A simple and green technique has been developed to prepare hierarchical biomorphic ZrO2- CeO2, using silkworm silk as the template. Different from traditional immersion technics, the whole synthesis process depends mo...A simple and green technique has been developed to prepare hierarchical biomorphic ZrO2- CeO2, using silkworm silk as the template. Different from traditional immersion technics, the whole synthesis process depends more on the restriction or direction functions of the silkworm silk template. The analytic results showed that ZrO2-CeO2 exhibited a well-crystallized hierarchically interwoven hollow fiber structure with 16-28 μm in diameter. The grain size of the sample calcined at 800 ℃ was about 14 nm. Consequently, the interwoven meshwork at three dimensions is formed due to the direction of biotemplate. The action mechanism is summarily discussed here. It may bring the biomorphic ZrO2-CeO2 nanomaterials with hierarchical interwoven structures to more applications, such as catalysts.展开更多
This paper advances a new concept of textile-meltglue cloth, and introduces the readers to the basic principle of melt-glue cloth. On the basis of melt spinning, the spinneret can be replaced by a spinning device that...This paper advances a new concept of textile-meltglue cloth, and introduces the readers to the basic principle of melt-glue cloth. On the basis of melt spinning, the spinneret can be replaced by a spinning device that consists of an outer spinneret (a loop)and an inner spinneret ( a round plate), and between them there is an interval circle on which the centers of the holes are evenly distributed. When the machine is running, the outer spinneret (or the inner one) is fixed, the inner spinneret (or the outer one)is spinning, and a columnar net will be obtained.Then it will be excided with the help of a cutter in transporting it. Finally the once-forming melt-glue cloth will be produced. Compared with the traditional woven fabric, melt-glue cloth has a lot of special features and a bright future of application.展开更多
Highly ordered and porous anodic aluminum oxide templates were prepared. The ordered copper nanowires arrays were assembled in nano-holes of the template by alternating current electrodeposition at lover voltage. The ...Highly ordered and porous anodic aluminum oxide templates were prepared. The ordered copper nanowires arrays were assembled in nano-holes of the template by alternating current electrodeposition at lover voltage. The morphologies of template and copper nano-wires arrays were characterized by means of field emission scanning electron microscope (FESEM) and the crystal structure of copper nano-wires was determined by means of X-ray diffraction. The results indicate that copper nano-wires hold the preferred crystalline orientation along (111), (200), (220) and (331) crystal faces during growth, and the growth of copper nano-wires in the nano-holes of the template is homogenous and continuous.展开更多
Seed‐assisted low alkalinity gel system was developed to explore the organic‐free synthesis of MORzeolite.MOR nanoassemblies with Si/Al ratio(SAR)up to 9.4 and high solid yield(84–94%)weresuccessfully obtained unde...Seed‐assisted low alkalinity gel system was developed to explore the organic‐free synthesis of MORzeolite.MOR nanoassemblies with Si/Al ratio(SAR)up to 9.4 and high solid yield(84–94%)weresuccessfully obtained under controlled low alkalinity conditions.Characterization results demonstratethat the acid strength increases in parallel with the SAR,while the total acid amount and theproton distribution in the main channels and the side pockets are similar for the samples.The protondistribution in the H‐MOR is not straightforwardly related to the Na+distribution in theas‐synthesized MOR,implying the transfer of the protons among the oxygen sites of framework Tatom.Relative to low‐silica samples I‐5.3 and I‐7.4,sample I‐9.4 displays the best mass transferperformance and accessibility of the acid sites by pyridine due to its relatively low Al density andmild dealumination degree.Correspondingly,sample I‐9.4(pyridine‐modified catalyst)shows thebest activity with ca.100%selectivity of methyl acetate(MAc)in the DME carbonylation reaction.The high steady MAc yield(6.8 mmol/g/h)over sample I‐9.4 suggests the promising application ofMOR nanoassemblies synthesized by this economical organic‐free strategy.展开更多
