《化玉诗草》出版发行暨杨化玉捐助家乡教育仪式在京举行

近几年来,出版个人文集的人不少见,但是出书和捐助放在一起的作家却不多见。中国艺术研究院党委副书记、副院长杨化玉多年来笔耕不辍,从读书到工作30多年以来创作古体诗、词。

杨化喜的作品

杨化喜中国美术家协会会员,贵州省美术家协会会员,1994年毕业于贵州大学艺术学院,2008年进修于广州美术学院油画系高研班,2014年进修于中国油画院,2015年进修于清华大学美术学院油画创作高研班,2017年于意大利学习欧洲古典油画。多次参加全国性油画大展并有部份获奖,多幅作品被国内专业美术馆收藏。

黑龙江马匹毛色遗传的调查研究

黑龙江马匹毛色遗传的调查研究杨化文（黑龙江省畜牧研究所１６１０４１）吴中友（黑龙江省国营九三农场管理局）刘成邦（大庆市三环公司）杨庆达，杨军达（黑龙江兽医科学研究所）虽然毛色对马匹的关系不大，但从品种的一致性、骏料测马群的整齐度以及防寒防热（深毛色耐...

关于患子宫炎症母马授精后洗治效果的探讨

关于患子宫炎症母马授精后洗治效果的探讨杨化文，程丽萍（黑龙江省畜牧研究所１６１０４１）杨庆达（黑龙江省兽医科学研究所）吴中有（黑龙江省九三农管局科研所）多年来，母马繁殖上最大障碍之一就是子宫炎症。在７０～８０年代，每年的发生率都在５％～８％之间，严重...

Studies of Transgenic Hybrid Poplar 741 Carrying Two Insect-resistant Genes

Partially modified Bt Cry1Ac gene and the arrowhead proteinase inhibitor (API) gene were used to construct a plant transformation vector pBtiA and this construct was transferred into the genome of the hybrid poplar 741 [ Populus alba L.×( P. davidiana Dode+ P. simonii Carr.)× P. tomentosa Carr.] by Agrobacterium _ mediated transformation. Ten kanamycin resistant plants have been regenerated. Upon insect bioassay using Clostera anachoreta (Fabricius), three of the examined plants were demonstrated to be highly resistant to the testing insects. The mortality of insect larvae on one plant was higher than 90% in 6 days after infestation and the growth of the survival larvae were seriously inhibited. Results of PCR and Southern blot analysis indicated that both Bt Cry1Ac gene and API gene were integrated as a single copy into the genomes of these three plants when Cry1Ac gene fragment was used as the probe. Protein dot blot immunoassay and ELISA analysis revealed that at least the Cry1Ac protein was produced in these three transgenic plants and the expression levels were estimated to be approximately 0.015% of the leaf total soluble protein. This is the first report on insect resistant transgenic hybrid poplar 741 that expresses two insecticidal protein genes.

Extraction of Total DNA from Populus euphratica Oliv. and Populus pruinosa Schrenk. by Improved CTAB Method

This study presented an improved CTAB method for extracting DNA from leaves of Populus euphratica Oliv. and Populus pruinosa Schrenk. based on the conventional CTAB method. The results showed that preventing DNA from browning is a key step to obtain the high-quality DNA during DNA extraction, and under the condition of grinding in the presence of liquid nitrogen, adding such three antioxidants as PVP dry powder, Vc and β-mercaptoethanol could prevent DNA from browning effectively. The total DNA extracted by the improved CTAB method was subjected to PCR detection which proved that it totally satisfied the requirements of subsequent study.

