Structural Analysis of Peptides of PSⅡ Light-harvesting Complexes in Siphonous Green Algae, Codium fragile

Peptide composition and arrangement of 4 major light-harvesting complexes LHCP 1-3 and LHCP 3′ isolated from siphonous green algae (Codium fragile (Sur.) Hariot.) were investigated. LHCP 1 showed five main peptides, 34.4, 31.5, 29.5, 28.2 and 26.5 kD in SDS-PAGE, the 34.4 and 31.5 kD peptides were never found in higher plants. LHCP 3 contained the other four kinds of LHCP 1 peptides except 34.4 kD, while LHCP 3′ consisted of only 28.2 and 26.5 kD peptides. We found that 34.4, 28.2 and 26.5 kD peptides were easy to decompose from LHCP 1 when subjected to SDS-PAGE without pretreatment. They might be located at the exterior of LHCP 1, while the 31.5 and 29.5 kD peptides were at the central part. The 28.2 and 26.5 kD peptides often occurred in CPa, the center complex of PSⅡ. They are possibly the LHCⅡ peptides tightly associated with CCⅡ. According to the results described above, a peptide map of LHCP 1 was sketched.

Effect of forced lamina flow on microsegregation simulated by phase field method quantitatively

The influence of supercooled melt forced lamina flow on microsegregation was investigated. The concentration distribution at solid-liquid boundary of binary alloy Ni-Cu was simulated using phase field model coupled with flow field. The microsegregation, concentration maximum value, boundary thickness of concentration near upstream dendrite and normal to flow dendrite, and downstream dendrite were studied quantitatively in the case of forced lamia flow. The simulation results show that solute field and flow field interact complexly. Compared with melt without flow, in front of upstream dendrite tip, the concentration boundary thickness is the lowest and the concentration maximum value is the smallest for melt with flow. However, in front of downstream dendrite tip, the results are just the opposite. The zone of poor Cu in upstream dendrite where is the most severely microsegregation and shrinkage cavity is wider and the concentration is lower for melt with flow than that without flow.

Vane Shear Strength Based Stability Analysis of Slopes in Unconsolidated Soft Clay

In-situ vane shear test is frequently performed to determine shear strength for slope stability analysis in Tianjin New Harbor.However,the soil shear strength varies with the shear plane orientation.A possible means to reduce the effect of directional dependency of shear strength is to convert the in-situ vane shear strength into undrained shear strength parameters.A method of converting in-situ vane shear strength into undrained shear strength parameters is presented.The shear strength parameters determined for all of the in-situ vane shear strengths are subjected to statistical regression analysis to take into consideration the possible effect of non-homogeneity in the soft clay deposit.Using the regressed shear strength parameters,slope stability analyses are performed for five existing soil slopes.The results of stability analyses indicate that the safety factors obtained from the converted parameters reflect the state of the slopes analyzed much better than those obtained from in-situ vane shear strength and laboratory consolidated-undrained and unconsolidated-undrained strength parameters.It is concluded that the presented methsod of determining undrained shear strength parameters for in-situ vane shear strength is effective.

Superposition model for analyzing the dynamic ground subsidence in mining area of thick loose layer

The dynamic ground subsidence due to underground mining is a complicated time-dependent and rate- dependent process. Based. on the theory of rock rheology and probability integral method, this study developed the superposltlOn model for the prediction and analysis of the ground dynamic subsidence in mining area of thick !oose layer. The model consists of two parts （the prediction of overlying bedrock and the prediction of thick loose layer）. The overlying bedrock is regarded as visco-elastic beam, of which the dynamic subsidence is predicted by the Kelvin visco-elastic rheological model. The thick loose layer is regarded as random medium, and the ground dynamic subsidence, is predicted by the probability integral model. At last, the two prediction models are vertically stacked in the same coordinate system, and the bedrock dynamic subsidence is regarded as a variable mining thickness input into the prediction model of ground dynamic subsidence. The prediction results obtained were compared w^th actual movement and deformation data from Zhao I and Zhao II mine, central China. The agreement of the prediction results with the. field measurements.show that the superposition model （SM） is more satisfactory and the formulae obtained are more effective than the classical single probability Integral model（SPIM）, and thus can be effectively used for predicting the ground dynamic subsidence in mining area of thick loose layer.

Structural elucidation of a new cembranoid diterpene from the Chinese soft coral Sarcophyton sp.

