目的验证钴60-γ射线辐照对动物源性膜材中模拟感染指示病毒的灭活效果。方法将3个连续批次的动物源性膜材中间品,按1∶10(m/V)加入预先测定滴度的CVB3、PRV、BVDV和PPV病毒。设立未经辐照的病毒为对照组,试验组经25 k Gy钴60-γ射线辐...目的验证钴60-γ射线辐照对动物源性膜材中模拟感染指示病毒的灭活效果。方法将3个连续批次的动物源性膜材中间品,按1∶10(m/V)加入预先测定滴度的CVB3、PRV、BVDV和PPV病毒。设立未经辐照的病毒为对照组,试验组经25 k Gy钴60-γ射线辐照后,提取浸提液,采用96孔板细胞病变(CPE)法观察各梯度稀释液的细胞病变情况,按照Reed-Muench法计算对照组和试验组中4种指示病毒的lg TCID50/0.1 ml,并对比辐照前后病毒滴度的变化情况。结果经25 k Gy钴60-γ射线辐照预先感染病毒的动物源性膜材后,实验结果显示4种指示病毒均不能使宿主细胞出现病变,各病毒滴度对数值变化均>4 logs,符合国家相关法规对动物源性材料病毒灭活效果的要求。结论25 k Gy钴60-γ射线辐照可以作为灭活动物源性膜材中潜在病毒的有效方法。展开更多
China West International Holdings Limited is an Australian listed company.It specialized in in- dustrial investments in construction materials,energy development,infrastructure,etc.At present, China West International...China West International Holdings Limited is an Australian listed company.It specialized in in- dustrial investments in construction materials,energy development,infrastructure,etc.At present, China West International Holdings Limited wholly controls Yuan Construction and sale of common Portland cement,limestone mining,etc.,also controls advanced technology companies.Wholly owns Chongqing Sino-Australia Waterproof Material Co.,Ltd.展开更多
文摘目的验证钴60-γ射线辐照对动物源性膜材中模拟感染指示病毒的灭活效果。方法将3个连续批次的动物源性膜材中间品,按1∶10(m/V)加入预先测定滴度的CVB3、PRV、BVDV和PPV病毒。设立未经辐照的病毒为对照组,试验组经25 k Gy钴60-γ射线辐照后,提取浸提液,采用96孔板细胞病变(CPE)法观察各梯度稀释液的细胞病变情况,按照Reed-Muench法计算对照组和试验组中4种指示病毒的lg TCID50/0.1 ml,并对比辐照前后病毒滴度的变化情况。结果经25 k Gy钴60-γ射线辐照预先感染病毒的动物源性膜材后,实验结果显示4种指示病毒均不能使宿主细胞出现病变,各病毒滴度对数值变化均>4 logs,符合国家相关法规对动物源性材料病毒灭活效果的要求。结论25 k Gy钴60-γ射线辐照可以作为灭活动物源性膜材中潜在病毒的有效方法。
文摘China West International Holdings Limited is an Australian listed company.It specialized in in- dustrial investments in construction materials,energy development,infrastructure,etc.At present, China West International Holdings Limited wholly controls Yuan Construction and sale of common Portland cement,limestone mining,etc.,also controls advanced technology companies.Wholly owns Chongqing Sino-Australia Waterproof Material Co.,Ltd.
