[Objective] The study was to explore the molecular interpretation standards on parentage in the seeds of corn variety. [Method] With 16 hybrids and their parents and 202 inbred elites as materials for screening primer...[Objective] The study was to explore the molecular interpretation standards on parentage in the seeds of corn variety. [Method] With 16 hybrids and their parents and 202 inbred elites as materials for screening primers, the artificial groups of two standard diad and two standard triad were respectively established as the verification materials. Genomic DNA of seedlings was extracted by using CTAB method. 137 pairs of SSR primers were selected for SSR amplification and product detection, which was used for the parentage identification of maize varieties. [ Result] Twenty pairs of corn primers with high polymorphism information content （ PIC value）, clearly amplified bands and good reproducibility were screened from 137 pairs of corn SSR primers tested. The identification results of using SSR molecular were consistent with the actual situation. [ Conclusion] It is feasible to identify the parentage of maize variety using SSR markers.展开更多
With the deep researches on male sterility genetic engineering of plants, several strategies creating male sterile materials have been developed, such as causing pollen abortion by cytotoxic genes, antisense RNA or RN...With the deep researches on male sterility genetic engineering of plants, several strategies creating male sterile materials have been developed, such as causing pollen abortion by cytotoxic genes, antisense RNA or RNAi silencing the expression of genes related to pollen development, early degradation of tapetum callose leading to male sterility. Male sterile transgenic plants can be obtained through genetic transformation with related genes destroying or interfering with pollen or anther development. Male sterile cauliflower, tomato, cabbage, etc. have been developed in this way, and some begin to be used to produce hybrid seed. Appling some techniques can also maintain and restore the male sterility. These related researches will effectively promote the heterosis utilization and the development of crop breeding. This paper mainly presents their principles and applications in vegetable crops.展开更多
基金Supported by the National Standard Plan(20051079-T-469)~~
文摘[Objective] The study was to explore the molecular interpretation standards on parentage in the seeds of corn variety. [Method] With 16 hybrids and their parents and 202 inbred elites as materials for screening primers, the artificial groups of two standard diad and two standard triad were respectively established as the verification materials. Genomic DNA of seedlings was extracted by using CTAB method. 137 pairs of SSR primers were selected for SSR amplification and product detection, which was used for the parentage identification of maize varieties. [ Result] Twenty pairs of corn primers with high polymorphism information content （ PIC value）, clearly amplified bands and good reproducibility were screened from 137 pairs of corn SSR primers tested. The identification results of using SSR molecular were consistent with the actual situation. [ Conclusion] It is feasible to identify the parentage of maize variety using SSR markers.
文摘With the deep researches on male sterility genetic engineering of plants, several strategies creating male sterile materials have been developed, such as causing pollen abortion by cytotoxic genes, antisense RNA or RNAi silencing the expression of genes related to pollen development, early degradation of tapetum callose leading to male sterility. Male sterile transgenic plants can be obtained through genetic transformation with related genes destroying or interfering with pollen or anther development. Male sterile cauliflower, tomato, cabbage, etc. have been developed in this way, and some begin to be used to produce hybrid seed. Appling some techniques can also maintain and restore the male sterility. These related researches will effectively promote the heterosis utilization and the development of crop breeding. This paper mainly presents their principles and applications in vegetable crops.
