In this study,genetic stability of foreign genes was checked by using PCR technology. A total of 4 lines of soil samples from transgenic and non-transgenic control Populus alba×P.glandulosa and the soil samples f...In this study,genetic stability of foreign genes was checked by using PCR technology. A total of 4 lines of soil samples from transgenic and non-transgenic control Populus alba×P.glandulosa and the soil samples from the stands were collected in two successive years,and the bacteria,fungi and antinomyces were isolated by using flat dilution method to determine their quantities. The results demonstrated that target genes were stable present in the genome of transgenic poplar. We found that three kinds of soil microorganism appeared different trends within different months and different years. The ANOVA and multiple comparison analyses revealed that there was no significant difference in quantity of the soil microorganisms among most poplar lines at the same time point. Meanwhile,significant differences of the soil microorganism community were found among several transgenic lines,and between a few transgenic and non-transgenic lines,but they did not show any relation to the type and number of foreign genes. Our preliminary results indicated that the 2-year-old transgenic poplar growing in field had no significant effects on soil microorganisms.展开更多
The oxygen evolution, thermal dissipation, and photochemical energy storage of three hybrid poplar clones, namely the triploid clone B342, the diploid clone B11 [(Populus alba×P. glandulosa)×(P.tomentosa...The oxygen evolution, thermal dissipation, and photochemical energy storage of three hybrid poplar clones, namely the triploid clone B342, the diploid clone B11 [(Populus alba×P. glandulosa)×(P.tomentosa×P.bolleana)], and the triploid clone B346 [(P.tomentosa×P. bolleana)×(P. alba×P.glandulosa)], under light stress were studied using photoacoustics. The oxygen evolution signal and photochemical energy storage varied negatively with the pretreatment_PFD (photon flux density), whereas the thermal signal varied positively with the pretreatment_PFD. Photochemical energy storage was reallocated to PSⅡ more than to PSⅠ, while the photochemical energy storage in PSⅠ was more stable than that in PSⅡ when subjected to light stress. The inhibitors streptomycin (SM), dithiothreitol (DTT) and sodium fluoride (NaF) could all affect the oxygen evolution signal. Clones B11 and B342 were more resistant to light stress than clone B346.展开更多
The study of green mass nutritional value of the black poplars euramerican hybrids--Populus euramericana （Dode） Guinier was conducted. The content of fodder units, digestible and crude protein, crude fat, fiber, ash...The study of green mass nutritional value of the black poplars euramerican hybrids--Populus euramericana （Dode） Guinier was conducted. The content of fodder units, digestible and crude protein, crude fat, fiber, ash, nitrogen, calcium, phosphorus, carotene and others was studied. The study showed that the leaves had greater nutritional value than stems and branches. The fresh leaves collected from the annual shoots of trees were more nutritious than annual coppice shoots of reproductive plantations but after drying the nutritional value of the materials became similar. Indicators of stems nutritional value were similar to those of wheat straw and those of leaves that were close to the nutritional value of alfalfa. Nutritional value of leafy shoots was intermediate between that of stems and leaves. Young coppice plant material is much easier to harvest than that of mature trees. Minirotation cultivation of poplars allows obtaining significant reserves of both wood and leafing biomass in a short period of time per unit area. The ＂old clones＂ of euramerican hybrids of black poplars are more winter resistant than some southern poplars of Italian breeding. This allows cultivating them not only in places of habitual areas of poplars, but also in more Northern regions.展开更多
文摘In this study,genetic stability of foreign genes was checked by using PCR technology. A total of 4 lines of soil samples from transgenic and non-transgenic control Populus alba×P.glandulosa and the soil samples from the stands were collected in two successive years,and the bacteria,fungi and antinomyces were isolated by using flat dilution method to determine their quantities. The results demonstrated that target genes were stable present in the genome of transgenic poplar. We found that three kinds of soil microorganism appeared different trends within different months and different years. The ANOVA and multiple comparison analyses revealed that there was no significant difference in quantity of the soil microorganisms among most poplar lines at the same time point. Meanwhile,significant differences of the soil microorganism community were found among several transgenic lines,and between a few transgenic and non-transgenic lines,but they did not show any relation to the type and number of foreign genes. Our preliminary results indicated that the 2-year-old transgenic poplar growing in field had no significant effects on soil microorganisms.
文摘The oxygen evolution, thermal dissipation, and photochemical energy storage of three hybrid poplar clones, namely the triploid clone B342, the diploid clone B11 [(Populus alba×P. glandulosa)×(P.tomentosa×P.bolleana)], and the triploid clone B346 [(P.tomentosa×P. bolleana)×(P. alba×P.glandulosa)], under light stress were studied using photoacoustics. The oxygen evolution signal and photochemical energy storage varied negatively with the pretreatment_PFD (photon flux density), whereas the thermal signal varied positively with the pretreatment_PFD. Photochemical energy storage was reallocated to PSⅡ more than to PSⅠ, while the photochemical energy storage in PSⅠ was more stable than that in PSⅡ when subjected to light stress. The inhibitors streptomycin (SM), dithiothreitol (DTT) and sodium fluoride (NaF) could all affect the oxygen evolution signal. Clones B11 and B342 were more resistant to light stress than clone B346.
文摘The study of green mass nutritional value of the black poplars euramerican hybrids--Populus euramericana （Dode） Guinier was conducted. The content of fodder units, digestible and crude protein, crude fat, fiber, ash, nitrogen, calcium, phosphorus, carotene and others was studied. The study showed that the leaves had greater nutritional value than stems and branches. The fresh leaves collected from the annual shoots of trees were more nutritious than annual coppice shoots of reproductive plantations but after drying the nutritional value of the materials became similar. Indicators of stems nutritional value were similar to those of wheat straw and those of leaves that were close to the nutritional value of alfalfa. Nutritional value of leafy shoots was intermediate between that of stems and leaves. Young coppice plant material is much easier to harvest than that of mature trees. Minirotation cultivation of poplars allows obtaining significant reserves of both wood and leafing biomass in a short period of time per unit area. The ＂old clones＂ of euramerican hybrids of black poplars are more winter resistant than some southern poplars of Italian breeding. This allows cultivating them not only in places of habitual areas of poplars, but also in more Northern regions.
