An intergeneric artificial hybridization was conducted between Cunninghamia R. Br. and Cryptomeria D.Don The results are as follows:1. A considerable number of hybrid seeds shed from 76 pollinated cones were ...An intergeneric artificial hybridization was conducted between Cunninghamia R. Br. and Cryptomeria D.Don The results are as follows:1. A considerable number of hybrid seeds shed from 76 pollinated cones were empty and a total of 628 looks plump. Soft X ray radiographs showed that, still and all, a majority of the “plump" seeds were embryoless (597, 95.6%) whereas some were partially developed (17,2.7%) and only a few were really full (14, 2.2%). 2. Germination test showed that all of the radiographed hybrid seeds with fully developed embryos were germinable whereas those with partially developed embryos were ungerminable. 3. Physiologically, the growth rate of hypocotyl, the date for shedding of seed coat and spreading of cotyledons, the elongation of epicotyl, and the branching of shoot of the 11 month old seedlings showed a tendency to fall behind those of the female parent; morphologically, the 11 month old hybrid seedlings with linear leaves appeared rather short, slender and weak, whereas the seedlings of the female parents with linear_lanceolate leaves appeared rather tall, stout and strong. 4. It is considered that the hybrid may be true and the crossability reveals a close phylogenetic affinity of Cunninghamia with Cryptomeria.展开更多
A direct interpolation algorithm for spatial curves on a surface is proposed for pen-cutting of sculptured surfaces. The algorithm can carry out the direct interpolation for projective curves lying on the sculptured s...A direct interpolation algorithm for spatial curves on a surface is proposed for pen-cutting of sculptured surfaces. The algorithm can carry out the direct interpolation for projective curves lying on the sculptured surface. It is based on the geometric and kinetic relationships between drive curves and cutter-contact （C-C） curves. It evaluates the parameter of drive curves corresponding to interpolation points by the Taylor formula. Then it gains coordinates of interpolation points indirectly by inverse calculation. Finally, it generates the motion commands for machine tool controller. This method extends the locus-controlled function in the computer numerical control （CNC） system effectively and improves the efficiency for the numerical control （NC） machining of sculptured surfaces. The simulation shows that the proposed algorithm is feasible and practical. This algorithm can also be applied to the machining of the whole surface.展开更多
NC machining path of sculptured surfaces in CAD/CAM system plays an important role on manufacture. This paper describes a new algorithm for 5 axis machining of sculptured surfaces and the algorithm is interference fr...NC machining path of sculptured surfaces in CAD/CAM system plays an important role on manufacture. This paper describes a new algorithm for 5 axis machining of sculptured surfaces and the algorithm is interference free. The approach includes: (1) the tesselation of the parametric surfaces into triangles; (2) building topological relations among triangles;(3) 5 axis tool path generation; (4) interference detection and tool position correction.展开更多
[ Objective] The aim of this study was to provide a method for solving the problems in preparing BAC vector with High-copy plasmid pUC119- Bluelox BAC. [ Method ] With selecting a proper single restriction site, seque...[ Objective] The aim of this study was to provide a method for solving the problems in preparing BAC vector with High-copy plasmid pUC119- Bluelox BAC. [ Method ] With selecting a proper single restriction site, sequences of a single copy BAC vector plasmid were inserted into proper site of High-copy plasmid pUC119 vector. [ Result] The gene sequence of BAC vector lost control function of single copy number in new plasmid pUC119-BAC and was copied through High-copy form. The gene sequence of BAC vector basic function was completely cutted off through single enzyme digestion and the control function of single copy could be recovered by auto-connection. [ Conclusion] The High-copy pUC119-BAC plasmid was used to copy and amplify high copy of basic function gene sequence in BAC vector, besides that it could be used to construct transfer vector of molecular cloned recombinant virus or BAC library.展开更多
文摘An intergeneric artificial hybridization was conducted between Cunninghamia R. Br. and Cryptomeria D.Don The results are as follows:1. A considerable number of hybrid seeds shed from 76 pollinated cones were empty and a total of 628 looks plump. Soft X ray radiographs showed that, still and all, a majority of the “plump" seeds were embryoless (597, 95.6%) whereas some were partially developed (17,2.7%) and only a few were really full (14, 2.2%). 2. Germination test showed that all of the radiographed hybrid seeds with fully developed embryos were germinable whereas those with partially developed embryos were ungerminable. 3. Physiologically, the growth rate of hypocotyl, the date for shedding of seed coat and spreading of cotyledons, the elongation of epicotyl, and the branching of shoot of the 11 month old seedlings showed a tendency to fall behind those of the female parent; morphologically, the 11 month old hybrid seedlings with linear leaves appeared rather short, slender and weak, whereas the seedlings of the female parents with linear_lanceolate leaves appeared rather tall, stout and strong. 4. It is considered that the hybrid may be true and the crossability reveals a close phylogenetic affinity of Cunninghamia with Cryptomeria.
基金The Natural Science Foundation of Jiangsu Province(No.BK2003005).
文摘A direct interpolation algorithm for spatial curves on a surface is proposed for pen-cutting of sculptured surfaces. The algorithm can carry out the direct interpolation for projective curves lying on the sculptured surface. It is based on the geometric and kinetic relationships between drive curves and cutter-contact （C-C） curves. It evaluates the parameter of drive curves corresponding to interpolation points by the Taylor formula. Then it gains coordinates of interpolation points indirectly by inverse calculation. Finally, it generates the motion commands for machine tool controller. This method extends the locus-controlled function in the computer numerical control （CNC） system effectively and improves the efficiency for the numerical control （NC） machining of sculptured surfaces. The simulation shows that the proposed algorithm is feasible and practical. This algorithm can also be applied to the machining of the whole surface.
文摘NC machining path of sculptured surfaces in CAD/CAM system plays an important role on manufacture. This paper describes a new algorithm for 5 axis machining of sculptured surfaces and the algorithm is interference free. The approach includes: (1) the tesselation of the parametric surfaces into triangles; (2) building topological relations among triangles;(3) 5 axis tool path generation; (4) interference detection and tool position correction.
文摘[ Objective] The aim of this study was to provide a method for solving the problems in preparing BAC vector with High-copy plasmid pUC119- Bluelox BAC. [ Method ] With selecting a proper single restriction site, sequences of a single copy BAC vector plasmid were inserted into proper site of High-copy plasmid pUC119 vector. [ Result] The gene sequence of BAC vector lost control function of single copy number in new plasmid pUC119-BAC and was copied through High-copy form. The gene sequence of BAC vector basic function was completely cutted off through single enzyme digestion and the control function of single copy could be recovered by auto-connection. [ Conclusion] The High-copy pUC119-BAC plasmid was used to copy and amplify high copy of basic function gene sequence in BAC vector, besides that it could be used to construct transfer vector of molecular cloned recombinant virus or BAC library.