To investigate the cembranoid diterpenes from the soft coral Sarcophyton sp. collected in the South China Sea. Repeatedly column chromatography was performed for the isolation and purification. The structures were elucidated on the basis of extensive spectral data （IR, MS, 1 D- and 2D-NMR） analysis by comparing with literature data. A new cembranoid diterpene namely sarcophyolide A （1）, together with 7a, 8β-dihydroxydeepoxysarcophine （2） were isolated from this species. The cembranoid diterpenes with 7-1actone could be chemotaxonomic markers of Sarcophyton sp.

Pyrolysis and combustion kinetics of lycopodium particles in thermogravimetric analysis

Biomass is a kind of renewable energy which is used increasingly in different types of combustion systems or in the production of fuels like bio-oil. Lycopodium is a cellulosic particle, with good combustion properties, of which microscopic images show that these particles have spherical shapes with identical diameters of 31 μm. The measured density of these particles is 1.0779 g/cm2. Lycopodium particles contain 64.06% carbon, 25.56% oxygen, 8.55% hydrogen and 1.83% nitrogen, and no sulfur. Thermogravimetric analysis in the nitrogen environment indicates that the maximum of particle mass reduction occurs in the temperature range of 250-550 ℃ where the maximum mass reduction in the DTG diagrams also occurs in. In the oxygen environment, an additional peak can also be observed in the temperature range of 500-600 ℃, which points to solid phase combustion and ignition temperature of lycopodium particles. The kinetics of reactions is determined by curve fitting and minimization of error.

Design and Experimental Analysis of NLT Trussed Rafter in Yellow Pine

The roof system of social housing in Brazil generally consists of components made out of native forest lumber of high market value. Taking into account the increasing number of planted forests and the need to develop new products and to add value to this timber, this work deals with the development and structural analysis of a roof system using yellow pine plantation wood （Pinus spp）, a sustainable material which however presents many defects. The NLT （laminated nailed timber） technology was chosen as it allows the use of shorter length and smaller cross section pieces, eliminating major defects. Seven samples of structural trussed rafters in NLT were tested; six made out of graded timber and one ungraded in order to verify the impact of the wood grading in the structural performance of the model. The results showed that the trussed rafter system in NLT meets the necessary structural performance requiring poor conditions of infrastructure for manufacture process, and that the graded wood samples showed better performance than the ungraded one.

Analysis on probability of water inrush and quicksand in different mining sequences under an unconsolidated alluvium aquifer by fluid-solid coupling theory

To study the behavior of overlying strata and the likelihood of water inrush and quicksand with different mining sequences under an unconsolidated alluvium aquifer, a numerical model based on the fluid-solid coupling theory was con- structed by FLAC3D. Simulation results revealed that the mining sequences had a significant influence on the seepage, dis- placement and failure characteristics of the overlying strata. In this kind of geological and hydrogeological conditions, the workface close to the outcrop of coal seam easily suffers from water inrush and quicksand during mining. In the simulation re- sults, the plastic zone, vertical displacement and pore water pressure in the overlying strata of the workface decrease more or less using the upward mining sequence than using the downward mining sequence. Therefore, the application of the upward mining sequence in the process of mining is preferential to prevent water inrush and quicksand.

Identification of differentially expressed long non-coding RNAs in human ovarian cancer cells with different metastatic potentials

Objective： To identify differentially expressed long non-coding RNAs （lncRNAs） involved in the metastasis of epithelial ovarian cancer. Methods： An in vitro invasion assay was performed to validate the invasive capability of SKOV3 and SKOV3.ip1 cell lines. Total R.NA was then extracted, and microarray analysis was performed. Moreover, nine lncRNAs were selected for validation using RT-qPCR. Results： Compared with the SKOV3 cells, the SKOV3.ip1 cells significantly improved in the in vitro invasive activity. Of the 4,956 lncRNAs detected in the microarra~ 583 and 578 lncRNAs were upregulated and downregulated, respectivel~ in SKOV3.ip1 cells, compared with the parental SKOV3 cells. Seven of the analyzed lncRNAs （MALAT1, H19, UCA1, CCAT1, LOC645249, LOC100128881, and LOC100292680） confirmed the deregulation found by microarray analysis. Conclusion： LncRNAs clusters were differentially expressed in ovarian cancer cells with varying metastatic potentials. This result indicates that some lncRNAs might exert a partial or key role in epithelial ovarian cancer metastasis. Further studies should be conducted to determine the roles of these lncRNAs in ovarian cancer metastasis.

