Objectives To identify the 5'untranslated region of Zika virus(ZIKV 5'UTR)RNA-binding proteins and to investigate the impact of the binding protein on the activity of internal ribosomal entry site(IRES)located...Objectives To identify the 5'untranslated region of Zika virus(ZIKV 5'UTR)RNA-binding proteins and to investigate the impact of the binding protein on the activity of internal ribosomal entry site(IRES)located in ZIKV 5'UTR and virus production.Methods Interacting proteins in U251 cells were captured using tRSA-tagged ZIKV 5'UTR RNA and tRSA-ZIKV 5'UTR RNA-binding proteins were visualized by SDS-PAGE silver staining,Subsequently,liquid chromatographytandem mass spectrometry(LC-MS/MS),bioinformatics analysis,and Western blot were used to identify the candidate proteins binding to ZIKV 5'UTR.Dicistronic expression assay and plaque forming assay were performed to analyze the effect of the binding protein on ZIKV IRES activity and ZIKV production,respecitvely.Results tRSA RNA pull-down assay,LC-MS/MS,and Western blot analysis showed that polypyrimidine tractbinding protein(PTB)bound to the ZIKV 5'UTR.Furthermore,dual luciferase reporter assay revealed that overexpression of PTB significantly enhanced the IRES activity of ZIKV(t=10.220,P<0.001),while PTB knockdown had the opposite effect(t=4.897,P<0.01).Additionally,virus plaque forming assay demonstrated that up-regulation of PTB expression significantly enhanced viral titer(t=6.400,P<0.01),whereas reducing PTB expression level weakened virus infectivity(t=5.055,P<0.01).Conclusion PTB positively interacts with the ZIKV 5'UTR and enhances IRES activity and virus production.展开更多
Si based single electron transistor (SET) is fabricated successfully on p type SIMOX substrate,based on electron beam (EB) lithography,reactive ion etching (RIE) and thermal oxidation.In particular,using thermal oxi...Si based single electron transistor (SET) is fabricated successfully on p type SIMOX substrate,based on electron beam (EB) lithography,reactive ion etching (RIE) and thermal oxidation.In particular,using thermal oxidation and etching off the oxide layer,a one dimensional Si quantum wire can be converted into several quantum dots inside quantum wire in connection with the source and drain regions.The differential conductance (d I ds /d V ds ) oscillations and the Coulomb staircases in the source drain current ( I ds ) are shown clearly dependent on the source drain voltage at 5 3K.The I ds V gs (gate voltage) oscillations are observed from the I ds V gs characteristics as a function of V gs at different temperatures and various values of V ds .For a SET whose total capacitance is about 9 16aF,the I ds V gs oscillations can be observed at 77K.展开更多
A rearrangeable nonblocking silicon-on-insulator-based thermo-optic 4x4 switch matrix with spot size converters (SSCs) and a new driving circuit are designed and fabricated. The introduction of a spot size converter...A rearrangeable nonblocking silicon-on-insulator-based thermo-optic 4x4 switch matrix with spot size converters (SSCs) and a new driving circuit are designed and fabricated. The introduction of a spot size converter (SSC) has decreased the insertion loss to less than 10dB and the new driving circuit has improved the response speed to less than l^s.展开更多
The properties of a bound polaron in a parabolic quantum dot with weak electron-LO-phonon coupling under a Coulomb field are studied. The ground state energy of the bound polaron is derived by using a linear combinati...The properties of a bound polaron in a parabolic quantum dot with weak electron-LO-phonon coupling under a Coulomb field are studied. The ground state energy of the bound polaron is derived by using a linear combination operator and the perturbation method. The influence of the interaction between phonons with different wave vectors in the recoil process on the ground state energy of the bound polaron is discussed. Numerical calculations are performed,and the results show that the ground state energy increases significantly as the effective confinement length of the quantum dot decreases,considering of the interaction between phonons. When l0〉1.0, the influence of the interaction between phonons on the ground state energy cannot be ignored.展开更多
In this paper, a novel method of laser beam deflecting is introduced to improve the spatial resolution of an acoustic-optic deflector (AOD). We use double AOD (DAOD) to control laser beam. Compared with single AOD, th...In this paper, a novel method of laser beam deflecting is introduced to improve the spatial resolution of an acoustic-optic deflector (AOD). We use double AOD (DAOD) to control laser beam. Compared with single AOD, the double AOD can improve speed and precision of target tracking and pointing significantly. Finally, a method of calculating the static and dynamic number of resolvable points (NRP) is provided, and it is supported by the experimental data excellently.展开更多
