杂种甜瓜的离体快速繁殖

研究了快速繁殖杂种甜瓜“西莫洛托”试管苗的方法。结果表明，由无菌苗诱导不定芽，应先将无菌苗于 Ｍ Ｓ＋ Ｂ Ａ２．０ ｍ ｇ／ Ｌ＋ Ｎ Ａ Ａ０．２ ｍ ｇ／ Ｌ 培养基培养 ４ ｄ 后，再将其培养于 Ｍ Ｓ＋ Ｂ Ａ０．３ ｍ ｇ／ Ｌ培养基可获得较好的效果。不定芽的增殖，以选用 Ｍ Ｓ＋ Ｂ Ａ０．３ ｍ ｇ／ Ｌ 培养基效果最好，１５ ｄ 后繁殖系数达３．８。腋芽的诱导，以带叶柄的茎段培养于含低浓度 Ｂ Ａ 的培养基为佳。试管苗的生根，在 Ｎ Ａ Ａ、 Ｉ Ｂ Ａ、 Ｉ Ａ Ａ 三种生长素中，以 Ｎ Ａ Ａ 最适宜，１６ ｄ 后生根率达１００％ 。

杂种甜瓜组培快繁技术

研究了组培快繁杂种甜瓜“翠蜜”的方法。在甜瓜无菌苗上切取带子叶顶芽 ,进行不定芽诱导成苗 ,采用 MS+ (0 .3～ 0 .5 ) ml/L 6- BA+ (0 .1～ 0 .2 ) mg/L NAA培养基可获得较好的不定芽增殖效果 ;以切取甜瓜无菌苗带腋芽的茎段 ,在 MS+ 0 .1ml/L 6- BA+ (0～ 0 .1) mg/L NAA培养基上进行腋芽诱导成苗的诱导效果最佳。综合比较 ,腋芽诱导是甜瓜组培快繁比较理想的途径。

Interspecific Hybridization between Cucumis ssp. Plants and SRAP Analysis of Hybrids

To understand the inter-hybridization between Cucumis ssp.plants,we used 150 melon varieties as female parents to cross with Cucumis metuliferus and Cucumis anguria.Only melon accessions V2 and V129 set fruits,but seeds from fruits V2（V129）×C.metuliferus were abortive.A few of seeds from the bottom of fruit V2（V129）×C.anguria were fertile.Sequence-related amplified polymorphism（SRAP）molecular markers were used to analyze the progenies of inter-specific hybridization between C.anguria and melon V129.One pair primer（E14/M2）was found effective in amplification on male parent characteristic bands from the hybrids,suggesting that some DNA exchange had happened between C.anguria and melon V129.This study provided data for analyzing the mechanism of inter-hybridization between Cucumis plants.