There are many reports about resistance of transgenic Bt poplars to lepidopteran defoliators. Whether the poplars can resist haustellate mouth part pests and leaf-miners remains unclear. This study chose transgenic Bt...There are many reports about resistance of transgenic Bt poplars to lepidopteran defoliators. Whether the poplars can resist haustellate mouth part pests and leaf-miners remains unclear. This study chose transgenic Bt poplar, Populus euramericana cv. Robusta’(R-94), as the material to investigate its resistance to a haustellate mouth part pest, Hegesidemus habrus, and two leaf-miner species, Leucoptera susinella (Lepidoptera: Lyonetiidae) and Lithocolletis paslorella (Lepidoptera: Gracillariidae) in both field and laboratory. Results showed that R-94 poplar had strong resistance to H. habrus in fields. Injury rates of R-94 leaves were respectively only 1.08% in pure stands and 1.1% in mixed stands respectively, while injury rate of 107 poplar (a non-transgenic variety) was 16.3% in the mixed stands. When H. habrus was fed with R-94 leaves in laboratory, the livability of nymphae was 19.9% after 7 days of feeding. However, the livability of nymphae reached to 56.8% after 7 days of feeding with leaves of 107 poplars. When H. habrus was fed with R-94 and 107 poplar leaves together, the insects significantly preferred 107 poplar leaves to R-94 poplar ones. Besides H. habrus, R-94 poplar also showed strong resistance to two leaf-miners species. Therefore, R-94 poplar not only has strong resistance to lepidopteran pest, but also has similar resistance to haustellate mouth part pests and leaf-miners.展开更多
文摘There are many reports about resistance of transgenic Bt poplars to lepidopteran defoliators. Whether the poplars can resist haustellate mouth part pests and leaf-miners remains unclear. This study chose transgenic Bt poplar, Populus euramericana cv. Robusta’(R-94), as the material to investigate its resistance to a haustellate mouth part pest, Hegesidemus habrus, and two leaf-miner species, Leucoptera susinella (Lepidoptera: Lyonetiidae) and Lithocolletis paslorella (Lepidoptera: Gracillariidae) in both field and laboratory. Results showed that R-94 poplar had strong resistance to H. habrus in fields. Injury rates of R-94 leaves were respectively only 1.08% in pure stands and 1.1% in mixed stands respectively, while injury rate of 107 poplar (a non-transgenic variety) was 16.3% in the mixed stands. When H. habrus was fed with R-94 leaves in laboratory, the livability of nymphae was 19.9% after 7 days of feeding. However, the livability of nymphae reached to 56.8% after 7 days of feeding with leaves of 107 poplars. When H. habrus was fed with R-94 and 107 poplar leaves together, the insects significantly preferred 107 poplar leaves to R-94 poplar ones. Besides H. habrus, R-94 poplar also showed strong resistance to two leaf-miners species. Therefore, R-94 poplar not only has strong resistance to lepidopteran pest, but also has similar resistance to haustellate mouth part pests and leaf-miners.