To solve problems in small-scale and complex structural traps,the inverse Gaussian-beam stack-imaging method is commonly used to process crosswell seismic wave reflection data.Owing to limited coverage,the imaging qua...To solve problems in small-scale and complex structural traps,the inverse Gaussian-beam stack-imaging method is commonly used to process crosswell seismic wave reflection data.Owing to limited coverage,the imaging quality of conventional ray-based crosswell seismic stack imaging is poor in complex areas;moreover,the imaging range is small and with sever interference because of the arc phenomenon in seismic migration.Thus,we propose the inverse Gaussian-beam stack imaging,in which Gaussian weight functions of rays contributing to the geophones energy are calculated and used to decompose the seismic wavefield.This effectively enlarges the coverage of the reflection points and improves the transverse resolution.Compared with the traditional VSP–CDP stack imaging,the proposed methods extends the imaging range,yields higher horizontal resolution,and is more adaptable to complex geological structures.The method is applied to model a complex structure in the K-area.The results suggest that the wave group of the target layer is clearer,the resolution is higher,and the main frequency of the crosswell seismic section is higher than that in surface seismic exploration The effectiveness and robustness of the method are verified by theoretical model and practical data.展开更多
Wang Wei-fan[1] proved that the edge-face chromatic number of a 2-connected 1-tree with the maximum degree is not less than 6 is its maximum degree, and he conjectured that it is true when the maximum degree is 5. Thi...Wang Wei-fan[1] proved that the edge-face chromatic number of a 2-connected 1-tree with the maximum degree is not less than 6 is its maximum degree, and he conjectured that it is true when the maximum degree is 5. This paper proves the conjecture.展开更多
Self-organized In 0.5 Ga 0.5 As/GaAs quantum island structure emitting at 1.35 μm at room temperature has been successfully fabricated by molecular beam epitaxy (MBE) via cycled (InAs) 1/(GaAs) 1 monolayer deposition...Self-organized In 0.5 Ga 0.5 As/GaAs quantum island structure emitting at 1.35 μm at room temperature has been successfully fabricated by molecular beam epitaxy (MBE) via cycled (InAs) 1/(GaAs) 1 monolayer deposition method. Photoluminescence (PL) measurement shows that very narrow PL linewidth of 19.2 meV at 300 K has been reached for the first time, indicating effective suppression of inhomogeneous broadening of optical emission from the In 0.5 Ga 0.5 As islands structure. Our results provide important information for optimizing the epitaxial structures of 1.3 μm wavelength quantum dot (QD) devices.展开更多
An effective spot beam handover trigger and channel allocation scheme is proposed for GEO mobile satellite communication based on its characteristic and application. By using both signal strength and terminal location...An effective spot beam handover trigger and channel allocation scheme is proposed for GEO mobile satellite communication based on its characteristic and application. By using both signal strength and terminal location information, necessary handover is triggered promptly and accurately to reduce the negative effect of long signaling delay. Then handover decision is made with the handover queuing and channel allocation strategy. An adaptive channel resource allocation scheme is considered to optimize resource utilization with guarantee of emergency communication, which is significant for emergency rescue and disaster relief. Simulation results show that the proposed scheme prevents unnecessary handover effectively and has favorable adaptability to emergent requirement of satellite communication.展开更多
The problem of correcting simultaneously mass and stiffness matrices of finite element model of undamped structural systems using vibration tests is considered in this paper.The desired matrix properties,including sat...The problem of correcting simultaneously mass and stiffness matrices of finite element model of undamped structural systems using vibration tests is considered in this paper.The desired matrix properties,including satisfaction of the characteristic equation,symmetry,positive semidefiniteness and sparsity,are imposed as side constraints to form the optimal matrix pencil approximation problem.Using partial Lagrangian multipliers,we transform the nonlinearly constrained optimization problem into an equivalent matrix linear variational inequality,develop a proximal point-like method for solving the matrix linear variational inequality,and analyze its global convergence.Numerical results are included to illustrate the performance and application of the proposed method.展开更多
The authors used Landau-Pekar variational method to investigate a strong-coupling singlet optical bipolaron in different configuration of quantum confinement. Numerical and analytical results showed that when configur...The authors used Landau-Pekar variational method to investigate a strong-coupling singlet optical bipolaron in different configuration of quantum confinement. Numerical and analytical results showed that when configuration changes from quantum dot and wire to well, confinement shows different effect on the formation of a bipolaron. In contrast to a bipolaron in a quantum dot or wire, the binding energy of a bipolaron in a quantum well increases with increasing con-finement, indicating that confinement favors bipolaron formation in a quantum well.展开更多
