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乌达矿区高硫煤中菌藻类体的发现及意义 被引量:11
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作者 代世峰 任德贻 +4 位作者 唐跃刚 艾天杰 周强 马凤学 李宝春 《中国矿业大学学报》 EI CAS CSCD 北大核心 1999年第1期57-60,共4页
聚煤环境是高硫煤形成的主要控制因素,在成煤作用过程中,细菌和藻类的参与是高硫煤形成的关键所在.应用带能谱仪的扫描电镜(SEM-EDX)和透射电镜(TEM),在乌达矿区高硫煤9煤层和10煤层的基质镜质体中发现了黄铁矿化... 聚煤环境是高硫煤形成的主要控制因素,在成煤作用过程中,细菌和藻类的参与是高硫煤形成的关键所在.应用带能谱仪的扫描电镜(SEM-EDX)和透射电镜(TEM),在乌达矿区高硫煤9煤层和10煤层的基质镜质体中发现了黄铁矿化的杆状菌落,在无结构镜质体中发现了蓝藻胶鞘及其降解有机质,这些菌藻类体的发现表明低等生源在高硫煤形成过程中起了重要作用. 展开更多
关键词 杆状菌落 乌达矿区 高硫煤 菌藻类 煤矿床
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Involvement of two glycoside hydrolase family 19 members in colony morphotype and virulence in Flavobacterium columnare
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作者 张晓林 李楠 +2 位作者 秦婷 黄贝 聂品 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2017年第6期1511-1523,共13页
Flavobacterium columnare is the pathogenic agent of columnaris disease in aquaculture. Using a recently developed gene deletion strategy, two genes that encode the Glyco hydro_19 domain (GH19 domain) containing prot... Flavobacterium columnare is the pathogenic agent of columnaris disease in aquaculture. Using a recently developed gene deletion strategy, two genes that encode the Glyco hydro_19 domain (GH19 domain) containing proteins, ghd-1 and ghd-2, were deleted separately and together from the F. columnare G4 wild type strain. Surprisingly, the single-, Aghd-1 and Aghd-2, and double-gene mutants, Aghd-1 Aghd-2, all had rhizoid and non-rhizoid colony morphotypes, which we named Aghd-1, Aghd-2, Aghd-1 Aghd-2, and NAghd-1, NAghd-2, and NAghd-1 Aghd-2. However, chitin utilization was not detected in either these mutants or in the wild type. Instead, skimmed milk degradation was observed for the mutants and the wild type; the non-rhizoid strain NAghd-2 exhibited higher degradation activity as revealed by the larger transparent circle on the skimmed milk plate. Using zebrafish as the model organism, we found that non-rhizoid mutants had higher LDs0 values and were less virulent because zebrafish infected with these survived longer. Transcriptome analysis between the non-rhizoid and rhizoid colony morphotypes of each mutant, i.e., NAghd-1 versus (vs) Aghd-1, NAghd-2 vs Aghd-2, and NAghd-1 Aghd-2 vs Aghd-1 Aghd-2, revealed a large number of differentially expressed genes, among which 39 genes were common in three of the pairs compared. Although most of these genes encode hypothetical proteins, a few molecules such as phage tail protein, rhs element Vgr protein, thiol-activated cytolysin, and TonB-dependent outer membrane receptor precursor, expression of which was down-regulated in non-rhizoid mutants but up-regulated in rhizoid mutants, may play a role F. columnare virulence. 展开更多
关键词 Flavobacterium columnare GH 19 domain gene deletion rhizoid colony non-rhizoid colony
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