Fractal analysis of thermal conduction of loose coal

For deep mining engineering, heat transfer of coal mass is a vital factor in the thermal environment of coal mines. In order to study the thermal conduction mechanism, we obtained gray images of coal mass microstructure by scanning samples with a digital microscope. With the use of Matlab, these gray images were transformed into binary images, which were then transformed into a corresponding matrix consisting only of the values 0 and 1. According to the calculation method of box-counting dimension, we calculated the fractal dimension of the loose coal to be approximately 1.86. The thermal conductivity expressions of loose coal were derived based on the simulation method of thermal resistance. We calculated the thermal conductivity of loose coal by using a fractal model and compared the calculated values with our experimental data. The results show that the test data show an encourag-ing agreement with the calculated values. Hence fractal theory is a feasible method for studying thermal conductivity of loose coal.

Singular Hybrid Boundary Node Method for Solving Poisson Equation

As a boundary-type meshless method, the singular hybrid boundary node method（SHBNM） is based on the modified variational principle and the moving least square（MLS） approximation, so it has the advantages of both boundary element method（BEM） and meshless method. In this paper, the dual reciprocity method（DRM） is combined with SHBNM to solve Poisson equation in which the solution is divided into particular solution and general solution. The general solution is achieved by means of SHBNM, and the particular solution is approximated by using the radial basis function（RBF）. Only randomly distributed nodes on the bounding surface of the domain are required and it doesn＇t need extra equations to compute internal parameters in the domain. The postprocess is very simple. Numerical examples for the solution of Poisson equation show that high convergence rates and high accuracy with a small node number are achievable.

WentzeI-Freidlin Estimates for Jump Processes in Semi-Group Theory： Upper Bound

Large deviations estimates for Poisson processes estimate the logarithm of rare events associated to a Poisson process which has more and more jump which are smaller and smaller. In stochastic analysis, they are valid on the whole path space. Asoociated to this jump process is a Markov semi-group. We translate in semi group theory the proof of Wentzel-Freidlin for these estimates by translating in semi-group theory some basical tools of stochastic analysis as the exponential martingales of stochastic analysis. These Wentzel-Freidlin estimates （upper-bound） are only true for the semi-group.

Numerical analysis on car-following traffic flow models with delay time

Effects of the speed relaxation time on the optimal velocity car-following model (OVM) with delay time due to driver reaction time proposed by Bando et al.(1995) were studied by numerical methods. Results showed that the OVM including the delay is not physically sensitive to the speed relaxation times. A modified car-following model is proposed to overcome the deficiency. Analyses of the linear stability of the modified model were conducted. It is shown that coexisting flows appear if the initial homogeneous headway of the traffic flow is between critical values. In addition, phase transitions occur on varying the initially homogeneous headway.

INFLUENCE OF UNCERTAINTY OF ROCK PROPERTIES ON SEISMIC RESPONSES OF REACTOR BUILDINGS

The influence of the dispersion and uncertainty of the dynamic shear wave velocity and Poisson＇s ratio of soil in a hard rock site was investigated on the seismic response of reactor building structure. The analysis is performed by considering the soil-structure interaction effects and based on the model of the reactor building in a typical pressurized water reactor nuclear power plant （NPP）. The numerical results show that for the typical floor selected, while the relative increment ratio of the dynamic shear wave velocity varies from -30% to 30% compared to the basis of 1 930 m/s, the relative variation of the horizontal response spectra peak value lies in the scope of ±10% for the internal structure, and the relative variation of the frequency corresponding to the spectra peak is 0.0% in most cases. The relative variation of the vertical response spectra peak value lies in the scope of - 10% to 22%, and the relative variation of the frequency corresponding to the Spectra peak lies in the scope of - 22% to 4%. The analysis indicates that the dynamic shear wave velocity and the Poisson＇s ratio of the rock would affect the seismic response of structure and the soil-structure interaction effects should be considered in seismic analysis and design of NPP even for a hard rock site.