Optimization of Regeneration System of Leaf and Stem of Populus euramericana 108

[Objective] The aim was to optimize the culture conditions for asexual reproduction system of Populus euramericana 108.[Method] Orthogonal designs were adopted optimize the culture conditions of the regeneration system for direct differentiation from leaves and induced callus from stems of P.euramericana 108 aseptic seeding.[Result] Leaves of P.euramericana 108 directly regenerated and differentiated under illumination,while stem segments preferred to regenerate and differentiate through callus induction under illumination.The differentiation medium of adventitious buds from leaves was MS medium （agar 7.0 g/L,pH 6.0,sucrose 20 g/L） added with 0.6 mg/L 6-BA and 0.2 mg/L NAA; callus induction medium of stem segments was WPM solid medium added with 0.75 mg/L KT and 1.5 mg/L 2,4-D.Rooting induction medium for adventitious buds was WPM solid culture （sucrose 30 g/L） added with 2.0 mg/L IBA.[Conclusion] The culture conditions for regeneration system of differentiation from leaves and induced callus of stems were optimized,which provides basis for the construction of tissue culture and genetic transformation system.

Participation of H_2O_2 in Enhancement of Cold Chilling by Salicylic Acid in Banana Seedlings

The possible physiological mechanism of enhancement of cold tolerance by salicylic acid (SA) in banana seedlings ( Musa acuminata cv. Williams 8188) was explored. Measurements of leakage electrolyte after 2 d of recovery at 30/22 ℃ (day/night) following 3 d of cold stress at 7 ℃ showed that pretreatment with hydroponic solution containing SA 0.3-0.9 mmol/L as foliar spray under normal growth conditions (30/22 ℃) could significantly enhance cold tolerance of banana plants. The highest enhancing effect of SA occurred at 0.5 mmol/L and it showed the lowest leakage rate of electrolyte or smaller leaf wilting area after 2 d of recovery at normal temperature from 3 d of 7 ℃ or 5 ℃ cold stress. Higher concentrations (≥2.5 mmol/L) of SA, however, caused more electrolyte leakage, indicating that they aggravated chilling damage. Enhanced cold tolerance by SA could be related to H 2O 2 metabolism. Compared with water_treated seedlings (control), SA 0.5 mmol/L treatment inhibited activities of catalase (CAT) and ascorbate peroxidase (APX), increased peroxidase (POX) activity, but did not affect the activity of superoxide dismutase (SOD) under normal growth conditions, and these changes might lead to an accumulation of H 2O 2, whereas SA pretreatment enhanced the activities of CAT and APX, and reduced the increase in productions of H 2O 2 and thiobarbituric acid_reaction substances (TBARS) during subsequent 7 ℃ cold stress and recovery periods. Exogenous H 2O 2 treatments (1.5 -2.5 mmol/L) also increased cold tolerance of banana seedlings. Furthermore, pretreatment of banana seedlings with dimethylthiourea (a trap for H 2O 2) significantly inhibited cold tolerance induced by SA. These results suggested that endogenous H 2O 2 may be required for SA_enhanced cold tolerance. The significance of the interaction of SA, H 2O 2 and H 2O 2_metabolizing enzymes during cold stress has been discussed.

Nitric Oxide Production is Involved in Inhibition Growth of Salicylate at Chilling Temperature

[Objective] The aim was to investigate whether NO is involved in the inhibitory effect of salicylic acid on the growth at low temperature in Arabidopsis.[Method] The endogenous NO level in roots of wild-type and NahG mutant growing at 22 or 4 ℃ was measured with NO-specific probe DAF-FM DA staining method.[Result] NO level in plants growing at 4 ℃ for a long term could be increased with time;low temperature could induce NO production in wild-type and NahG mutant,and the NO level in NahG mutant with low level of salicylic acid was significantly lower than that in wild-type.[Conclusion] NO was involved in the inhibitory effect of salicylic acid on the growth of Arabidopsis at low temperature.

