[ Objective] The experiment aimed to clone Paramyosin/mini-Paramyosin （PM/mPM） gene to analyze the relations between it and moving behaviors. [Method] PCR method and RACE technology were used to obtain whole cDNA of...[ Objective] The experiment aimed to clone Paramyosin/mini-Paramyosin （PM/mPM） gene to analyze the relations between it and moving behaviors. [Method] PCR method and RACE technology were used to obtain whole cDNA of PM and some cDNA of mPM of Bombyx moil. By comparing wgs of Bombyx mori, the genomic sequence of PM/mPM of Bombyx mori was obtained, and, their genome structure was determined. [ Result] PM/mPM genes consisted of 17 exons and 16 intrens. By the use of selective promoters, The gene sequence encoded PM and mPM, while PM and mPM shared the last 6 exons. The cluster analysis between PM of Bombyx mori and PM of other invertebrate animals demonstrated that the relation between Bombyx mori and Bombyx mandarina was closest and the relation between Bombyx mori and Drosophila melanogaster was farthest in Insecta. [ Conclusion] There was no point mutation which could influence flight in amino acid sequence of PM of Bombyx mori and Bombyx mandarina, so the difference of flight capacity of Bombyx mori and Bombyx mandarina might be regulated by other mechanism.展开更多
Graphene oxide(GO)has demonstrated potential applications in various fields,and attracted intensive attention in industry as well.Numerous companies worldwide have been working on the industrial applications of GO-bas...Graphene oxide(GO)has demonstrated potential applications in various fields,and attracted intensive attention in industry as well.Numerous companies worldwide have been working on the industrial applications of GO-based materials in,e.g.,thermal management,multifunctional composites,anti-corrosion paints,lubricants,energy storage,environment protection and biomedicals.This review presents a short summary on the proceedings of GO towards industrialization,including the large-scale production and some promising applications,by providing views on the processing strategies and challenges specifically for the industrial use of GO.This review would help the scientists in this area to find topics for overcoming challenges together with engineers.展开更多
基金Supported by the National High Technology Research and Development Program of China(863 Program)(No.2006AA10A118)~~
文摘[ Objective] The experiment aimed to clone Paramyosin/mini-Paramyosin （PM/mPM） gene to analyze the relations between it and moving behaviors. [Method] PCR method and RACE technology were used to obtain whole cDNA of PM and some cDNA of mPM of Bombyx moil. By comparing wgs of Bombyx mori, the genomic sequence of PM/mPM of Bombyx mori was obtained, and, their genome structure was determined. [ Result] PM/mPM genes consisted of 17 exons and 16 intrens. By the use of selective promoters, The gene sequence encoded PM and mPM, while PM and mPM shared the last 6 exons. The cluster analysis between PM of Bombyx mori and PM of other invertebrate animals demonstrated that the relation between Bombyx mori and Bombyx mandarina was closest and the relation between Bombyx mori and Drosophila melanogaster was farthest in Insecta. [ Conclusion] There was no point mutation which could influence flight in amino acid sequence of PM of Bombyx mori and Bombyx mandarina, so the difference of flight capacity of Bombyx mori and Bombyx mandarina might be regulated by other mechanism.
基金This work was supported by the National Natural Science Foundation of China(51772282).
文摘Graphene oxide(GO)has demonstrated potential applications in various fields,and attracted intensive attention in industry as well.Numerous companies worldwide have been working on the industrial applications of GO-based materials in,e.g.,thermal management,multifunctional composites,anti-corrosion paints,lubricants,energy storage,environment protection and biomedicals.This review presents a short summary on the proceedings of GO towards industrialization,including the large-scale production and some promising applications,by providing views on the processing strategies and challenges specifically for the industrial use of GO.This review would help the scientists in this area to find topics for overcoming challenges together with engineers.