Quantitative Analysis of Relationships Between Crack Characteristics and Properties of Soda-saline Soils in Songnen Plain, China

The Songnen Plain has a typical soda-saline soil, which often shrinks and cracks under natural conditions during water evaporation. This study aims to analyze the relationships between the crack characteristics and the soil properties of soda-saline soils quantitatively, and attempts to establish prediction models for the main soil properties of soda-saline soils based on the results. In order to achieve these objectives, a desiccation cracking test was conducted using 17 soil specimens with different salinity levels under controlled laboratory conditions. Correlation analysis was then performed between the crack characteristics and the soil properties. The results indicate that the crack characteristics can well represent the surface appearances of cracked soils, they also can well distinguish the salinity levels of soda-saline soils while the clay contents and mineralogical compositions of soils are stable. Among the crack characteristics, crack length has the best relationships with the salinity levels of soda-saline soils. Specifically, the crack length has high correlation(R2 > 0.87) with the electrical conductivity(EC), Na+, CO32– and the salinity, it also has reasonable relationship(R2 > 0.68) with HCO3–, this indicates crack length can be well used for the prediction of these properties of soda-saline soils.

Analysis of Efficacy of DICE(Dexamethasone,Ifosfamide,Cisplatin and Etoposide)Regimen on Recurrent and Refractory Intermediate and High Grade Non-Hodgkin's Lymphoma

OBJECTIVE Thus far there is no standard salvage regimenfor patients with recurrent and refractory intermediate and highgrade non-Hodgkin's lymphoma (NHL). This study intends toinvestigate the therapeutic efficacy of the DICE (dexamethasone,isofosfamide, cisplatin and etoposide) regimen on the recurrentand refractory NHL, and to observe the related adverse effects.METHODS Clinical records of 22 patients with recurrent andrefractory NHL, who failed to achieve a remission from theCHOP [cyclophosphamide, hydroxydaunomycin/doxorubicin(adriamycin), oncovin, prednisone] regimen within 2 to 6 cyclesof treatment, were reviewed. DICE, as a salvage regimen with amedian course of treatment of 4 cycles (ranging from 2 to 7 cycles),was now used, and evaluation of the therapeutic efficacy andadverse effect of DICE was conducted in all the patients. Of the 22NHL cases, 8 were of T-cell origin and the other 14 B-cell origin.Salvage treatment was performed in the patients, with appraisal,prevention and treatment of the toxic reactions.RESULTS Following DICE treatment in the 22 patients, thetotal effective rate of the regimen was 63.6%, and the completeremission (CR) rate was 40.9%. The effective rates of DICE onthe T and B-cell sourced NHL were 75.0% and 57.1%, and the CRrate were 37.5%, 42.9%, respectively (P >0.05). An increase of thelactate dehydrogenase (LDH) level accompanied by a giant lumpwas the short-term effect on patients with recurrence (mean P <0.05) who were drug resistant. Myelosuppression, digestive systemreaction and alopecia were the commonly-seen complications inthe patients who received DICE regimen. All patients recoveredafter treatment, and no chemotherapy-related death occurred.CONCLUSION DICE regimen is effective in treating refractoryand recurrent NHL.

Research on the relationship between financial slack and R ＆ D investment in manufacturing industry under dynamic environment

As a part of the redundancy of organization, financial slack has become the focus of academic research. This paper takes the listed companies of manufacturing industry above Designated Size as sample, using multiple regressionto examine the relationship between corporate financial slack and R ＆ D investment under dynamic environment, using the Malmquist index method based on DEA model to analyze the innovation efficiency of Chinese manufacturing industry during the period of 2009-2013. The results show that： Financial slack and R ＆ D investment intensity presents inverted ＂U＂ type relationship in？sample？companies, and the relationship is positively regulated by environmental dynamism; Financial slackpromotes R ＆ D investment but have lag effect.

Research on the impact of financial slack to product competition ——Based on the growth perspective of real estate enterprises

This article is based on growth, financial multidimensional perspective of redundancy and product market competition, focus on growth as a key variable, to explore the growth of financial redundancy and product market competition. The data of 326 real estate companies were selected for empirical analysis. The research finds that the financial redundancy promotes competition in the product market, and the relationship between the growth of the enterprise growth and the competition between the financial redundancy and the competition of the product market.

Petrological evidence of kaolinite transformation to dawsonite

Clay minerals, especially authigene kaolinite, are abundant in the dawsonite-bearing sandstone from the Gudian and Qian＇an regions in the southern Songliao Basin. The relationship between dawsonite and kaolinite has been investigated based on drilling data from 50 wells in the Jilin Oil Field and petrological cbaracteristics analyses on 23 core samples from the Gudian and Qian＇an regions. The study revealed a negative relationship between the content of dawsonite and kaolinite, suggesting that kaolinite was replaced by the dawsonite. Combined with thermodynamics and geochemical analysis published previously, it is concluded that kaolinite is unstable under CO2-rich environments and can transform into dawsonite.