Mycelial growth and yield of two strains ofA. aegerita on different substrates was investigated. Mycelial growth on agar media （PDA, standard, wheat, MEA, CYM, PCA, sawdust extracts： alder and mixture of beech and b...Mycelial growth and yield of two strains ofA. aegerita on different substrates was investigated. Mycelial growth on agar media （PDA, standard, wheat, MEA, CYM, PCA, sawdust extracts： alder and mixture of beech and birch （1：1）） and sawdust substrates （birch, beech, oak, maple, alder） was investigated. Petri dishes （Ф 9 cm） for agar media and biological tubes （18 cm long and Ф 2.5 cm） for sawdust substrates were used. Yield and morphological features were studied on birch, beech and mixture of beech and alder （1：1） sawdust substrates. The temperature of inoculation for agar media and sawdust substrates was 25 ℃. For yielding, when mycelium has completely overgrown the substrate the temperature was decreased to 15-17 ℃ to initiate primordia formation. The cultivation was enlightened 10 h/d with daylight lamps （500 Ix）. One crop was harvested after five weeks. The carpophores of black poplar mushrooms were picked up in clusters. There was no statistically important difference between the mycelial growths of the investigated strains. The best growing agar media were PDA （7.3 cm）, MEA （7.2 cm） and wheat （7.1 cm）, both strains showed the slowest mycelium growth on CYM （5.2 cm）. The mycelial growth on sawdust was the best on the beech （7.1 cm） and birch （6.8 cm） sawdust. The best substrate for cultivation ofA. aegerita was mixture of beech and alder sawdust （39.5 g/100 substrate DM）. The dry yield of carpophores were the highest on beech and alder substrate （3.2 g/100 substrate DM）, but dry matter content was the highest on beech sawdust （15.7%）. The heaviest carpophores were harvested from birch sawdust （3.7 g）; the biggest caps from beech and alder sawdust （3.3 cm）. There were no statistically important differences between the mycelium growth and yielding between both investigated strains.展开更多
Objective:To explore the influence of EBP50(ezrin-radixin-moesin-binding phospho-protein-50) on microfilament cytoskeleton content and distribution in cultured Hela cells, and to investigate the relationship between t...Objective:To explore the influence of EBP50(ezrin-radixin-moesin-binding phospho-protein-50) on microfilament cytoskeleton content and distribution in cultured Hela cells, and to investigate the relationship between the changes in microfilament cytoskeleton localization and EBP50 after PDGF(platelet-derived growth factor) stimulation, and to further clarify the molecular mechanism by which EBP50 suppresses tumor cell proliferation and migration.Methods:pBK-CMV-HAEBP50 wild type recombinant plasmid and pBK-CMV-HA empty vector were transfected into Hela cells.G418 at 350 mg/L was used to screen for cell clones stably expressing EBP50.Western blot was carried out to detect EBP50 expression.Similarities and differences in microfilament cytoskeleton content and distribution in Hela cells transfected with pBK-CMV-HA-EBP50 wild type recombinant plasmid and pBK-CMV-HA empty vector were also treated with PDGF(10 ng/mL and 20 ng/mL, 37 ℃, 15 min) and stained by rhodamine-labeled phalloidin to observe the distribution of microfilament cytoskeleton in the two groups.EBP50 protein distribution in PDGF-stimulated Hela cells was detected by immunofluorescence.Results:Western blot results confirmed that the EBP50 cDNA fragment could express EBP50 in cultured Hela cell lines and that cell lines stably expressing EBP50 were successfully obtained.Western blot and fluorescence results showed that in the cell line transfected with empty vector, the microfilament cytoskeleton was thick, loose, multidirectional and displayed crossing arrangements.The content of microfilament cytoskeleton in the cell line transfected with pBK-CMV-HA-EBP50 was different from that found in the cell line transfected with empty vector.EBP50 expression enhanced microfilament cytoskeleton polymerization into compact thin filaments.Under the stimulation of PDGF, EBP50 migrated to the cell membrane from the cytosol together with microfilament cytoskeleton and co-localized there.Conclusion:EBP50 can change the distribution of microfilament cytoskeleton in cultured Hela cells and can also bind the microfilament cytoskeleton to the cell membrane under the stimulation of PDGF.EBP50 may play a role in the proliferation and migration of tumor cells by influencing the distribution and localization of microfilament cytoskeleton.展开更多
文摘A simple and green technique has been developed to prepare hierarchical biomorphic ZrO2- CeO2, using silkworm silk as the template. Different from traditional immersion technics, the whole synthesis process depends more on the restriction or direction functions of the silkworm silk template. The analytic results showed that ZrO2-CeO2 exhibited a well-crystallized hierarchically interwoven hollow fiber structure with 16-28 μm in diameter. The grain size of the sample calcined at 800 ℃ was about 14 nm. Consequently, the interwoven meshwork at three dimensions is formed due to the direction of biotemplate. The action mechanism is summarily discussed here. It may bring the biomorphic ZrO2-CeO2 nanomaterials with hierarchical interwoven structures to more applications, such as catalysts.