Comparison of the Effects of H_2O_2 and Salicylic Acid on Alternative Respiratory Pathway in Aged Potato Tuber Slices

The influences of exogenous H 2O 2 and salicylic acid (SA) treatments on the alternative respiratory pathway (ARP) in aged potato (Solanum tuberosum L.) tuber slices were compared. The results showed that both H 2O 2(5.0 mmol/L) and SA (0.1 mmol/L) treatments had a significant inducing effect on ARP capacity (V alt ) and its ratio to total respiration (V alt /V t) in potato slices aged for 24 h. With a monoclonal antibody against the alternative oxidase (AOX), Western blotting results showed that both H 2O 2 and SA treatments increased the AOX expression levels in aged potato tuber slices. However, the results of oxygen isotope discrimination experiments showed that H 2O 2 had no influence on the in vivo ARP activity (ρV alt ) and its contribution to V t(expressed as ρV alt /V t) in potato slices aged for 24 h, but SA had a significant influence on the ρV alt and ρV alt /V t values of the aged potato tuber slices. These results indicate that there are differences between the effects of H 2O 2 and SA on ARP in plant tissues. Both of them possess the ability to induce ARP capacity through inducing AOX expression. However, SA can simultaneously stimulate the operation of ARP, but H 2O 2 can not.

练出好体魄 适应快节奏

新乡机床厂举办第二届厂长长跑邀请赛,四市三十八个厂家一百三十名厂领导竞练铁脚板元月二十六日,新乡床厂,如庆大典.

Effects of Calcium Oxide on Physicochemical Properties of Spray-dried Bayberry Powder

[Objective] Effects of different contents of calcium oxide on physicochemi- cal properties of spray-dried bayberry powder were studied. [Method] Bayberry pow- der produced from bayberry juice adding 0, 2 and 4 g/L calcium oxide using the spray drying technique has been selected. The physicochemical properties of the spray-dried bayberry powder were investigated by determining moisture content, colour, apparent density, wettability, angle of repose and moisture absorption rate of bayberry powder. [Result] The results showed that the content of calcium oxide had a significant effect on the moisture content and colour of bayberry powder. Effects of different contents of calcium oxide on the apparent density of spray-dried bayber- ry powder were not obvious. Adding calcium oxide had a significant effect on the wettability, angle of repose and moisture absorption rate of spray-dried bayberry powder, nevertheless, there was not obvious difference on these properties between adding 2 and 4 g/L calcium oxide. [Conclusion] The results confirmed that the con- tent of calcium oxide had a significant effect on the properties of spray-dried bay- berry powder.

青年教师积极开展教研

我校目前教师中,35岁以下的青年教师有82人,占教师总人数的41.6％。学校非常重视青年教师的培养、深造,青年教师也普遍努力钻研教学,提高业务.

A magnetic biocatalyst based on mussel-inspired polydopamine and its acylation of dihydromyricetin

A support made of mussel-inspired polydopamine-coated magnetic iron oxide nanoparticles （PD-MNPs） was prepared and characterized. The widely used Aspetyillus niger lipase （ANL） was immobilized on the PD-MNPs （ANL@PD-MNPs） with a protein loading of 138 mg/g and an activity recovery of 83.6% under optimized conditions. For the immobilization, the pH and immobilization time were investigated. The pH and thermal and storage stability of the ANL@PD-MNPs significant- ly surpassed those of free ANL. The ANL@PD-MNPs had better solvent tolerance than free ANL. The secondary structure of free ANL and ANL@PD-MNPs was analyzed by infrared spectroscopy, A kinetic study demonstrated that the ANL@PD-MNPs had enhanced enzyme-substrate affinity and high catalytic efficiency. The ANL@PD-MNPs was applied as a biocatalyst for the regioselective acylation of dihydromyricetin （DMY） in DMSO and gave a conversion of 79.3%, which was higher than that of previous reports. The ANL@PD-MNPs retained over 55% of its initial activity after 10 cycles of reuse. The ANL@PD-MNPs were readily separated from the reaction system by a magnet. The PD-MNPs is an excellent support for ANL and the resulting ANL@PD-MNPs displayed good potential for the efficient synthesis of dihydromyricetin-3-acetate by enzymatic regioselective acylation.

