松江鲈承载着深厚的历史文化,称赞其资料有古代时期的书籍、诗人描写的诗词、以及名人与它有关的故事。后来在文学作品中以“莼鲈之思”作为思乡之情的代名词,至今仍广为流传和引用。文化是政治与经济的反应,历代文人纷纷在诗词歌赋中...松江鲈承载着深厚的历史文化,称赞其资料有古代时期的书籍、诗人描写的诗词、以及名人与它有关的故事。后来在文学作品中以“莼鲈之思”作为思乡之情的代名词,至今仍广为流传和引用。文化是政治与经济的反应,历代文人纷纷在诗词歌赋中描写到松江鲈,也反映出了当时社会的风貌。因此,本文在大量收集史料的基础上,以时间为顺序探究松江鲈文化,对松江鲈古代文化以朝代发展为脉络,分为“萌芽”、“兴起”、“鼎盛”、“没落”四个阶段对松江鲈的诗词歌赋进行概述,为查阅松江鲈古代文化提供科学资料,同时也为渔业文化行业注入新鲜血液,以便人们在深入了解松江鲈文化的基础上,更好地认识松江鲈这一珍贵物种资源,崇尚松江鲈鱼文化。Songjiang Perch carries a profound historical culture, and its praise materials including ancient books, poems written by poets, and stories related to it by celebrities. Later, he used “Chun Lu Zhi Si” as a synonym for homesickness in his literary works, which is still widely spread and quoted today. Culture is a reflection of politics and economy. The writers of all dynasties have described Songjiang perch in their poems and poems, which also reflects the social style at that time. Therefore, in this paper, on the basis of a large collection of historical data, in time for the order to explore the Songjiang perch culture, to Songjiang perch for ancient culture with dynasty development context, divided into “green shoots”, “rise”, “golden”, “fall” four stages of Songjiang perch verses are summarized, to provide scientific data for refer to Songjiang perch ancient culture, but also inject fresh blood for the fisheries culture industry. In order to better understand Songjiang perch, a precious species resource, and advocate Songjiang perch culture on the basis of in-depth understanding of Songjiang perch culture.展开更多
The mechanisms underlying sex determination and differentiation have long intrigued researchers in the fields of development and evolutionary biology.The roughskin sculpin(Trachidermus fasciatus Heckel),displaying sex...The mechanisms underlying sex determination and differentiation have long intrigued researchers in the fields of development and evolutionary biology.The roughskin sculpin(Trachidermus fasciatus Heckel),displaying sexual dimorphism,provides an ideal model for studying the mechanisms.However,both genetic and genomic information concerning sex determination and differentiation,such as gonadal transcriptome data in roughskin sculpin,are lacking.Here,we present the first gonadal transcriptomes of roughskin sculpin and identify sex-related genes.We identified 8531 differentially expressed genes(DEGs),among them 4065 were upregulated in the ovary and 4466 upregulated in the testis.Several sex-related gene ontology(GO)terms were enriched in ovary-biased genes,including“binding of sperm to zona pellucida”,“egg coat formation”,“positive regulation of acrosome reaction”,“cell division”,and“cell cycle”,while the GO terms such as“spermatogenesis”,“sperm axoneme assembly”,“cilium assembly”,“cilium movement”,and“cilium movement involved in cell motility”were enriched in testis-biased genes.Moreover,six KEGG pathways were significantly enriched in the ovary,whereas only one was enriched in the testis.Of these DEGs,40 sex-related genes were identified,which including 26 testis-biased genes(such as Dmrtb 1,Gsdf,Sox 9 b,Wnt 4 b,Tcp 11 l 2,and Efhb),and 14 ovary-biased genes(such as Cyp 19 a 1 a,Foxh 1,Foxr 1,Gdf 3,Hsd 17 b 12,and Igf 2 bp 3).This gonadal transcript dataset would broaden our understanding of sex determination and differentiation mechanisms in roughskin sculpin,expand the genomic database,support future studies on sex-related gene functions,and facilitate molecular biology research into roughskin sculpin.展开更多
[Objective] The aim of this study is to establish and optimize the ISSR-PCR system in roughskin sculpin(Trachidermus fasciatus Heckel).[Method] By using the primer of ISSR-63,the concentrations of Taq DNA polymerase,M...[Objective] The aim of this study is to establish and optimize the ISSR-PCR system in roughskin sculpin(Trachidermus fasciatus Heckel).[Method] By using the primer of ISSR-63,the concentrations of Taq DNA polymerase,Mg2+,dNTPs and primer were optimized by orthogonal design for establishing the suitable ISSR-PCR system in roughskin sculpin;moreover,the suitable anneal temperature was yielded from gradient PCR on temperature.[Result]The optimized ISSR-PCR system(20 μl reaction volume)in roughskin sculpin was proved to be:2.5 mmol/L Mg2+,250 μmol/L dNTPs,0.25 μmol/L primer,1 U Taq DNA polymerase,30 ng DNA template and 1×PCR buffer;and suitable anneal temperature was determined to be 50.8 ℃.The established system was further confirmed by using 24 wild roughskin sculpin samples.[Conclusion]The results lay a foundation for the analysis of genetic diversity and germplasm resources of roughskin sculpin.展开更多