文摘This paper advances a new concept of textile-meltglue cloth, and introduces the readers to the basic principle of melt-glue cloth. On the basis of melt spinning, the spinneret can be replaced by a spinning device that consists of an outer spinneret (a loop)and an inner spinneret ( a round plate), and between them there is an interval circle on which the centers of the holes are evenly distributed. When the machine is running, the outer spinneret (or the inner one) is fixed, the inner spinneret (or the outer one)is spinning, and a columnar net will be obtained.Then it will be excided with the help of a cutter in transporting it. Finally the once-forming melt-glue cloth will be produced. Compared with the traditional woven fabric, melt-glue cloth has a lot of special features and a bright future of application.
文摘Highly ordered and porous anodic aluminum oxide templates were prepared. The ordered copper nanowires arrays were assembled in nano-holes of the template by alternating current electrodeposition at lover voltage. The morphologies of template and copper nano-wires arrays were characterized by means of field emission scanning electron microscope (FESEM) and the crystal structure of copper nano-wires was determined by means of X-ray diffraction. The results indicate that copper nano-wires hold the preferred crystalline orientation along (111), (200), (220) and (331) crystal faces during growth, and the growth of copper nano-wires in the nano-holes of the template is homogenous and continuous.
文摘Seed‐assisted low alkalinity gel system was developed to explore the organic‐free synthesis of MORzeolite.MOR nanoassemblies with Si/Al ratio(SAR)up to 9.4 and high solid yield(84–94%)weresuccessfully obtained under controlled low alkalinity conditions.Characterization results demonstratethat the acid strength increases in parallel with the SAR,while the total acid amount and theproton distribution in the main channels and the side pockets are similar for the samples.The protondistribution in the H‐MOR is not straightforwardly related to the Na+distribution in theas‐synthesized MOR,implying the transfer of the protons among the oxygen sites of framework Tatom.Relative to low‐silica samples I‐5.3 and I‐7.4,sample I‐9.4 displays the best mass transferperformance and accessibility of the acid sites by pyridine due to its relatively low Al density andmild dealumination degree.Correspondingly,sample I‐9.4(pyridine‐modified catalyst)shows thebest activity with ca.100%selectivity of methyl acetate(MAc)in the DME carbonylation reaction.The high steady MAc yield(6.8 mmol/g/h)over sample I‐9.4 suggests the promising application ofMOR nanoassemblies synthesized by this economical organic‐free strategy.
