[Objective]The study was to analyze the expression of the deletion fragments from the promoter of a glycosyltransferase gene induced both by MeJA and SA cloned from tobacco W38（sm-Ngt） in transgenic tobacco plants.[...[Objective]The study was to analyze the expression of the deletion fragments from the promoter of a glycosyltransferase gene induced both by MeJA and SA cloned from tobacco W38（sm-Ngt） in transgenic tobacco plants.[Method]Using T1 seedlings of sm-Ngt transgenic tobacco lines containing Gus gene controlled by five 5＇ flank deletion promoter fragments different in length as experimental materials,GUS histochemical staining and fluorometric analysis of T1 seedlings treated with MeJA and SA for 16 h were conducted to analyze the effect of MeJA and SA treatment on the expression of 5＇ flank deletion promoter fragments.[Result]Of five 5＇ flank deletion promoter fragments transgenic plant lines,30 d old T1 seedlings containing 220-0 bp promoter fragment performed worst in GUS staining（showing least staining spots）,those containing-524-0 bp and-468-0 bp promoter fragment both performed best.In the plants not treated with MeJA and SA,activities of GUS driven by-524-0 bp and-468-0 bp deletion promoter fragments were enormously higher than that driven by-1 150-0,-800-0 or-220 0 bp,and which were proved to be not resulted from insert copy number by Southern blot.For GUS expression,promoter fragment-800-0 bp expression was doubly induced by both MeJA and SA,while fragment-1 150-0 was induced by MeJA.[Conclusion]There are activity enhancement elements within-524--220 bp of the sm-Ngt in promoter and activity down regulation elements within-1 150--524 bp region,as well as MeJA and SA doubly inducing activity regulation elements in this promoter.展开更多
基金Supported by Natural Science Foundation of Hubei Province(2004ABA123)~~
文摘[Objective]The study was to analyze the expression of the deletion fragments from the promoter of a glycosyltransferase gene induced both by MeJA and SA cloned from tobacco W38（sm-Ngt） in transgenic tobacco plants.[Method]Using T1 seedlings of sm-Ngt transgenic tobacco lines containing Gus gene controlled by five 5＇ flank deletion promoter fragments different in length as experimental materials,GUS histochemical staining and fluorometric analysis of T1 seedlings treated with MeJA and SA for 16 h were conducted to analyze the effect of MeJA and SA treatment on the expression of 5＇ flank deletion promoter fragments.[Result]Of five 5＇ flank deletion promoter fragments transgenic plant lines,30 d old T1 seedlings containing 220-0 bp promoter fragment performed worst in GUS staining（showing least staining spots）,those containing-524-0 bp and-468-0 bp promoter fragment both performed best.In the plants not treated with MeJA and SA,activities of GUS driven by-524-0 bp and-468-0 bp deletion promoter fragments were enormously higher than that driven by-1 150-0,-800-0 or-220 0 bp,and which were proved to be not resulted from insert copy number by Southern blot.For GUS expression,promoter fragment-800-0 bp expression was doubly induced by both MeJA and SA,while fragment-1 150-0 was induced by MeJA.[Conclusion]There are activity enhancement elements within-524--220 bp of the sm-Ngt in promoter and activity down regulation elements within-1 150--524 bp region,as well as MeJA and SA doubly inducing activity regulation elements in this promoter.
