridization results indicated that the improved RDB assay developed was a reliable method for the detection of intestinal pathogen in fecal samples.

Agronomic Characters of Elymus dahuricus,Hordeum brevisubulatum and Their BC1F3

[ Objective] This study was to explore the inheritance of BC1 F3 to excellent characteristics of parents（ Elymus dahuricus, Hordeum brevisubu/atum）, heterosis and fertility restoration. E Methodl The agronomic characters such as growth period, growth rate, pollen fertility, fecundity, reproducibility and fresh grass output of Elymus dahudcus, Hordeum brevisubu/atum and BC1 F3 were comparatively studied. E Result l The growth dynamic of the BC1 F3 lined tended to like that of its parent Hordeum brevisubu/atum; although growing periods of various lines were different, they were close to that of Hordeurn brevisubulatum. There were differences in pollen fertility and seed setting, the pollen fertility rate of YF3-93 was higher than recurrent parent Hordeum brevisubulatum, its seed setting rate in open pollination was also higher. Some lines had low pollen fertility rate and seed setting rate, such as the pollen fertility rate of PF3-52. There were significant differences in the output among BC1 F3 lines, e.g. the heterosis （HP） of YF3-64, YF3-74 and YF3-83 were 75.53%, 75.12% and 66.16%, respectively; however, the yields of PF3- 52, PF3-15 and PF3-42 were lower than their parents. ~ Conclusion] This study provided reference for breeding new varieties of forage which is suitable to drought and saline environment.

A novel adjustable multiple cross-hexagonal search algorithm for fast block motion estimation

In this paper,we propose a novel adjustable multiple cross-hexagonal search(AMCHS) algorithm for fast block motion estimation. It employs adjustable multiple cross search patterns(AMCSP) in the first step and then uses half-way-skip and half-way-stop technique to determine whether to employ two hexagonal search patterns(HSPs) subsequently. The AMCSP can be used to find small motion vectors efficiently while the HSPs can be used to find large ones accurately to ensure prediction quality. Simulation results showed that our proposed AMCHS achieves faster search speed,and provides better distortion performance than other popular fast search algorithms,such as CDS and CDHS.

Effects of main factors on remedying northern landscaping water by hybrid ecological filter

Natural zeolite and coal cinder were layered as main packing medium of the ecological filter instead of traditional filling to remedy the static lake water in Northern China.The ecological filter was running in a combined upward and downward flow mode.Dynamic experiments were carried out to study the effects of retention time and ambient temperature on pollutants' removal efficiency of the hybrid ecological filter.The function of plant was also studied by contrast test.It is showed that the removal efficiencies of NH4+-N and TP are increased when the retention time is changed from 1 h to 2 h and 4 h,but the removal efficiency of TN is decreased,the removal efficiency of NH4+-N is increased from 91.5% to 98% and that of TP is increased from 31.8% to 52.5%.When the temperature declines,the temporal removal efficiency of NH4+-N is reduced,but the removal efficiency of 24 h and 48 h is remained.The removal efficiency of TP after 24 h and 48 h is decreased when the temperature declines evidently.The retention time plays an important role in NH4+-N and TP removal,and the ambient temperature is significant for TP removal.The plant favors for TP and organic matter removal but has little effect on TN removal.

Singular Hybrid Boundary Node Method for Solving Poisson Equation

As a boundary-type meshless method, the singular hybrid boundary node method（SHBNM） is based on the modified variational principle and the moving least square（MLS） approximation, so it has the advantages of both boundary element method（BEM） and meshless method. In this paper, the dual reciprocity method（DRM） is combined with SHBNM to solve Poisson equation in which the solution is divided into particular solution and general solution. The general solution is achieved by means of SHBNM, and the particular solution is approximated by using the radial basis function（RBF）. Only randomly distributed nodes on the bounding surface of the domain are required and it doesn＇t need extra equations to compute internal parameters in the domain. The postprocess is very simple. Numerical examples for the solution of Poisson equation show that high convergence rates and high accuracy with a small node number are achievable.

Genetic Variations and Gene Effect Controlling Grain Yield and Some of Its Components in Maize

Diallel cross analysis among seven diverse pure lines of maize, （IK8, IK58, ZP-707, OH40, UN44052, DKI7 and HS） with their nonreciprocal 21 crosses, were carried out for estimating the nature and magnitude of gene effects through component and graphic analysis for characters： number of days to tasseling, number of days to silking, plant height, ear height, number of rows per ear, number of grains per row, 300 grain weight and grain yield per plant. The results showed highly significant mean square of genotypes for all studied characters. Additive and dominance variances were found to be significant from zero for all the eight characters except number of days to tasseling and silking for which additive genetic variance was no significant. Dominance variance estimation was relatively higher than the additive variance for all characters. The graphic analysis suggested the presence of epistatic gene effects （non-allelic interaction） for number of days to tasseling, number of days to silking, plant height, number of grains per row and grain yield per plant. The estimates of narrow sense heritability varied from 1.85% for 300 grain weight to 49.88% for ear height, it was moderate in case of ear height and low for the remainder characters.

Magnetic particles and cadmium sulfide nanoparticles tagging for signal-amplifying detection of nucleic acids

A versatile DNA recognition system using cadmium sulfide nanoparticles (CdS NPs) as labels was proposed for ultrasensitive detection of specific sequence DNA based on target recycling. This strategy utilized the magnetic particles (MNPs) for the immobilization of linker DNA and CdS NPs and the subsequent target DNA hybridization. Using the unique characteristic of nicking endonuclease for cutting one specific strand of double strand DNA (ds DNA), the linker DNA could be transected and the target DNA could be liberated for re-hybridization and hence the amount of released CdS NPs was enhanced. Due to the advantage of the MNPs and signal amplification from the target recycling, the analyte DNA could be detected by the square-wave stripping voltammetry (SWV) in a wide linear range from 0.4 fM to 100 fM with the detection limit down to 0.08 fM. The proposed DNA detection strategy possesses high sensitivity, satisfactory reproducibility and excellent stability, which might have potential in other DNA biological assays.

A hybridized weak Galerkin finite element scheme for the Stokes equations

In this paper a hybridized weak Galerkin(HWG) finite element method for solving the Stokes equations in the primary velocity-pressure formulation is introduced.The WG method uses weak functions and their weak derivatives which are defined as distributions.Weak functions and weak derivatives can be approximated by piecewise polynomials with various degrees.Different combination of polynomial spaces leads to different WG finite element methods,which makes WG methods highly flexible and efficient in practical computation.A Lagrange multiplier is introduced to provide a numerical approximation for certain derivatives of the exact solution.With this new feature,the HWG method can be used to deal with jumps of the functions and their flux easily.Optimal order error estimates are established for the corresponding HWG finite element approximations for both primal variables and the Lagrange multiplier.A Schur complement formulation of the HWG method is derived for implementation purpose.The validity of the theoretical results is demonstrated in numerical tests.