Antioxidant and antimicrobial activities of Echinacea purpurea L. (Asteraceae) extracts obtained by classical and ultrasound solvent extraction were compared. The dry aerial part of plant was extracted by 70% etha-nol...Antioxidant and antimicrobial activities of Echinacea purpurea L. (Asteraceae) extracts obtained by classical and ultrasound solvent extraction were compared. The dry aerial part of plant was extracted by 70% etha-nol at a solid-to-liquid ratio of 1︰10 (m/v) and 25°C. The extract obtained by classical solvent extraction contained 29% larger amounts of phenolic compounds and 20% higher content of flavonoids. 2,2-diphenyl-1-picril hydrazyl radical (DPPH) scavenging reached 93.6% and the values of EC50 were (34.16±0.65) μg·ml-1 and (65.48±1.12) μg·ml-1 for the extracts obtained by the classical and ultrasound extractions, respectively. The extracts, independent of the extraction technique applied, showed a considerable growth inhibition on Candida albicans and Saccharo-myces cerevisiae, while no growth inhibition zones were observed for Aspergillus niger. The diameters of inhibition zone observed for all the microorganisms were larger for extracts obtained by classical extraction than those by ul-trasound extraction.展开更多
Echinacoside is one of the phenylethanoids isolated from the stems of Cistanches salsa. Our previous research showed that echinacoside has anti-senescence activity. To investigate the mechanism of echinacoside＇s anti...Echinacoside is one of the phenylethanoids isolated from the stems of Cistanches salsa. Our previous research showed that echinacoside has anti-senescence activity. To investigate the mechanism of echinacoside＇s anti-senescence activity, the expressions of p53, p21, p16 and Rb at the mRNA and protein levels were determined. Results showed that the expression of p53 was down-regulated significantly in a dose dependent manner after treatment with echinacoside. Further experiments suggested that the down-regulation of p53 may be correlated with the upregulation of SIRT1. In addition, echinacoside may exhibit considerable higher affinity towards SIRT1 than resveratrol, according to our molecular docking simulation. In conclusion, we expect that echinacoside might be a promising candidate for regulating cell senescence.展开更多
目的:探讨松果菊苷诱导大鼠骨髓间充质干细胞向成骨细胞分化的作用及可能的机制。方法:MTT实验观察不同浓度(7.867×10~3mg/L,78.67mg/L,0.7867mg/L,7.867×10^(-3)mg/L)松果菊苷对细胞增殖的影响,采用ELISA测定不同浓度松果菊...目的:探讨松果菊苷诱导大鼠骨髓间充质干细胞向成骨细胞分化的作用及可能的机制。方法:MTT实验观察不同浓度(7.867×10~3mg/L,78.67mg/L,0.7867mg/L,7.867×10^(-3)mg/L)松果菊苷对细胞增殖的影响,采用ELISA测定不同浓度松果菊苷组的碱性磷酸酶(ALP)和骨钙素(BGP)的表达,评价成骨分化的能力。免疫荧光和Western blot检测BMP2、Smad1、Runx2蛋白的表达,RT-PCR检测BMP2、Smad1、Runx2 m RNA的表达,分析成骨分化的可能作用机制。结果:MTT实验结果显示78.67mg/L和0.7867mg/L的松果菊苷促进细胞增殖的能力较好。经典成骨诱导组(10%FBS+3.061×103mg/Lβ-甘油磷酸钠+3.925×10-3mg/L地塞米松+8.806mg/L维生素C)和78.67mg/L的松果菊苷组的ALP(48.07)和BGP(141.67)表达均明显增强。经典成骨诱导组和松果菊苷组的BMP2、Smad1、Runx2蛋白表达及BMP2、Smad1、Runx2 m RNA表达明显增强。结论:松果菊苷可以促进体外培养的骨髓间充质干细胞向成骨细胞分化,其作用机制与BMP2、Smad1、Runx2表达增加有关。展开更多
基金Supported by the Ministry of Science and Environmental Protection,Republic of Serbia (142073B)
文摘Antioxidant and antimicrobial activities of Echinacea purpurea L. (Asteraceae) extracts obtained by classical and ultrasound solvent extraction were compared. The dry aerial part of plant was extracted by 70% etha-nol at a solid-to-liquid ratio of 1︰10 (m/v) and 25°C. The extract obtained by classical solvent extraction contained 29% larger amounts of phenolic compounds and 20% higher content of flavonoids. 2,2-diphenyl-1-picril hydrazyl radical (DPPH) scavenging reached 93.6% and the values of EC50 were (34.16±0.65) μg·ml-1 and (65.48±1.12) μg·ml-1 for the extracts obtained by the classical and ultrasound extractions, respectively. The extracts, independent of the extraction technique applied, showed a considerable growth inhibition on Candida albicans and Saccharo-myces cerevisiae, while no growth inhibition zones were observed for Aspergillus niger. The diameters of inhibition zone observed for all the microorganisms were larger for extracts obtained by classical extraction than those by ul-trasound extraction.
基金National Basic Research Program(973 Project) of China(Grant No.2007CB507406)the National Natural Science Foundation of China(Grant No.30572341)the Tsinghua-Yue-Yuen Medical Sciences Fund(THYY20070008)
文摘Echinacoside is one of the phenylethanoids isolated from the stems of Cistanches salsa. Our previous research showed that echinacoside has anti-senescence activity. To investigate the mechanism of echinacoside＇s anti-senescence activity, the expressions of p53, p21, p16 and Rb at the mRNA and protein levels were determined. Results showed that the expression of p53 was down-regulated significantly in a dose dependent manner after treatment with echinacoside. Further experiments suggested that the down-regulation of p53 may be correlated with the upregulation of SIRT1. In addition, echinacoside may exhibit considerable higher affinity towards SIRT1 than resveratrol, according to our molecular docking simulation. In conclusion, we expect that echinacoside might be a promising candidate for regulating cell senescence.
文摘目的:探讨松果菊苷诱导大鼠骨髓间充质干细胞向成骨细胞分化的作用及可能的机制。方法:MTT实验观察不同浓度(7.867×10~3mg/L,78.67mg/L,0.7867mg/L,7.867×10^(-3)mg/L)松果菊苷对细胞增殖的影响,采用ELISA测定不同浓度松果菊苷组的碱性磷酸酶(ALP)和骨钙素(BGP)的表达,评价成骨分化的能力。免疫荧光和Western blot检测BMP2、Smad1、Runx2蛋白的表达,RT-PCR检测BMP2、Smad1、Runx2 m RNA的表达,分析成骨分化的可能作用机制。结果:MTT实验结果显示78.67mg/L和0.7867mg/L的松果菊苷促进细胞增殖的能力较好。经典成骨诱导组(10%FBS+3.061×103mg/Lβ-甘油磷酸钠+3.925×10-3mg/L地塞米松+8.806mg/L维生素C)和78.67mg/L的松果菊苷组的ALP(48.07)和BGP(141.67)表达均明显增强。经典成骨诱导组和松果菊苷组的BMP2、Smad1、Runx2蛋白表达及BMP2、Smad1、Runx2 m RNA表达明显增强。结论:松果菊苷可以促进体外培养的骨髓间充质干细胞向成骨细胞分化,其作用机制与BMP2、Smad1、Runx2表达增加有关。