this paper,we propose a class of smoothing-regularization methods for solving the mathematical programming with vanishing constraints.These methods include the smoothing-regularization method proposed by Kanzow et al....this paper,we propose a class of smoothing-regularization methods for solving the mathematical programming with vanishing constraints.These methods include the smoothing-regularization method proposed by Kanzow et al.in[Comput.Optim.Appl.,2013,55(3):733-767]as a special case.Under the weaker conditions than the ones that have been used by Kanzow et al.in 2013,we prove that the Mangasarian-Fromovitz constraint qualification holds at the feasible points of smoothing-regularization problem.We also analyze that the convergence behavior of the proposed smoothing-regularization method under mild conditions,i.e.,any accumulation point of the stationary point sequence for the smoothing-regularization problem is a strong stationary point.Finally,numerical experiments are given to show the efficiency of the proposed methods.展开更多
Sparse optimization has witnessed advancements in recent decades,and the step function finds extensive applications across various machine learning and signal processing domains.This paper integrates zero norm and the...Sparse optimization has witnessed advancements in recent decades,and the step function finds extensive applications across various machine learning and signal processing domains.This paper integrates zero norm and the step function to formulate a doublesparsity constrained optimization problem,wherein a linear equality constraint is also taken into consideration.By defining aτ-Lagrangian stationary point and a KKT point,we establish the first-order and second-order necessary and sufficient optimality conditions for the problem.Furthermore,we thoroughly elucidate their relationships to local and global optimal solutions.Finally,special cases and examples are presented to illustrate the obtained theorems.展开更多
National policies and legal decisions are very much dependent on the position of the shoreline. Shoreline change rates are frequently employed to summarize historical shoreline movements. This also helps to predict th...National policies and legal decisions are very much dependent on the position of the shoreline. Shoreline change rates are frequently employed to summarize historical shoreline movements. This also helps to predict the future position of the shoreline based on the perceived historical trends. In this regard, the future shoreline positions at both the long-term, that is 2050, and short-term, that is 2015, time interval was predicted using the End Point Rate (EPR) model along the Junput Coast of West Bengal, India. The whole project work was divided into five parts. The first part showed the detection of shoreline from satellite data like IRS LISS IV and Landsat 7 ETM+ and from the Survey of India Toposheet. The second part gave the glimpse of the dynamic segmentation of the shoreline to get the dynamically segmented nodal points along the shoreline. Shoreline prediction for the years 2015 and 2050 using End Point Rate (EPR) model was done in the third part. In the fourth part, Coastal Terrain Model (CTM) was prepared, and the digital shoreline estimated. The model result was validated and accuracy assessed with respect to the GPS data collected from the field at the fifth stage. Finally at the end of the present work, limitations of the project and the future scope of the work was sited.展开更多
文摘Objectives To identify the 5'untranslated region of Zika virus(ZIKV 5'UTR)RNA-binding proteins and to investigate the impact of the binding protein on the activity of internal ribosomal entry site(IRES)located in ZIKV 5'UTR and virus production.Methods Interacting proteins in U251 cells were captured using tRSA-tagged ZIKV 5'UTR RNA and tRSA-ZIKV 5'UTR RNA-binding proteins were visualized by SDS-PAGE silver staining,Subsequently,liquid chromatographytandem mass spectrometry(LC-MS/MS),bioinformatics analysis,and Western blot were used to identify the candidate proteins binding to ZIKV 5'UTR.Dicistronic expression assay and plaque forming assay were performed to analyze the effect of the binding protein on ZIKV IRES activity and ZIKV production,respecitvely.Results tRSA RNA pull-down assay,LC-MS/MS,and Western blot analysis showed that polypyrimidine tractbinding protein(PTB)bound to the ZIKV 5'UTR.Furthermore,dual luciferase reporter assay revealed that overexpression of PTB significantly enhanced the IRES activity of ZIKV(t=10.220,P<0.001),while PTB knockdown had the opposite effect(t=4.897,P<0.01).Additionally,virus plaque forming assay demonstrated that up-regulation of PTB expression significantly enhanced viral titer(t=6.400,P<0.01),whereas reducing PTB expression level weakened virus infectivity(t=5.055,P<0.01).Conclusion PTB positively interacts with the ZIKV 5'UTR and enhances IRES activity and virus production.
