The study was conducted for the detection of Listeria monocytogenes bacteria from different sources （CSF and blood） obtained from patients in Pediatric hospital and from food sources like （yogurt, raw vegetables an...The study was conducted for the detection of Listeria monocytogenes bacteria from different sources （CSF and blood） obtained from patients in Pediatric hospital and from food sources like （yogurt, raw vegetables and raw milk, sausage）. Ten isolates were isolated from 150 specimens one of them from CSF and one isolate isolated from blood samples the others isolated from food specimens 6 isolates isolated from sausage and 2 from raw vegetables. Isolates were identified traditionally involved culture methods based on selective enrichment and plating followed by the characterization of Listeria spp. based on colony morphology, sugar fermentation and haemolytic properties, identification by Api listeria was done. Determine the isolates that produce biofilm by tissue culture plate method. The highest biofilm forming strains ofListeria monocytogenes isolates appear in No. （D10, El, E5 and E7） OD reading each of them is （0.13, 0.09, 0.11 and 0.19） respectively, the lowest or poor biofilm forming strains appear in No. （D11, D12, E2, E3, E4 and E6） that optical density （OD） reading are （0.04, 0.03, 0.05, 0.04, 0.05 and 0.03） respectively by comparing with control prepared in well （A12） that stained by crystal violate without putting any isolates and the OD reading is （0.003）. Confirmation by PCR was done only for four isolates that produce biofilm （D10 and El） that obtained from CSF and blood sample and for （E5 and E7） that obtained from food samples.展开更多
The microbial contamination of fish is the most important factor in assurance of food safety. The microflora on the surface of skin, gills and gut of fish is constantly under the influence of the water environment and...The microbial contamination of fish is the most important factor in assurance of food safety. The microflora on the surface of skin, gills and gut of fish is constantly under the influence of the water environment and this may cause colonization of microorganisms on fish. The goal of this study was to evaluate the bacterial contamination level on the skin, gills and gut of the European eel （Anguilla anguilla）, i.e., detecting total bacteria count （TBC） and Enterobacteriaceae counts, as well as Listeria spp, and Salmonella spp. in freshly caught fish. Among the three lakes, TBC on skin, gills and gut varied from 0.66 CFU/cm2 to 4.93 CFU/cm~, from 0.40 CFU/cm2 to 5.51 CFU/cm2 and from 0.30 CFU/cm2 to 6.37 CFU/cm2, respectively. Enterobacteriaceae count on skin, gills and gut was from 0 CFU/cm2 to 4.30 CFU/cm2, from 0 CFU/cm2 to 2.47 CFU/cm2 and from 0 CFU/cm2 to 1.72 CFU/cm2, respectively. The highest mean count of TBC on gills, skin and gut was found in samples from Sivers lake, while the lowest was in samples from Aluksne lake. Values among the lakes were significanty （P 〈 0.05） different. Also the highest mean counts of Enterobacteriaceae were found on gills and skin of eels from Sivers lake, but the highest count on gut was found in Usma lake. All tested samples were Listeria monocytogenes and Salmonella spp. negative. TBC and Enterobacteriaceae counts of skin, gills and gut were typical for wild fish in fresh water. Foodborne pathogens, such as Salmonella spp. and L. monocytogenes, have not been found in the present study, indicating that fish are safe for human consumption.展开更多
文摘The study was conducted for the detection of Listeria monocytogenes bacteria from different sources （CSF and blood） obtained from patients in Pediatric hospital and from food sources like （yogurt, raw vegetables and raw milk, sausage）. Ten isolates were isolated from 150 specimens one of them from CSF and one isolate isolated from blood samples the others isolated from food specimens 6 isolates isolated from sausage and 2 from raw vegetables. Isolates were identified traditionally involved culture methods based on selective enrichment and plating followed by the characterization of Listeria spp. based on colony morphology, sugar fermentation and haemolytic properties, identification by Api listeria was done. Determine the isolates that produce biofilm by tissue culture plate method. The highest biofilm forming strains ofListeria monocytogenes isolates appear in No. （D10, El, E5 and E7） OD reading each of them is （0.13, 0.09, 0.11 and 0.19） respectively, the lowest or poor biofilm forming strains appear in No. （D11, D12, E2, E3, E4 and E6） that optical density （OD） reading are （0.04, 0.03, 0.05, 0.04, 0.05 and 0.03） respectively by comparing with control prepared in well （A12） that stained by crystal violate without putting any isolates and the OD reading is （0.003）. Confirmation by PCR was done only for four isolates that produce biofilm （D10 and El） that obtained from CSF and blood sample and for （E5 and E7） that obtained from food samples.
文摘The microbial contamination of fish is the most important factor in assurance of food safety. The microflora on the surface of skin, gills and gut of fish is constantly under the influence of the water environment and this may cause colonization of microorganisms on fish. The goal of this study was to evaluate the bacterial contamination level on the skin, gills and gut of the European eel （Anguilla anguilla）, i.e., detecting total bacteria count （TBC） and Enterobacteriaceae counts, as well as Listeria spp, and Salmonella spp. in freshly caught fish. Among the three lakes, TBC on skin, gills and gut varied from 0.66 CFU/cm2 to 4.93 CFU/cm~, from 0.40 CFU/cm2 to 5.51 CFU/cm2 and from 0.30 CFU/cm2 to 6.37 CFU/cm2, respectively. Enterobacteriaceae count on skin, gills and gut was from 0 CFU/cm2 to 4.30 CFU/cm2, from 0 CFU/cm2 to 2.47 CFU/cm2 and from 0 CFU/cm2 to 1.72 CFU/cm2, respectively. The highest mean count of TBC on gills, skin and gut was found in samples from Sivers lake, while the lowest was in samples from Aluksne lake. Values among the lakes were significanty （P 〈 0.05） different. Also the highest mean counts of Enterobacteriaceae were found on gills and skin of eels from Sivers lake, but the highest count on gut was found in Usma lake. All tested samples were Listeria monocytogenes and Salmonella spp. negative. TBC and Enterobacteriaceae counts of skin, gills and gut were typical for wild fish in fresh water. Foodborne pathogens, such as Salmonella spp. and L. monocytogenes, have not been found in the present study, indicating that fish are safe for human consumption.