EAG and behavioral responses of Helicoverpa armigera males to volatiles from poplar leaves and their combinations with sex pheromone

Electroantennogram (EAG) evaluation of selected compounds from wilted leaves of black poplar, Populus nigra, showed that phenyl acetaldehyde, methyl salicylate, (E)-2-hexenal elicited strong responses from male antennae of Helicoverpa armigera. When mixed with sex pheromone (Ph), some volatiles, e.g. phenyl acetaldehyde, benzyl alcohol, phenylethanol, methylsalicylate, linalool, benzaldehyde, (Z)-3-hexenol, (Z)-3-hexenylacetate, (Z)-6-nonenol, cineole, (E)-2-hexenal, and geraniol elicited stronger responses from male antennae than Ph alone. Wind tunnel bioassay demonstrated that various volatiles could either enhance or inhibit the effect of synthetic sex pheromone. (E)-2-hexenal, (Z)-3-hexenol and linalool in combination with Ph could not induce any male to land on source at all, whereas phenyl acetaldehyde, benzaldehyde, (Z)-6-nonenol and salicylaldehyde combined with Ph enhanced male response rates by 58.63%, 50.33%, 51.85% and 127.78%, respectively, compared to Ph alone. These results suggested that some volatiles shouldmodify sex pheromone caused behavior and that some of them could possibly be used as a tool for disrupting mating or for enhancing the effect of synthetic sex pheromone in the field.

Effects of Soil Fertility and Atmospheric CO_2 Enrichment on Leaf, Stem and Root Dark Respiration of Populus tremuloides

An open-top chamber experiment was conducted at the University of Michigan Biological Station near Pellston, Michigan, USA, to study the effects of soil fertility and CO2 on leaf, stem and root dark respiration (Rd) of Populus tremuloides. Overall, area-based day-time leaf Rd (Rda) was significantly greater at elevated than at ambient CO2 in high-fertility soil, but not in low-fertility soil. Mass-based leaf Rd (Rdm) was overall greater for high- than for low-fertility soil grown trees at elected, but not at ambient CO2. Nighttime leaf Rd. and Rdm were unthected by soil fertility or CO2, nor was stem Rda, which ranged from 1.0 to 1.4 μmol m-2 s-1 in the spring and 3.5 to 4.5 μmol m-2 s-1 in the summer. Root Rda. was significantly higher in high- than in low-fertility soil, but was unaffected by CO2. Since biomass production of P. tremuloides will be significantly greater at elevated CO2 while specific Rd will either increase or remain unchanged, we predict that carbon loss to the atmosphere through respiration from this ecologically important species would increase at higher CO2. Soil fertility would also interact with elevated CO2 in affecting the carbon flow in the plant-soil-air system.

Dynamics of liquid-phase catalytic oxidation of hydrogen sulfide removal in rural biogas

Hydrogen sulfide in rural biogas was removed with liquid-phase catalytic oxidation.By using rare earth as catalyst,and sulfosalicylic acid as stabilizer,H2S purification efficiency could increase as high as 96%,and sulfur capacity of the composite solution was about 3 g/L.The results show that purification efficiency was affected by catalyst addition,pH,experimental temperature,and sulfur capacity.The parameters effects on catalytic oxidation were studied,and the optimized conditions were that Fe3+ concentration 0.08 mg/L,reaction temperature 70°C,pH 9.0,with a absorption solution volume of 50 mL,a gas flow rate 200 mL/min,and H2S mass concentration of 1.58-2.02 mg/m3.

Simultaneous Determination of Six UV-Filters in Cosmetics by HPLC

A reversed-phase high performance liquid chromatographic method with diode-array detector was established to simultaneously determine the six UV-filters, isopentyl-4-methoxycinnamate, ethylhexyl methoxycinnamate, ethylhexyl salicylate, homosalate, butyloctyl salicylate and ethylhexyl triazone. They were separated on a Kromasil C18 column, with gradient elution by methanol and water, and detected by HPLC-DAD.All results demonstrated good linear relationship in proper mass concentration range. The limits of quantitation(LOQ) were between 0.5 and 1.25 mg/L. The recoveries of the six compounds ranged from 98.3% to 101.0% at two inrements with the relative standard deviation(RSD) no more than 1.5%.