文摘Si based single electron transistor (SET) is fabricated successfully on p type SIMOX substrate,based on electron beam (EB) lithography,reactive ion etching (RIE) and thermal oxidation.In particular,using thermal oxidation and etching off the oxide layer,a one dimensional Si quantum wire can be converted into several quantum dots inside quantum wire in connection with the source and drain regions.The differential conductance (d I ds /d V ds ) oscillations and the Coulomb staircases in the source drain current ( I ds ) are shown clearly dependent on the source drain voltage at 5 3K.The I ds V gs (gate voltage) oscillations are observed from the I ds V gs characteristics as a function of V gs at different temperatures and various values of V ds .For a SET whose total capacitance is about 9 16aF,the I ds V gs oscillations can be observed at 77K.
基金Supported in part by the National Key Basic Research Special Foundation of China under Grant No. G2000-03-66the Na-tional High Technology Program of China under Grant No.2002AA312060the National Natural Science Foundation ofChina under Grant No. 60336010.
文摘A rearrangeable nonblocking silicon-on-insulator-based thermo-optic 4x4 switch matrix with spot size converters (SSCs) and a new driving circuit are designed and fabricated. The introduction of a spot size converter (SSC) has decreased the insertion loss to less than 10dB and the new driving circuit has improved the response speed to less than l^s.
文摘The properties of a bound polaron in a parabolic quantum dot with weak electron-LO-phonon coupling under a Coulomb field are studied. The ground state energy of the bound polaron is derived by using a linear combination operator and the perturbation method. The influence of the interaction between phonons with different wave vectors in the recoil process on the ground state energy of the bound polaron is discussed. Numerical calculations are performed,and the results show that the ground state energy increases significantly as the effective confinement length of the quantum dot decreases,considering of the interaction between phonons. When l0〉1.0, the influence of the interaction between phonons on the ground state energy cannot be ignored.
文摘In this paper, a novel method of laser beam deflecting is introduced to improve the spatial resolution of an acoustic-optic deflector (AOD). We use double AOD (DAOD) to control laser beam. Compared with single AOD, the double AOD can improve speed and precision of target tracking and pointing significantly. Finally, a method of calculating the static and dynamic number of resolvable points (NRP) is provided, and it is supported by the experimental data excellently.
基金sponsored by the National Key R&D Plan Project(Grant No.2016YFC0303900)Natural Science Foundation of China(Grant No.41374145)
文摘To solve problems in small-scale and complex structural traps,the inverse Gaussian-beam stack-imaging method is commonly used to process crosswell seismic wave reflection data.Owing to limited coverage,the imaging quality of conventional ray-based crosswell seismic stack imaging is poor in complex areas;moreover,the imaging range is small and with sever interference because of the arc phenomenon in seismic migration.Thus,we propose the inverse Gaussian-beam stack imaging,in which Gaussian weight functions of rays contributing to the geophones energy are calculated and used to decompose the seismic wavefield.This effectively enlarges the coverage of the reflection points and improves the transverse resolution.Compared with the traditional VSP–CDP stack imaging,the proposed methods extends the imaging range,yields higher horizontal resolution,and is more adaptable to complex geological structures.The method is applied to model a complex structure in the K-area.The results suggest that the wave group of the target layer is clearer,the resolution is higher,and the main frequency of the crosswell seismic section is higher than that in surface seismic exploration The effectiveness and robustness of the method are verified by theoretical model and practical data.
文摘Wang Wei-fan[1] proved that the edge-face chromatic number of a 2-connected 1-tree with the maximum degree is not less than 6 is its maximum degree, and he conjectured that it is true when the maximum degree is 5. This paper proves the conjecture.
文摘Self-organized In 0.5 Ga 0.5 As/GaAs quantum island structure emitting at 1.35 μm at room temperature has been successfully fabricated by molecular beam epitaxy (MBE) via cycled (InAs) 1/(GaAs) 1 monolayer deposition method. Photoluminescence (PL) measurement shows that very narrow PL linewidth of 19.2 meV at 300 K has been reached for the first time, indicating effective suppression of inhomogeneous broadening of optical emission from the In 0.5 Ga 0.5 As islands structure. Our results provide important information for optimizing the epitaxial structures of 1.3 μm wavelength quantum dot (QD) devices.