文摘Mycelial growth and yield of two strains ofA. aegerita on different substrates was investigated. Mycelial growth on agar media （PDA, standard, wheat, MEA, CYM, PCA, sawdust extracts： alder and mixture of beech and birch （1：1）） and sawdust substrates （birch, beech, oak, maple, alder） was investigated. Petri dishes （Ф 9 cm） for agar media and biological tubes （18 cm long and Ф 2.5 cm） for sawdust substrates were used. Yield and morphological features were studied on birch, beech and mixture of beech and alder （1：1） sawdust substrates. The temperature of inoculation for agar media and sawdust substrates was 25 ℃. For yielding, when mycelium has completely overgrown the substrate the temperature was decreased to 15-17 ℃ to initiate primordia formation. The cultivation was enlightened 10 h/d with daylight lamps （500 Ix）. One crop was harvested after five weeks. The carpophores of black poplar mushrooms were picked up in clusters. There was no statistically important difference between the mycelial growths of the investigated strains. The best growing agar media were PDA （7.3 cm）, MEA （7.2 cm） and wheat （7.1 cm）, both strains showed the slowest mycelium growth on CYM （5.2 cm）. The mycelial growth on sawdust was the best on the beech （7.1 cm） and birch （6.8 cm） sawdust. The best substrate for cultivation ofA. aegerita was mixture of beech and alder sawdust （39.5 g/100 substrate DM）. The dry yield of carpophores were the highest on beech and alder substrate （3.2 g/100 substrate DM）, but dry matter content was the highest on beech sawdust （15.7%）. The heaviest carpophores were harvested from birch sawdust （3.7 g）; the biggest caps from beech and alder sawdust （3.3 cm）. There were no statistically important differences between the mycelium growth and yielding between both investigated strains.
基金Supported by grants from the National Natural Science Foundation ofChina (No. 30572183 and 30772573)Beijing Educational Committee Foundation (No. KZ200610025013)+2 种基金the New Century Excellent Talentsin University of China (No. NCEF-06-0184)Excellent Talents Foundation in Beijing (No. 20071D0501800253)Beijing Novagramme Foundation (No. 2008B58 [MS1]).
文摘Objective:To explore the influence of EBP50(ezrin-radixin-moesin-binding phospho-protein-50) on microfilament cytoskeleton content and distribution in cultured Hela cells, and to investigate the relationship between the changes in microfilament cytoskeleton localization and EBP50 after PDGF(platelet-derived growth factor) stimulation, and to further clarify the molecular mechanism by which EBP50 suppresses tumor cell proliferation and migration.Methods:pBK-CMV-HAEBP50 wild type recombinant plasmid and pBK-CMV-HA empty vector were transfected into Hela cells.G418 at 350 mg/L was used to screen for cell clones stably expressing EBP50.Western blot was carried out to detect EBP50 expression.Similarities and differences in microfilament cytoskeleton content and distribution in Hela cells transfected with pBK-CMV-HA-EBP50 wild type recombinant plasmid and pBK-CMV-HA empty vector were also treated with PDGF(10 ng/mL and 20 ng/mL, 37 ℃, 15 min) and stained by rhodamine-labeled phalloidin to observe the distribution of microfilament cytoskeleton in the two groups.EBP50 protein distribution in PDGF-stimulated Hela cells was detected by immunofluorescence.Results:Western blot results confirmed that the EBP50 cDNA fragment could express EBP50 in cultured Hela cell lines and that cell lines stably expressing EBP50 were successfully obtained.Western blot and fluorescence results showed that in the cell line transfected with empty vector, the microfilament cytoskeleton was thick, loose, multidirectional and displayed crossing arrangements.The content of microfilament cytoskeleton in the cell line transfected with pBK-CMV-HA-EBP50 was different from that found in the cell line transfected with empty vector.EBP50 expression enhanced microfilament cytoskeleton polymerization into compact thin filaments.Under the stimulation of PDGF, EBP50 migrated to the cell membrane from the cytosol together with microfilament cytoskeleton and co-localized there.Conclusion:EBP50 can change the distribution of microfilament cytoskeleton in cultured Hela cells and can also bind the microfilament cytoskeleton to the cell membrane under the stimulation of PDGF.EBP50 may play a role in the proliferation and migration of tumor cells by influencing the distribution and localization of microfilament cytoskeleton.
