Application of Pectin Crude Extract from Krung Kha Mao Leaves （Cissampelos pareira L.） for Immobilization of Lactobacillus casei subsp, rhamnosus TISTR 108 and Lactobacillus delbrueckii subsp, bulgaricus TISTR 1339 on Lactic Acid Production

The research was first to perform the utilization of novel and cheap pectin crude extract from Krung Kha Mao leaves （Cissampelos pareira L.） to immobilize cells of Lactobacillus casei subsp, rhamnosus TISTR 108 and Lactobacillus delbrueckii subsp, bulgaricus TISTR 1339 for lactic acid production, the optimum condition of immobilized cells was produced significantly at 5% of probability, that the highest lactic acid has been 38.50 and 33.66 g/L in steady state of whey medium for 96 and 108 h, respectively. Both strains were immobilized by 4% （w/w） of pectin crude extract from Krung Kha Mao （KKM pectin） leaves, 1.52 mm inner diameters of silicone tube and 5% （v/v） inoculum for immobilization. Efficiency of lactic acid production was compared by immobilized cells ofL. casei subsp, rhamnosus TISTR 108 when KKM pectin, commercial citrus pectin, commercial apple pectin and sodium alginate were used as supporting materials to produce lactic acid 38.50, 38.76, 30.43 and 34.56 g/L, respectively, the productivity of lactic acid has been 0.40, 0.40, 0.36 and 0.36 g/L h, respectively.

Exchanges

China,Chile to Boost Ties Chinese Premier Li Keqiang,together with President Michelle Bachelet of Chile,attended the opening ceremony of the Economic and Trade Seminar marking the 45th anniversary of the establishment of China-Chile diplomatic relations and the 7th Meeting of the China-Chile Business Council in Santiago on May 26,2015.

引凤筑巢

2007年1O月30日6时30分,湖南岳阳县张谷英小学校长李利亚电话告诉我,湖南平江新私塾张志勇先生、徐林茜女士将来张谷英小学讲学。张谷英小学将吸收张志勇的办学理念,借鉴他的办学模式与方法,全力打造张谷英村的教育前景,期望我能前往助兴。平江新私塾张志勇先生的事迹,我早有所闻。张志勇先生大驾光临,我自然也求之不得。于是,欣然应允。