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猪第二极体核物质支持胚胎早期发育的研究 被引量:2
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作者 于建宁 王公金 +2 位作者 周晓龙 徐小波 范必勤 《江苏农业学报》 CSCD 北大核心 2012年第5期1079-1082,共4页
为了探讨猪第二极体是否具有支持胚胎发育的全能性,采用显微操作的方法将猪第二极体移入去除雌原核的原核胚中,电融合完成重组,体外观察重组胚胎的发育情况。结果显示:猪卵母细胞体外受精4 h后即有第二极体排出;受精20 h后,50.6%的受精... 为了探讨猪第二极体是否具有支持胚胎发育的全能性,采用显微操作的方法将猪第二极体移入去除雌原核的原核胚中,电融合完成重组,体外观察重组胚胎的发育情况。结果显示:猪卵母细胞体外受精4 h后即有第二极体排出;受精20 h后,50.6%的受精卵排出第二极体,且82.2%的第二极体具有活性。将有活性的第二极体与去除雌原核的原核胚重组后,重组胚胎能够发育到8-细胞期,并且重组胚胎发育的8-细胞与体处受精胚胎发育的8-细胞在形态上没有明显差别。表明猪第二极体核物质能够支持胚胎发育。 展开更多
关键词 第二 极体活性 重组胚胎
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Comparative investigation on spindle behavior and MPF activity changes during oocyte maturation between gynogenetic and amphimictic crucian carp 被引量:4
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作者 YANG ZHONG AN QUN HUA LI +1 位作者 YU FENG WANG JIAN FANG GUI (State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China) 《Cell Research》 SCIE CAS CSCD 1999年第2期145-154,共10页
The spindle behavior and MPF activity changes in the progression of oocyte maturation were investigated and compared with cytological observation and kinase assay between gynogenetic silver crucian carp and amphimicti... The spindle behavior and MPF activity changes in the progression of oocyte maturation were investigated and compared with cytological observation and kinase assay between gynogenetic silver crucian carp and amphimictic colored crucian carp. MPF activity was measured by using histone H1 as phosphorylation substrate. There were two similar oscillatory MPF kinase activity changes during oocyte maturation in two kinds of fishes with different reproductive modes, but there existed some subtle difference between them. The subtle difference was that the first peak of MPF kinase activity was kept to a longerlasting time in the gynogenetic silver crucian carp than in the amphimictic colored crucian carp. It was suggested that the difference may be related to the spindle behavior changes, such as tripolar spindle formation and spindle rearrangement in the gynogenetic crucian carp. 展开更多
关键词 GYNOGENESIS silver crucian carp oocyte maturation MPF kinase tripolar spindle MEIOSIS
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WB crystals with oxidized surface as counter electrode in dye-sensitized solar cells
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作者 Jian Pan Chao Zhen +2 位作者 Lianzhou Wang Gang Liu Hui-Ming Cheng 《Science Bulletin》 SCIE EI CAS CSCD 2017年第2期114-118,共5页
Tungsten boride(WB) crystals, whose surface tends to be oxidized when exposed to air, were demonstrated to have a comparable activity to platinum as counter electrode material in dye-sensitized solar cells. The synerg... Tungsten boride(WB) crystals, whose surface tends to be oxidized when exposed to air, were demonstrated to have a comparable activity to platinum as counter electrode material in dye-sensitized solar cells. The synergistic effect of both catalytically active surface layer WOxand electronically conductive internal WB is considered to be responsible for the high activity of the WB crystals. 展开更多
关键词 Counter electrode Tungsten boride Dye-sensitized solar cell Tungsten oxide
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