[ Objective] This study was to investigate the distribution of H-FABP mRNA in hybrids of Songliao black pig x Sus scrofa, so as to provide references for revealing the physiological functions of this gene. [Method] Wi...[ Objective] This study was to investigate the distribution of H-FABP mRNA in hybrids of Songliao black pig x Sus scrofa, so as to provide references for revealing the physiological functions of this gene. [Method] With the mRNA from different tissues as template, RT-PCR amplification was carried out for cloning cDNA of hybrid pig, which was then used for PCR reaction using specific primers; the amplification products were separated by gel electrophoresis and analyzed by sequencing for detecting the distribution pattern of H-FABP mRNA in hybrids of Songliao black pig x Sus scrofa. [ Result] H-FABP expressed in all the twelve tissues including subcutaneous fat, abdominal fat, mammary gland, Iongissimus dorsi muscle, dorsal deltoid muscle, heart, liver, spleen, lung, kidney, ileum and duodenum. [ Conclusion] The wide distribution of H-FABP gene suggests that its functions are important and multiple.展开更多
文摘试验旨在研究杂交野猪猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)辽宁分离株的遗传变异情况及分子生物学特征。用Marc-145细胞从辽宁某杂交野猪场疑似猪繁殖与呼吸综合征(porcine reproductive and respiratory syndrome,PRRS)病猪血液中分离到1株病毒,该分离毒株经Marc-145细胞6次传代后出现稳定的细胞病变,采用RT-PCR方法对分离病毒进行ORF6和ORF7基因的扩增、克隆和测序,并与已知序列毒株的相应片段进行同源性比对。结果表明,分离毒株的ORF6、ORF7基因与国内外美洲型毒株的核苷酸同源性分别为96.0%~100.0%、94.5%~99.4%;氨基酸同源性分别为89.6%~100.0%、87.3%~98.7%;与欧洲型代表毒株LV的ORF6、ORF7基因差异较大,核苷酸同源性分别为70.4%、70.1%,氨基酸同源性分别为48.8%、49.7%。推测辽宁杂交野猪体内分离毒株在基因型上属于美洲型毒株。
基金Supported by the National High Technology Research and Develop-ment Program of China(No.2008AA10Z136)the Program of Liaoning Provincial Science-Technology Department(20070567)~~
文摘[ Objective] This study was to investigate the distribution of H-FABP mRNA in hybrids of Songliao black pig x Sus scrofa, so as to provide references for revealing the physiological functions of this gene. [Method] With the mRNA from different tissues as template, RT-PCR amplification was carried out for cloning cDNA of hybrid pig, which was then used for PCR reaction using specific primers; the amplification products were separated by gel electrophoresis and analyzed by sequencing for detecting the distribution pattern of H-FABP mRNA in hybrids of Songliao black pig x Sus scrofa. [ Result] H-FABP expressed in all the twelve tissues including subcutaneous fat, abdominal fat, mammary gland, Iongissimus dorsi muscle, dorsal deltoid muscle, heart, liver, spleen, lung, kidney, ileum and duodenum. [ Conclusion] The wide distribution of H-FABP gene suggests that its functions are important and multiple.