基金Supported by the High Technology Research and Development Programme of China (No. 2008AA011102) and the National Natural Science Foundation of China ( No. 60772112).
文摘An effective spot beam handover trigger and channel allocation scheme is proposed for GEO mobile satellite communication based on its characteristic and application. By using both signal strength and terminal location information, necessary handover is triggered promptly and accurately to reduce the negative effect of long signaling delay. Then handover decision is made with the handover queuing and channel allocation strategy. An adaptive channel resource allocation scheme is considered to optimize resource utilization with guarantee of emergency communication, which is significant for emergency rescue and disaster relief. Simulation results show that the proposed scheme prevents unnecessary handover effectively and has favorable adaptability to emergent requirement of satellite communication.
基金The work was supported by the National Natural Science Foundation of China(No.11571171)。
文摘The problem of correcting simultaneously mass and stiffness matrices of finite element model of undamped structural systems using vibration tests is considered in this paper.The desired matrix properties,including satisfaction of the characteristic equation,symmetry,positive semidefiniteness and sparsity,are imposed as side constraints to form the optimal matrix pencil approximation problem.Using partial Lagrangian multipliers,we transform the nonlinearly constrained optimization problem into an equivalent matrix linear variational inequality,develop a proximal point-like method for solving the matrix linear variational inequality,and analyze its global convergence.Numerical results are included to illustrate the performance and application of the proposed method.
文摘The authors used Landau-Pekar variational method to investigate a strong-coupling singlet optical bipolaron in different configuration of quantum confinement. Numerical and analytical results showed that when configuration changes from quantum dot and wire to well, confinement shows different effect on the formation of a bipolaron. In contrast to a bipolaron in a quantum dot or wire, the binding energy of a bipolaron in a quantum well increases with increasing con-finement, indicating that confinement favors bipolaron formation in a quantum well.
基金Supported in part by NSFC(No.11961011)Guangxi Science and Technology Base and Talents Special Project(No.2021AC06001).
文摘this paper,we propose a class of smoothing-regularization methods for solving the mathematical programming with vanishing constraints.These methods include the smoothing-regularization method proposed by Kanzow et al.in[Comput.Optim.Appl.,2013,55(3):733-767]as a special case.Under the weaker conditions than the ones that have been used by Kanzow et al.in 2013,we prove that the Mangasarian-Fromovitz constraint qualification holds at the feasible points of smoothing-regularization problem.We also analyze that the convergence behavior of the proposed smoothing-regularization method under mild conditions,i.e.,any accumulation point of the stationary point sequence for the smoothing-regularization problem is a strong stationary point.Finally,numerical experiments are given to show the efficiency of the proposed methods.
基金Supported by the National Key R&D Program of China(No.2023YFA1011100)NSFC(No.12131004)。
文摘Sparse optimization has witnessed advancements in recent decades,and the step function finds extensive applications across various machine learning and signal processing domains.This paper integrates zero norm and the step function to formulate a doublesparsity constrained optimization problem,wherein a linear equality constraint is also taken into consideration.By defining aτ-Lagrangian stationary point and a KKT point,we establish the first-order and second-order necessary and sufficient optimality conditions for the problem.Furthermore,we thoroughly elucidate their relationships to local and global optimal solutions.Finally,special cases and examples are presented to illustrate the obtained theorems.
基金Supported by Indian Institute of Remote Sensing (ISRO), India and ITC, the Netherlands
文摘National policies and legal decisions are very much dependent on the position of the shoreline. Shoreline change rates are frequently employed to summarize historical shoreline movements. This also helps to predict the future position of the shoreline based on the perceived historical trends. In this regard, the future shoreline positions at both the long-term, that is 2050, and short-term, that is 2015, time interval was predicted using the End Point Rate (EPR) model along the Junput Coast of West Bengal, India. The whole project work was divided into five parts. The first part showed the detection of shoreline from satellite data like IRS LISS IV and Landsat 7 ETM+ and from the Survey of India Toposheet. The second part gave the glimpse of the dynamic segmentation of the shoreline to get the dynamically segmented nodal points along the shoreline. Shoreline prediction for the years 2015 and 2050 using End Point Rate (EPR) model was done in the third part. In the fourth part, Coastal Terrain Model (CTM) was prepared, and the digital shoreline estimated. The model result was validated and accuracy assessed with respect to the GPS data collected from the field at the fifth stage. Finally at the end of the present work, limitations of the project and the future scope of the work was sited.