文摘松江鲈承载着深厚的历史文化,称赞其资料有古代时期的书籍、诗人描写的诗词、以及名人与它有关的故事。后来在文学作品中以“莼鲈之思”作为思乡之情的代名词,至今仍广为流传和引用。文化是政治与经济的反应,历代文人纷纷在诗词歌赋中描写到松江鲈,也反映出了当时社会的风貌。因此,本文在大量收集史料的基础上,以时间为顺序探究松江鲈文化,对松江鲈古代文化以朝代发展为脉络,分为“萌芽”、“兴起”、“鼎盛”、“没落”四个阶段对松江鲈的诗词歌赋进行概述,为查阅松江鲈古代文化提供科学资料,同时也为渔业文化行业注入新鲜血液,以便人们在深入了解松江鲈文化的基础上,更好地认识松江鲈这一珍贵物种资源,崇尚松江鲈鱼文化。Songjiang Perch carries a profound historical culture, and its praise materials including ancient books, poems written by poets, and stories related to it by celebrities. Later, he used “Chun Lu Zhi Si” as a synonym for homesickness in his literary works, which is still widely spread and quoted today. Culture is a reflection of politics and economy. The writers of all dynasties have described Songjiang perch in their poems and poems, which also reflects the social style at that time. Therefore, in this paper, on the basis of a large collection of historical data, in time for the order to explore the Songjiang perch culture, to Songjiang perch for ancient culture with dynasty development context, divided into “green shoots”, “rise”, “golden”, “fall” four stages of Songjiang perch verses are summarized, to provide scientific data for refer to Songjiang perch ancient culture, but also inject fresh blood for the fisheries culture industry. In order to better understand Songjiang perch, a precious species resource, and advocate Songjiang perch culture on the basis of in-depth understanding of Songjiang perch culture.
基金Supported by the National Natural Science Foundation of China(Nos.31972793,31502169)the Key Scientific Research Project in Universities and Colleges in Tianjin(No.2022ZD004)。
文摘The mechanisms underlying sex determination and differentiation have long intrigued researchers in the fields of development and evolutionary biology.The roughskin sculpin(Trachidermus fasciatus Heckel),displaying sexual dimorphism,provides an ideal model for studying the mechanisms.However,both genetic and genomic information concerning sex determination and differentiation,such as gonadal transcriptome data in roughskin sculpin,are lacking.Here,we present the first gonadal transcriptomes of roughskin sculpin and identify sex-related genes.We identified 8531 differentially expressed genes(DEGs),among them 4065 were upregulated in the ovary and 4466 upregulated in the testis.Several sex-related gene ontology(GO)terms were enriched in ovary-biased genes,including“binding of sperm to zona pellucida”,“egg coat formation”,“positive regulation of acrosome reaction”,“cell division”,and“cell cycle”,while the GO terms such as“spermatogenesis”,“sperm axoneme assembly”,“cilium assembly”,“cilium movement”,and“cilium movement involved in cell motility”were enriched in testis-biased genes.Moreover,six KEGG pathways were significantly enriched in the ovary,whereas only one was enriched in the testis.Of these DEGs,40 sex-related genes were identified,which including 26 testis-biased genes(such as Dmrtb 1,Gsdf,Sox 9 b,Wnt 4 b,Tcp 11 l 2,and Efhb),and 14 ovary-biased genes(such as Cyp 19 a 1 a,Foxh 1,Foxr 1,Gdf 3,Hsd 17 b 12,and Igf 2 bp 3).This gonadal transcript dataset would broaden our understanding of sex determination and differentiation mechanisms in roughskin sculpin,expand the genomic database,support future studies on sex-related gene functions,and facilitate molecular biology research into roughskin sculpin.
基金Supported by High Tech Project from Sci-tech Department of Jiangsu Province(BG2006337)~~
文摘[Objective] The aim of this study is to establish and optimize the ISSR-PCR system in roughskin sculpin(Trachidermus fasciatus Heckel).[Method] By using the primer of ISSR-63,the concentrations of Taq DNA polymerase,Mg2+,dNTPs and primer were optimized by orthogonal design for establishing the suitable ISSR-PCR system in roughskin sculpin;moreover,the suitable anneal temperature was yielded from gradient PCR on temperature.[Result]The optimized ISSR-PCR system(20 μl reaction volume)in roughskin sculpin was proved to be:2.5 mmol/L Mg2+,250 μmol/L dNTPs,0.25 μmol/L primer,1 U Taq DNA polymerase,30 ng DNA template and 1×PCR buffer;and suitable anneal temperature was determined to be 50.8 ℃.The established system was further confirmed by using 24 wild roughskin sculpin samples.[Conclusion]The results lay a foundation for the analysis of genetic diversity and germplasm resources of roughskin sculpin.
