[Objective] This study was aimed to review the controlling experience of pine wilt disease in the past 25 years, explore the theories and methods of controlling pine wilt disease, and improve the scientific level of c...[Objective] This study was aimed to review the controlling experience of pine wilt disease in the past 25 years, explore the theories and methods of controlling pine wilt disease, and improve the scientific level of controlling techniques and the protection capacity of healthy pine trees. [Method] Eleven items of effects were used to refine the theory of clearing dead pine trees affected by pine wilt disease, namely, "1 priority", "2 objections", "3 principles", "4 measures", and "5 manage- ments". On the basis of comprehensive control and complete removal of the infect- ed pine trees, a variety of comprehensive and efficient controlling methods were developed to carry out targeted chemical ecology trapping, bionic pesticide killing and releasing natural enemies of Sclerodermus guani, Dastarcus helophoroides. High ef- ficient emamectin benzoate immune injection was developed to inject the healthy pine trees for prevention, so as to extinguish the pine wilt disease. [Result] The pine wilt disease dropped from the peak of 3.5 million dead trees with an infecting area of 28 273 hectares in 1999 to 0.068 million with an area of 4 333 hectares in 2012 gradually, reducing by 98.06% in number and 84.84% in area, respectively. On the basis of removal, Dastarcus helophoroides was also released, which could make the number of dead pines decrease more significantly than the control, and af- ter releasing for 5 consecutive years, the dead pine trees dropped to 0.511 plant/hm2 in 2012, with a mortality rate of 0.022 7%, which achieved the control effect, reaching extremely significant level. "Forest land removal+infected trees isolation+natural enemy release" could extinguish the pine wilt disease. The test of isolating 24 heaps of infected pine trees showed that there were 9 heaps of pine trees extinguished the pine wilt disease, which controlled the occurrence of pine wilt disease for 100%, accounting for 37.5% of the total, in which the number of those isolated using iron netting and nylon net were 4 for each, accounting for 88.9%, and there was one heap using polypropylene net, accounting for 11.1%. The invention of em- amectin benzoate immune injection laid the foundation for extinguishing pine wilt disease. The follow checking of the effects of emamectin benzoate immune injection on pine wilt disease found that the number of dead trees caused by pine wilt dis- ease decreased significantly after injecting, and became very small in October of the next year, and the disease was completely extinguished in the third year. [Conclusionl Pine wilt disease could be controlled and extinguished with positive control by using "comprehensive cleaning+industrialized removal", "comprehensive cleaning+ natural enemy release", "comprehensive cleaning+infected trees isolation+natural ene- my release" and "comprehensive cleaning+emamectin benzoate immune".展开更多
The paper separately discusses interplay of pine wood nematode and several key biological factors related to pine wilt disease, such as host trees, insect vectors, fungi, symbiotic bacteria, and natural enemy acarid, ...The paper separately discusses interplay of pine wood nematode and several key biological factors related to pine wilt disease, such as host trees, insect vectors, fungi, symbiotic bacteria, and natural enemy acarid, etc. By virtue of so much complicated interplay among the biological factors related to pine wilt disease which is different from other forest disease, it also points out that the further researches about pine wilt disease should focus on interplay mechanism of key biological factors to discover pathogenic mechanism, and simple and quick inspection and quarantine methods.展开更多
This article briefly introduced the disease incidence and disservice situation in China. The disease is regarded as cancer in pines for its higher spread and death speed and the difficulty in prevention. Strict quaran...This article briefly introduced the disease incidence and disservice situation in China. The disease is regarded as cancer in pines for its higher spread and death speed and the difficulty in prevention. Strict quarantine is the main measure on countrol of the disease, for there are many difficulties in the prevention. The most important part of the disease study is the early diagnosis and detection.展开更多
基金Supported by the Scientific Research Project of National Level of YANG Zhongqi of Chinese Academy of Forestry(2012AA101503)~~
文摘[Objective] This study was aimed to review the controlling experience of pine wilt disease in the past 25 years, explore the theories and methods of controlling pine wilt disease, and improve the scientific level of controlling techniques and the protection capacity of healthy pine trees. [Method] Eleven items of effects were used to refine the theory of clearing dead pine trees affected by pine wilt disease, namely, "1 priority", "2 objections", "3 principles", "4 measures", and "5 manage- ments". On the basis of comprehensive control and complete removal of the infect- ed pine trees, a variety of comprehensive and efficient controlling methods were developed to carry out targeted chemical ecology trapping, bionic pesticide killing and releasing natural enemies of Sclerodermus guani, Dastarcus helophoroides. High ef- ficient emamectin benzoate immune injection was developed to inject the healthy pine trees for prevention, so as to extinguish the pine wilt disease. [Result] The pine wilt disease dropped from the peak of 3.5 million dead trees with an infecting area of 28 273 hectares in 1999 to 0.068 million with an area of 4 333 hectares in 2012 gradually, reducing by 98.06% in number and 84.84% in area, respectively. On the basis of removal, Dastarcus helophoroides was also released, which could make the number of dead pines decrease more significantly than the control, and af- ter releasing for 5 consecutive years, the dead pine trees dropped to 0.511 plant/hm2 in 2012, with a mortality rate of 0.022 7%, which achieved the control effect, reaching extremely significant level. "Forest land removal+infected trees isolation+natural enemy release" could extinguish the pine wilt disease. The test of isolating 24 heaps of infected pine trees showed that there were 9 heaps of pine trees extinguished the pine wilt disease, which controlled the occurrence of pine wilt disease for 100%, accounting for 37.5% of the total, in which the number of those isolated using iron netting and nylon net were 4 for each, accounting for 88.9%, and there was one heap using polypropylene net, accounting for 11.1%. The invention of em- amectin benzoate immune injection laid the foundation for extinguishing pine wilt disease. The follow checking of the effects of emamectin benzoate immune injection on pine wilt disease found that the number of dead trees caused by pine wilt dis- ease decreased significantly after injecting, and became very small in October of the next year, and the disease was completely extinguished in the third year. [Conclusionl Pine wilt disease could be controlled and extinguished with positive control by using "comprehensive cleaning+industrialized removal", "comprehensive cleaning+ natural enemy release", "comprehensive cleaning+infected trees isolation+natural ene- my release" and "comprehensive cleaning+emamectin benzoate immune".
基金The paper is supported by National Basic Research and Development Program, 2002CB111404.
文摘The paper separately discusses interplay of pine wood nematode and several key biological factors related to pine wilt disease, such as host trees, insect vectors, fungi, symbiotic bacteria, and natural enemy acarid, etc. By virtue of so much complicated interplay among the biological factors related to pine wilt disease which is different from other forest disease, it also points out that the further researches about pine wilt disease should focus on interplay mechanism of key biological factors to discover pathogenic mechanism, and simple and quick inspection and quarantine methods.
文摘This article briefly introduced the disease incidence and disservice situation in China. The disease is regarded as cancer in pines for its higher spread and death speed and the difficulty in prevention. Strict quarantine is the main measure on countrol of the disease, for there are many difficulties in the prevention. The most important part of the disease study is the early diagnosis and detection.